Platelet Function in Platelet Concentrates and in Whole Blood

Platelet function was studied in CPD whole blood stored at 4°C for one and three days and in platelet concentrates stored at room temperature for the same periods of time. Comparisons were made of platelet shape, nucleotide content, β-thromboglobulin (βTG) liberated during storage, and platelet aggregation in response to ADP, collagen, sodium arachidonate and ristocetin. It was found that in whole blood the shape of the platelets was less discoid than in platelet concentrates. However, platelet aggregation in response to ADP, collagen, and sodium arachidonate was preserved better in whole blood than in platelet concentrates. Platelet nucleotides were the same in whole blood as in platelet concentrates, but the plasma levels of βTG were less in whole blood. The results show that as judged by aggregation, βTG release and nucleotide content, platelets from whole blood were at least as functional as those from platelet concentrates. However, platelets from whole blood had lost their discoid shape, which suggests that they would have a short survival in the circulation.

[1]  E. Duncan,et al.  Storage of platelets for tests of platelet function: comparison of two methods of pH control. , 1985, Thrombosis research.

[2]  J. Lloyd,et al.  Storage of platelets for tests of platelet function: effects of pH on platelet aggregation and liberation of beta-thromboglobulin. , 1983, Thrombosis research.

[3]  W. J. Russell,et al.  Platelet Counts in Stored Donor Blood , 1982, Anaesthesia and intensive care.

[4]  A. Sturk,et al.  The effect of storage on platelet morphology , 1982, Transfusion.

[5]  J. Lloyd,et al.  Effect of EDTA and PGE1 on beta-thromboglobulin liberation from platelets. , 1982, Thrombosis research.

[6]  E. Snyder.  Release of β‐Thromboglobulin during Storage of Platelet Concentrates , 1981 .

[7]  M. Mcgill.  Correlations of Platelet Survival and Function with the Results of Two in vitro Tests , 1981, Vox sanguinis.

[8]  J. Mustard,et al.  In vitro Methods and the Study of Platelet Mechanisms , 1981, Vox sanguinis.

[9]  S. Slichter.  In vitro Measurements of Platelet Concentrates Stored at 4 and 22°C: Correlation with Posttransfusion Platelet Viability and Function , 1981, Vox sanguinis.

[10]  L. Harker,et al.  Preparation and Storage of Platelet Concentrates , 2003, Transfusion.

[11]  L. Harker,et al.  Preparation and Storage of Platelet Concentrates: II. STORAGE VARIABLES INFLUENCING PLATELET VIABILITY AND FUNCTION , 1976, British journal of haematology.

[12]  C. Ludlam,et al.  Studies on liberation of beta-thromboglobulin from human platelets in vitro. , 1976, British journal of haematology.

[13]  R. Aster,et al.  Studies of Platelet Concentrates Stored at 22 C and 4 C , 1973, Transfusion.

[14]  C. Valeri,et al.  Hemostatic effectiveness of platelets stored at 22 degrees C. , 1971, The New England journal of medicine.

[15]  G. Roberts,et al.  The release of nucleotides, 5‐hydroxytryptamine and enzymes from human blood platelets during aggregation , 1968, The Journal of physiology.

[16]  H. Holmsen,et al.  Microdetermination of adenosine diphosphate and adenosine triphosphate in plasma with firefly luciferase system. , 1966, Analytical biochemistry.

[17]  F. Kissmeyer-Nielsen,et al.  Platelets in blood stored in untreated and siliconed glass bottles and plastic bags , 1961, Journal of clinical pathology.

[18]  W. Dameshek,et al.  The viability of stored human platelets. , 1960, Blood.