Correlation of plasma progesterone concentrations to ovarian H-type lipase activity during pseudopregnancy in the rat.

Conditions for extraction and assay of hepatic type (H-type) lipase from rat ovaries were studied. An alkaline buffer with protease inhibitors and detergents gave the most efficient extraction. The specificity of the assays was ascertained using antiserum to H-type lipase from heparin perfusates of rat livers. H-type lipase activity was determined in ovarian compartments during pseudopregnancy (1-13 days) as well as during the ensuing period of luteal regression (Day 17). The activity was low in the luteal compartment immediately after ovulation, increased 6-fold to a maximum between Day 5 and Day 8 and then decreased again. This is similar to previously known changes in blood flow. There was a significant correlation between luteal H-type lipase activity and plasma progesterone regardless of luteal age. In contrast, neither the activity in the remainder of the ovary nor the activity in plasma changed during the luteal phase or correlated to plasma progesterone. Injection of heparin at the height of the luteal cycle (Day 8) caused a pronounced decrease in luteal lipase and in plasma progesterone. These studies suggest that the H-type lipase activity has an important role in luteal steroidogenesis, probably to facilitate uptake of cholesterol from lipoproteins.

[1]  T. Waldron.  "Further studies ...' , 1991, The Lancet.

[2]  H. Jansen,et al.  L-type lipase activity in ovaries of superovulated rats Relation to cholesterol homeostasis , 1988, Molecular and Cellular Endocrinology.

[3]  D. Ledbetter,et al.  Human hepatic lipase. Cloned cDNA sequence, restriction fragment length polymorphisms, chromosomal localization, and evolutionary relationships with lipoprotein lipase and pancreatic lipase. , 1988, The Journal of biological chemistry.

[4]  R. Davis,et al.  Synthesis of hepatic lipase in liver and extrahepatic tissues. , 1987, Journal of lipid research.

[5]  P. Belfrage,et al.  Immunological evidence for the presence of hormone-sensitive lipase in rat tissues other than adipose tissue. , 1987, Biochemical and biophysical research communications.

[6]  G. Selstam,et al.  Blood flow changes and vascular appearance in preovulatory follicles and corpora lutea in immature, pregnant mare's serum gonadotropin-treated rats. , 1987, Biology of reproduction.

[7]  R. Sprengel,et al.  Human hepatic triglyceride lipase: cDNA cloning, amino acid sequence and expression in a cultured cell line. , 1987, Differentiation; research in biological diversity.

[8]  G. Selstam,et al.  Influence of oxygen tension and substrates on basal and luteinizing hormone stimulated progesterone production and energy metabolism by isolated corpora lutea of adult pseudopregnant rats. , 1987, Acta physiologica Scandinavica.

[9]  M. Komaromy,et al.  Cloning of rat hepatic lipase cDNA: evidence for a lipase gene family. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[10]  G. Selstam,et al.  Redistribution of ovarian blood flow after injection of human chorionic gonadotropin and luteinizing hormone in the adult pseudopregnant rat. , 1987, Endocrinology.

[11]  T. Olivecrona,et al.  Mouse preheparin plasma contains high levels of hepatic lipase with low affinity for heparin. , 1986, Biochimica et biophysica acta.

[12]  H. Jansen,et al.  Localization of the salt-resistant heparin-releasable lipase in the rat liver, adrenal and ovary. , 1986, European journal of cell biology.

[13]  T. Olivecrona,et al.  Effect of combined lipase deficiency (cld/cld) on hepatic and lipoprotein lipase activities in liver and plasma of newborn mice. , 1986, Biochimica et biophysica acta.

[14]  T. Olivecrona,et al.  Distribution of lipoprotein lipase and hepatic lipase between plasma and tissues: effect of hypertriglyceridemia. , 1985, Biochimica et biophysica acta.

[15]  H. Jansen,et al.  Localization of liver-type lipase in rat ovaries and its activity during the estrous cycle and lactation , 1985, Molecular and Cellular Endocrinology.

[16]  M. Phillips,et al.  Mechanism of the hepatic lipase induced accumulation of high-density lipoprotein cholesterol by cells in culture. , 1985, Biochemistry.

