Histomorphometric evaluation of osteons inside the compacta of long bones diaphysis. Implication in pathology

The quantitative, micro-anatomic assessment of Havers systems within the structure of diaphysis compacta of long bones imposes the acknowledgement and visualization of reference landmarks for morphometric. Authors consider that the utilization of histolo-physics methods (examination in polarized light and/or interferential contrast) assures the visualization of histotopographic landmarks necessary for performing these measurements. Histo-physics analysis of longitudinal sections through tibia diaphysis compacta allowed authors to emphasize the spatial alternation of isotropic columns of osteons lamellas with Havers central canals, with anisotropic columns and inter-osteon spaces. Authors consider that the measurement of osteon diameters in the interval between two inter-osteon isotropic columns ensures the constancy of osteons' geometry rigorous evaluation.