Morphological and molecular investigations of Paramecium schewiakoffi sp. nov. (Ciliophora, Oligohymenophorea) and current status of distribution and taxonomy of Paramecium spp.

Paramecium schewiakoffi sp. nov. is described from a pond in Shanghai, China. It is a freshwater species belonging to the “aurelia” subgroup of the genus. It is of similar size and shape to P. jenningsi, but has a single large micronucleus of the “chromosomal” morphological type, while P. jenningsi has two smaller micronuclei. The general morphology, morphometric characteristics and nuclear reorganization pattern, a random amplified polymorphic DNA (RAPD) fingerprint pattern, and the small subunit rRNA gene sequence are presented for the species. Comparison of P. schewiakoffi with the other species of Paramecium indicates that it is a valid new species of the genus. Geographical locations reported for many Paramecium species do not support the theory that all ciliates have a cosmopolitan distribution. It is proposed that, in an extension of Jankowski's earlier suggestion, the genus Paramecium should be subdivided into four subgenera: Chloroparamecium, Helianter, Cypriostomum and Paramecium, on the basis of morphometric, biological and molecular differences.

[1]  F. Sanger,et al.  DNA sequencing with chain-terminating inhibitors. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[2]  L. L. Woodruff.  THE STRUCTURE, LIFE HISTORY, AND INTRAGENERIC RELATIONSHIPS OF PARAMECIUM CALKINSI, SP. NOV , 1921 .

[3]  S. Fokin,et al.  BRACKISH WATER PARAMECIUM SPECIES AND PARAMECIUM POLYCARYUM. MORPHOMETRICAL ANALYSIS AND SOME BIOLOGICAL PECULIARITIES , 1999 .

[4]  S. Fokin,et al.  Data on the occurrence of species of the Paramecium aurelia complex world-wide , 2000 .

[5]  T. Nerad,et al.  Paramecium sonneborni n. sp., a New Member of the Paramecium aurelia Species-Complex† , 1983 .

[6]  M. Strüder-Kypke,et al.  Phylogenetic relationships of the genus Paramecium inferred from small subunit rRNA gene sequences. , 2000, Molecular phylogenetics and evolution.

[7]  J. Mignot,et al.  Précis de protistologie , 1987 .

[8]  F. Dahl,et al.  Wimpertiere oder Ciliata (Infusoria) , 1930 .

[9]  J. Felsenstein.  Phylogenies from molecular sequences: inference and reliability. , 1988, Annual review of genetics.

[10]  W. Foissner.  Protist diversity: estimates of the near-imponderable. , 1999, Protist.

[11]  T. M. Sonneborn.  The Paramecium aurelia Complex of Fourteen Sibling Species , 1975 .

[12]  T. Fenchel,et al.  Protozoan diversity: converging estimates of the global number of free-living ciliate species. , 1998, Protist.

[13]  T. Stoeck,et al.  Paramecium duboscqui Chatton, Brachon, 1933. distribution, ecology and taxonomy , 1999 .

[14]  T. Stoeck,et al.  Fast and Accurate Identification of European Species of the Paramecium aurelia Complex by RAPD-Fingerprints , 1998, Microbial Ecology.

[15]  D. Wenrich.  Eight well-defined species of Paramecium (Pro-tozoa, Ciliata) , 1928 .

[16]  R. Wichterman.  The Biology of Paramecium , 1986, Springer US.

[17]  F. Lapointe,et al.  RAPD problems in phylogenetics , 1996 .

[18]  P. Fuerst,et al.  Discovery of group I introns in the nuclear small subunit ribosomal RNA genes of Acanthamoeba. , 1994, Nucleic acids research.

[19]  C. G. Ehrenberg.  Die Infusionsthierchen als Vollkommene Organismen. , 1838 .

[20]  S. Fokin,et al.  Paramecium morphometric analysis and taxonomy , 2000 .

[21]  Jochen Schaumburg,et al.  Identification and ecology of limnetic plankton ciliates , 1999 .

[22]  T. Fenchel,et al.  Biodiversity at the Microbial Level: The Number of Free-Living Ciliates in the Biosphere , 1996, The Quarterly Review of Biology.

[23]  J. Powers,et al.  A NEW SPECIES OF PARAMECIUM (P. MULTIMICRONUCLEATA) EXPERIMENTALLY DETERMINED , 1910 .

[24]  T. Stoeck,et al.  Rediscovery of Paramecium nephridiatum Gelei, 1925 and its Characteristics , 1999 .

[25]  S. Fokin,et al.  Nuclear Reorganization Variety in Paramecium (Ciliophora: Peniculida) and its Possible Evolution , 2001 .

[26]  P. R. Earl,et al.  Paramecium jenningsi, n. sp. , 1958 .

[27]  L. L. Woodruff,et al.  Paramecium polycaryum, sp. nov , 1923 .

[28]  W. V. Wagtendonk.  Paramecium: A current survey , 1974 .

[29]  T. Fenchel,et al.  Divergent perspectives on protist species richness. , 1999, Protist.

[30]  S. Fokin.  Morphological diversity of the micronuclei in Paramecium , 1997 .

[31]  M. Strüder-Kypke,et al.  Phylogenetic Relationships of the Subclass Peniculia (Oligohymenophorea, Ciliophora) Inferred from Small Subunit rRNA Gene Sequences , 2000, The Journal of eukaryotic microbiology.

[32]  Sabine Agatha,et al.  Soil ciliates (protozoa, ciliophora) from Namibia (Southwest Africa), with emphasis on two contrasting environments, the Etosha Region and the Namib Desert , 2002 .

[33]  S. Fokin.  Morphology of the contractile vacuoles in ciliated protozoa of the genus paramecium hymenostomatida peniculina as a species specific character , 1986 .

[34]  K. Hiwatashi.  Determination and inheritance of mating type in Paramecium caudatum. , 1968, Genetics.

[35]  S. Fokin,et al.  Resistance of Paramecium Species (Ciliophora, Peniculia) to Salinity of Environment , 1999 .

[36]  René-Édouard Claparède,et al.  Etudes sur les infusoires et les rhizopodes , 1860 .

[37]  J. O. Corliss,et al.  Silver impregnation of ciliated protozoa by the Chatton-Lwoff technic. , 1953, Stain technology.

[38]  W. Schewiakoff.  Über die geographische Verbreitung der Süsswasser-Protozoën , 2022 .