Regeneración de plantas de cinco variedades de Phaseolus vulgaris L. vía organogénesis directa

The beans are an important source for human consumption in Cuba due their protein content. For this reason; the search for cultivars with greater potential is a prominent challenge in this specie. Tissue culture in Phaseolus vulgaris L. is very difficult, among other things, due to its genotype-dependent condition. The aim of this study was to evaluate the regeneration of plants via direct organogenesis in five genotypes of P. vulgaris . The genotypes studied were `ICA Pijao', `BAT 93', `BAT 482', `BAT 304' and `CIAP7247F'. The investigation showed that genotypes `BAT 304' and `Pijao ICA' had the highest values of explants with multiple buds induction. In the variable number of multiple buds per explant, the highest values were obtained in `CIAP7247F' and `BAT 482' genotypes. In `BAT 93' callus formation was so profuse that reduced the number of explants with buds induced. Plant regeneration was another variable that differed in the genotypes studied. `CIAP7247F' showed the greater number of shoots per explant while genotypes that showed the lowest values were `BAT 304' and `BAT 93'. The effect of the variety in this species was shown during the tissues culture process. Keywords: beans, in vitro culture, multiple buds.

[1]  H. Guzmán-Maldonado,et al.  AN IMPROVED METHOD FOR in vitro REGENERATION OF COMMON BEAN (Phaseolus vulgaris L.) , 2010 .

[2]  J. M. Valverde,et al.  In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate , 2010 .

[3]  Villordo-Pineda,et al.  ORGANOGENIC PLANT REGENERATION SYSTEM FOR THE COMMON BEAN , 2010 .

[4]  M. Sticklen,et al.  In vitro regeneration and morphogenesis studies in common bean , 2009, Plant Cell, Tissue and Organ Culture (PCTOC).

[5]  I. I. Ozyigit,et al.  Phenolic changes during in vitro organogenesis of cotton ( Gossypium hirsutum L.) shoot tips , 2008 .

[6]  N. Veitía,et al.  Regeneración de plantas vía organogénesis directa en Phaseolus vulgaris L. Plants regeneration via direct organogenesis in Phaseolus vulgaris L , 2008 .

[7]  G. Hernández,et al.  Regeneration of different cultivars of common bean (Phaseolus vulgaris L.) via indirect organogenesis , 2008, Plant Cell, Tissue and Organ Culture.

[8]  Chang-Sheng Wang,et al.  Organogenesis of Phaseolus angularis L.: high efficiency of adventitious shoot regeneration from etiolated seedlings in the presence of N6-benzylaminopurine and thidiazuron , 2006, Plant Cell, Tissue and Organ Culture.

[9]  P. Delgado-Sánchez,et al.  An organogenic plant regeneration system for common bean (Phaseolus vulgaris L.) , 2006 .

[10]  M. Veltcheva,et al.  In vitro regeneration and genetic transformation of common bean (Phaseolus vulgaris L.)—Problems and progress , 2005 .

[11]  M. Davey,et al.  Efficient in vitro shoot regeneration responses of Phaseolus vulgaris and P. coccineus , 1998, Euphytica.

[12]  Cruz de Carvalho MH,et al.  Efficient whole plant regeneration of common bean (Phaseolus vulgaris L.) using thin-cell-layer culture and silver nitrate. , 2000, Plant science : an international journal of experimental plant biology.

[13]  M. Montagu,et al.  Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray , 1997, Theoretical and Applied Genetics.

[14]  A. Rubluo,et al.  In vitro Culture of Shoot Apical Meristems of Various Phaseolus Species and Cultivars , 1985 .