论文信息 - Strand Ligation in a Double-stranded DNA by T4 RNA Ligase

Strand Ligation in a Double-stranded DNA by T4 RNA Ligase

T4 RNA ligase was shown to ligate two DNA strands contained in a double helix with a high efficiency. Experiments on substrate and temperature dependence of this reaction supported that single-stranded ends clamped by a double-stranded region are necessary for this reaction. This finding is widely useful for DNA joining.

Y. Husimi | Y. Kinoshita | K. Nishigaki

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