The effect of aspartame and pH changes on the erosive potential of cola drinks in bovine enamel: An in vitro study

Background The aim of this study was to clarify the mechanism behind the different erosive potential of regular and light cola drinks: pH difference and/or aspartame presence. Material and Methods Sixty bovine enamel blocks were randomly divided into 5 groups: RC - regular cola, RCpH - addition of base to increase regular cola pH, RCAS - addition of aspartame to regular cola, LC - light cola, and LCpH - addition of acid to decrease light cola pH. Two-thirds of the blocks surface was coated with nail varnish for reference. The samples were daily subjected to four erosive challenges for 2 minutes. Between the erosive challenges (2h) and overnight the samples were maintained in artificial saliva. The response variable was the percentage surface hardness change (%SHC) after 1st experimental day and enamel surface loss (µm) measured at the 5th day by profilometry. Data were analyzed by ANOVA and Tukey’s test (p<0.05). Results Independently of the cola modifications, all groups promoted similar hardness change of enamel surface. RC promoted higher enamel loss (6.69±0.71µm) than LC (4.80±0.77µm). The acid addition to light cola (LCpH: 6.60±1.78µm) significantly enhanced its erosive potential, which did not differ from RC. The base addition to regular cola (RCpH-4.00±0.64µm) resulted in similar wear to LC. The addition of aspartame to the regular cola (RCAS 5.44±0.65µm) resulted in similar wear to LC and RC. Conclusions The data suggest that the pH alteration has a major impact on the erosive potential of cola drinks, however, the sweetener also has some influence. Key words:Tooth erosion, dental enamel, soft drinks, ph, sweetener.

[1]  M. Maltz,et al.  Dental erosion among South Brazilian adolescents: A 2.5‐year longitudinal study , 2018, Community dentistry and oral epidemiology.

[2]  M. Grindefjord,et al.  Dental erosion, prevalence and risk factors among a group of adolescents in Stockholm County , 2018, European Archives of Paediatric Dentistry.

[3]  P. Djekic,et al.  Erosive Effect of Different Soft Drinks on Enamel Surface in vitro: Application of Stylus Profilometry , 2015, Medical Principles and Practice.

[4]  M. Huysmans,et al.  Estimated prevalence of erosive tooth wear in permanent teeth of children and adolescents: an epidemiological systematic review and meta-regression analysis. , 2015, Journal of dentistry.

[5]  Martin Addy,et al.  The interactions between attrition, abrasion and erosion in tooth wear. , 2014, Monographs in oral science.

[6]  P. Benjakul,et al.  Association of dental enamel loss with the pH and titratable acidity of beverages , 2011 .

[7]  A. Lussi,et al.  Analysis of the erosive effect of different dietary substances and medications , 2011, British Journal of Nutrition.

[8]  A. Lussi,et al.  Screening and Prediction of Erosive Potential , 2011, Caries Research.

[9]  L. Tenuta,et al.  Initial Erosion Models , 2011, Caries Research.

[10]  D T Zero,et al.  Methodology and Models in Erosion Research: Discussion and Conclusions , 2011, Caries Research.

[11]  A. Lussi,et al.  Dental Erosion – An Overview with Emphasis on Chemical and Histopathological Aspects , 2011, Caries Research.

[12]  H. P. Chew,et al.  Clinical Studies of Dental Erosion and Erosive Wear , 2011, Caries Research.

[13]  N. Schlueter,et al.  Methods for the Measurement and Characterization of Erosion in Enamel and Dentine , 2011, Caries Research.

[14]  M. Buzalaf,et al.  An in situ/ex vivo comparison of the ability of regular and light colas to induce enamel wear when erosion is combined with abrasion. , 2011, Quintessence international.

[15]  M. Buzalaf,et al.  Effect of a single application of TiF(4) and NaF varnishes and solutions on dentin erosion in vitro. , 2010, Journal of dentistry.

[16]  A. Johansson,et al.  Dental erosion and soft drink consumption in Swedish children and adolescents and the development of a simplified erosion partial recording system. , 2010, Swedish dental journal.

[17]  M. Buzalaf,et al.  Light cola drink is less erosive than the regular one: an in situ/ex vivo study. , 2009, Journal of dentistry.

[18]  D. Zero,et al.  Analysis of the erosive potential of calcium-containing acidic beverages. , 2008, European journal of oral sciences.

[19]  P. Nihill,et al.  Commercial soft drinks: pH and in vitro dissolution of enamel. , 2007, General dentistry.

[20]  A. Bardow,et al.  Immediate Erosive Potential of Cola Drinks and Orange Juices , 2006, Journal of dental research.

[21]  M. Addy,et al.  Interaction between attrition,abrasion and erosion in tooth wear. , 2006, Monographs in oral science.

[22]  A. Lussi,et al.  Chemical factors. , 2006, Monographs in oral science.

[23]  T. Attin,et al.  Erosive effects of different acids on bovine enamel: release of calcium and phosphate in vitro. , 2005, Archives of oral biology.

[24]  C. Dugmore,et al.  Tooth wear: The progression of tooth erosion in a cohort of adolescents of mixed ethnicity , 2004, British Dental Journal.

[25]  S. Creanor,et al.  The pH and titratable acidity of a range of diluting drinks and their potential effect on dental erosion. , 2002, Journal of dentistry.

[26]  C. Ganss,et al.  Dental erosion in children and adolescents--a cross-sectional and longitudinal investigation using study models. , 2001, Community dentistry and oral epidemiology.

[27]  B. Nyvad,et al.  Enamel Erosion by Some Soft Drinks and Orange Juices Relative to Their pH, Buffering Effect and Contents of Calcium Phosphate , 1998, Caries Research.

[28]  A. Lussi,et al.  Prediction of the erosive potential of some beverages. , 1995, Caries research.

[29]  E. Hellwig,et al.  Fluoride taken up by plaque, by the underlying enamel and by clean enamel from three fluoride compounds in vitro. , 1982, Caries research.

[30]  W. B. Davis,et al.  The effect of abrasion on enamel and dentine and exposure to dietary acid , 1980, British Dental Journal.