[17]  B. Murphy,et al.  Binding of high-density lipoproteins to luteal membranes: the role of prolactin, luteinizing hormone, and circulating lipoproteins. , 1985, Biology of reproduction.

[18]  A. Dharmarajan,et al.  Quantitative ultrastructural characteristics relating to transport between luteal cell cytoplasm and blood in the corpus luteum of the pregnant rat. , 1985, The American journal of anatomy.

[19]  T. Olivecrona,et al.  Hepatic and extrahepatic uptake of intravenously injected lipoprotein lipase. , 1984, Biochimica et biophysica acta.

[20]  T. Olivecrona,et al.  Lipoprotein lipase mediated uptake of non-degradable ether analogues of phosphatidylcholine and cholesteryl ester by cultured cells. , 1984, Biochimica et biophysica acta.

[21]  G. S. Greenwald,et al.  Further studies on in vitro steroidogenesis by luteal cells from long-term hypophysectomized rats. , 1984, Biology of reproduction.

[22]  T. Olivecrona,et al.  The effects of pH and salt on the lipid binding and enzyme activity of lipoprotein lipase. , 1983, Biochimica et biophysica acta.

[23]  J. Gunnet,et al.  The mating-induced release of prolactin: a unique neuroendocrine response. , 1983, Endocrine reviews.

[24]  J. Strauss,et al.  The role of lipoproteins in steroidogenesis and cholesterol metabolism in steroidogenic glands. , 1982, Endocrine reviews.

[25]  J. Strauss,et al.  Binding of human high density lipoproteins to membranes of luteinized rat ovaries. , 1981, Journal of steroid biochemistry.

[26]  H. Jansen,et al.  Heparin-releasable lipase activity of rat adrenals, ovaries and testes. , 1981, The Biochemical journal.

[27]  I. Rothchild.  The regulation of the mammalian corpus luteum. , 1981, Recent progress in hormone research.

[28]  H. Jansen,et al.  Heparin-releasable (liver) lipase(s) may play a role in the uptake of cholesterol by steroid-secreting tissues , 1980 .

[29]  J. Strauss,et al.  Effect of reduced blood cholesterol on sterol and steroid metabolism by rat luteal tissue. , 1979, Endocrinology.

[30]  N. Schwartz,et al.  Measurement of serum steroid and gonadotropin levels and uterine and ovarian variables throughout 4 day and 5 day estrous cycles in the rat. , 1979, Biology of reproduction.

[31]  J. Dietschy,et al.  Relative importance of high and low density lipoproteins in the regulation of cholesterol synthesis in the adrenal gland, ovary, and testis of the rat. , 1978, The Journal of biological chemistry.

[32]  Y. Salomon,et al.  Heparin: A potent inhibitor of ovarian luteinizing hormone‐sensitive adenylate cyclase , 1977, FEBS letters.

[33]  P. Strålfors,et al.  Hormone‐sensitive lipase of rat adipose tissue: Identification and some properties of the enzyme protein , 1977, FEBS letters.

[34]  H. Herlitz,et al.  Induction by PGF2α of 20α-hydroxysteroid dehydrogenase in first generation corpora lutea of the rat , 1975, Molecular and Cellular Endocrinology.

[35]  T. Olivecrona,et al.  Serum-stimulated lipases (lipoprotein lipases). Immunological crossreaction between the bovine and the human enzymes. , 1975, Biochimica et biophysica acta.

[36]  G. Pepe,et al.  A comparative study of serum progesterone levels in pregnancy and in various types of pseudopregnancy in the rat. , 1974, Endocrinology.

[37]  D. Szarowski,et al.  Progravid phase of the rat reproductive cycle: day to day changes in peripheral plasma progestin concentrations. , 1973, Endocrinology.

[38]  P. Belfrage,et al.  Simple liquid-liquid partition system for isolation of labeled oleic acid from mixtures with glycerides. , 1969, Journal of lipid research.

[39]  Herbert M. Evans,et al.  The oestrous cycle in the rat and its associated phenomena , 1922 .