Killing of trypanosomes by the human haptoglobin-related protein.

African trypanosomes cause disease in humans and animals. Trypanosoma brucei brucei affects cattle but not humans because of its sensitivity to a subclass of human high density lipoproteins (HDLs) called trypanosome lytic factor (TLF). TLF contains two apolipoproteins that are sufficient to cause lysis of T. b. brucei in vitro. These proteins were identified as the human haptoglobin-related protein and paraoxonase-arylesterase. An antibody to haptoglobin inhibited TLF activity. TLF was shown to exhibit peroxidase activity and to be inhibited by catalase. These results suggest that TLF kills trypanosomes by oxidative damage initiated by its peroxidase activity.

[1]  S. Hajduk,et al.  Reconstitution of the trypanolytic factor from components of a subspecies of human high-density lipoproteins. , 1995, Molecular and biochemical parasitology.

[2]  S. Hajduk,et al.  Endocytosis of a cytotoxic human high density lipoprotein results in disruption of acidic intracellular vesicles and subsequent killing of African trypanosomes , 1994, The Journal of cell biology.

[3]  V. Armstrong,et al.  Lipoproteins and hydrolysis of organophosphorus compounds. , 1993, Chemico-biological interactions.

[4]  H. Brzeski,et al.  Identification of the products of the haptoglobin-related gene. , 1990, Biochimica et biophysica acta.

[5]  S. Hajduk,et al.  Lysis of Trypanosoma brucei by a toxic subspecies of human high density lipoprotein. , 1989, The Journal of biological chemistry.

[6]  F. Kierszenbaum,et al.  Interaction of human eosinophils or neutrophils with Trypanosoma cruzi in vitro causes bystander cardiac cell damage. , 1989, Immunology.

[7]  H. Nakajima,et al.  Intrahepatocellular site of the catabolism of heme and globin moiety of hemoglobin-haptoglobin after intravenous administration to rats. , 1988, The Journal of biological chemistry.

[8]  N. Maeda,et al.  Complex events in the evolution of the haptoglobin gene cluster in primates. , 1988, The Journal of biological chemistry.

[9]  N. Maeda,et al.  Nucleotide sequence of the haptoglobin and haptoglobin-related gene pair. The haptoglobin-related gene contains a retrovirus-like element. , 1985, The Journal of biological chemistry.

[10]  F. Yang,et al.  Identification and characterization of human haptoglobin cDNA. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[11]  H. Nakajima,et al.  Hemoglobin-haptoglobin receptor in rat liver plasma membrane. , 1980, The Journal of biological chemistry.

[12]  M. Rifkin.  Trypanosoma brucei: some properties of the cytotoxic reaction induced by normal human serum. , 1978, Experimental parasitology.

[13]  S. Meshnick,et al.  An approach to the development of new drugs for African trypanosomiasis , 1978, The Journal of experimental medicine.

[14]  S. Meshnick,et al.  Heme lysis of the bloodstream forms of Trypanosoma brucei. , 1977, Biochemical pharmacology.

[15]  M. Kirschner,et al.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. , 1977, The Journal of biological chemistry.

[16]  H. Baernstein.  A review of electron transport mechanisms in parasitic protozoa. , 1963, The Journal of parasitology.

[17]  O. Smithies,et al.  Human haptoglobins: estimation and purification. , 1959, The Biochemical journal.