Surface Termination Effect of Boron‐Doped Diamond on the Electrochemical Oxidation of Adenosine Phosphate

Electrochemical oxidation of adenosine phosphates, including adenosine 5′-monophosphate (AMP), adenosine 5′-diphosphate (ADP), and adenosine 5′-triphosphate (ATP), have been studied using boron-doped diamond (BDD) electrodes. Cyclic voltammograms in phosphate buffer solution pH 7.4 showed a typical oxidation peak at the potential of around +1.3 V (vs. Ag/AgCl) attributable to the oxidation of adenine base moiety. This peak appears at both cathodically and anodically treated BDD. However, investigation at low pH showed, whereas the oxidation peak could be well observed for the oxidation of each AMP, ADP, and ATP at cathodically treated BDD, it was only found for the oxidation of AMP at anodically-treted BDD electrodes. Linear calibration curve can be achieved in the concentration range of 20 to 200 μM with an average detection limit of 2 μM (S/N=3). Furthermore, cathodic treatment has demonstrated its ability to maintain the electrode surface from biofouling, resulting in the excellent stability of current responses.

[1]  R. Trouillon,et al.  Comparison of glassy carbon and boron doped diamond electrodes: Resistance to biofouling , 2010 .

[2]  G. Pacey,et al.  Determination of ATP using chelation-enhanced fluorescence. , 1996, Talanta.

[3]  G. Dryhurst.  Dicarbonium Ions as Products of Electrochemical Oxidation of Biologically Important Purines at the Pyrolytic Graphite Electrode , 1969 .

[4]  T. Yao,et al.  The Electrochemical Oxidation of Aminopurines and Their Hydroxy Derivatives at the Glassy Carbon Electrode , 1979 .

[5]  K. Yamanaka,et al.  Potentiometric method for the determination of adenosine-5′-triphosphate , 1993 .

[6]  G. Guilbault,et al.  Glucose oxidase/hexokinase electrode for the determination of ATP , 1997 .

[7]  Atsushi Hashimoto,et al.  Mid-Infrared Spectroscopic Measurement of Ionic Dissociative Materials in the Metabolic Pathway , 2003, Applied spectroscopy.

[8]  F. Scheller,et al.  Enzyme electrodes for ADP/ATP with enhanced sensitivity due to chemical amplification and intermediate accumulation , 1991 .

[9]  Rajendra N. Goyal,et al.  Electrochemical investigations of adenosine at solid electrodes , 2002 .

[10]  Kazuhito Hashimoto,et al.  Electrochemical Behavior of Highly Conductive Boron‐Doped Diamond Electrodes for Oxygen Reduction in Alkaline Solution , 1998 .

[11]  P. Elving,et al.  Electrochemical oxidation-reduction paths for pyrimidine, cytosine, purine and adenine Correlation and application. , 1969, Talanta.

[12]  S. Kitazawa,et al.  Fabrication, characterization, and application of boron-doped diamond microelectrodes for in vivo dopamine detection. , 2007, Analytical chemistry.

[13]  P. Elving,et al.  Electrochemical Oxidation of Adenine: Reaction Products and Mechanisms , 1968 .

[14]  T. Ivandini,et al.  Yeast-based Biochemical Oxygen Demand Sensors Using Gold-modified Boron-doped Diamond Electrodes , 2015, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry.

[15]  Yasuaki Einaga,et al.  Development of a biochemical oxygen demand sensor using gold-modified boron doped diamond electrodes. , 2012, Analytical chemistry.

[16]  Chunhai Fan,et al.  A target-responsive electrochemical aptamer switch (TREAS) for reagentless detection of nanomolar ATP. , 2007, Journal of the American Chemical Society.

[17]  A. Fujishima,et al.  Electrochemical selectivity for redox systems at oxygen-terminated diamond electrodes ☆ , 1999 .

[18]  S. C. B. Oliveira,et al.  Boron doped diamond electrode pre-treatments effect on the electrochemical oxidation of dsDNA, DNA bases, nucleotides, homopolynucleotides and biomarker 8-oxoguanine , 2010 .

[19]  T. Ivandini,et al.  Electrochemical oxidation of underivatized-nucleic acids at highly boron-doped diamond electrodes. , 2003, The Analyst.

[20]  C. Brett,et al.  Adsorption of Guanine, Guanosine, and Adenine at Electrodes Studied by Differential Pulse Voltammetry and Electrochemical Impedance , 2002 .

[21]  T. Ivandini,et al.  Electrochemical oxidation of oxalic acid at highly boron-doped diamond electrodes. , 2006, Analytical chemistry.

[22]  S. Szunerits,et al.  Nucleosides and ODN electrochemical detection onto boron doped diamond electrodes. , 2004, Bioelectrochemistry.

[23]  T. Ivandini,et al.  Sensitive Electrochemical Detection of Oxalate at a Positively Charged Boron‐Doped Diamond Surface , 2008 .

[24]  A. Schoffstall,et al.  Reversed-phase high-performance liquid chromatographic separation of nucleosides and nucleotides , 1983 .

[25]  S. R. Biaggio,et al.  Electrochemical determination of bisphenol A using a boron-doped diamond electrode , 2012 .

[26]  Y. Umezawa,et al.  Na+,K+-ATPase-based bilayer lipid membrane sensor for adenosine 5′-triphosphate , 1993 .

[27]  A. Soldatkin,et al.  Amperometric biosensor system for simultaneous determination of adenosine-5'-triphosphate and glucose. , 2014, Analytical chemistry.

[28]  Rajendra N. Goyal,et al.  Oxidation chemistry of adenine and hydroxyadenines at pyrolytic graphite electrodes , 1991 .

[29]  H. Girard,et al.  Effect of anodic and cathodic treatments on the charge transfer of boron doped diamond electrodes , 2007 .

[30]  R. Rocha‐Filho,et al.  Square-wave voltammetric determination of hydroxychloroquine in pharmaceutical and synthetic urine samples using a cathodically pretreated boron-doped diamond electrode , 2014 .

[31]  Mun'delanji C. Vestergaard,et al.  Electroanalytical Characterization of Adenosine Mono-, Di- and Triphosphate Oxidation on Carbon Electrode , 2008 .

[32]  R. Rocha‐Filho,et al.  Square-wave voltammetric determination of bezafibrate in pharmaceutical formulations using a cathodically pretreated boron-doped diamond electrode. , 2013, Talanta.

[33]  Yujing Guo,et al.  Porphyrin functionalized graphene nanosheets-based electrochemical aptasensor for label-free ATP detection , 2012 .

[34]  Akira Fujishima,et al.  Simultaneous detection of purine and pyrimidine at highly boron-doped diamond electrodes by using liquid chromatography. , 2007, Talanta.

[35]  J. Galligan,et al.  Electrochemical activation of diamond microelectrodes: implications for the in vitro measurement of serotonin in the bowel. , 2014, The Analyst.

[36]  Michelle L Rogers,et al.  ATP microelectrode biosensor for stable long-term in vitro monitoring from gastrointestinal tissue. , 2011, Biosensors & bioelectronics.

[37]  Martin A. M. Gijs,et al.  Cathodic pretreatment improves the resistance of boron-doped diamond electrodes to dopamine fouling , 2014 .

[38]  K. Yoshimi,et al.  In vivo assessment of cancerous tumors using boron doped diamond microelectrode , 2012, Scientific Reports.

[39]  P E Stanley,et al.  A review of bioluminescent ATP techniques in rapid microbiology. , 1989, Journal of bioluminescence and chemiluminescence.