High density, high radiance μLED matrix for optogenetic retinal prostheses and planar neural stimulation

Optical neuron stimulation arrays are important for both <italic>in-vitro</italic> biology and retinal prosthetic biomedical applications. Hence, in this work, we present an 8100 pixel high radiance photonic stimulator. The chip module vertically combines custom made gallium nitride <inline-formula><tex-math notation="LaTeX">$\mu$</tex-math> </inline-formula>LEDs with a CMOS application specific integrated circuit. This is designed with active pixels to ensure random access and to allow continuous illumination of all required pixels. The <inline-formula> <tex-math notation="LaTeX">$\mu$</tex-math></inline-formula>LEDs have been assembled on the chip using a solder ball flip-chip bonding technique which has allowed for reliable and repeatable manufacture. We have evaluated the performance of the matrix by measuring the different factors including the static, dynamic power consumption, the illumination, and the current consumption by each LED. We show that the power consumption is within a range suitable for portable use. Finally, the thermal behavior of the matrix is monitored and the matrix proved to be thermally stable.

[1]  Steve Marschner,et al.  Perceptually based tone mapping of high dynamic range image streams , 2005, EGSR '05.

[2]  Joachim Piprek,et al.  How to decide between competing efficiency droop models for GaN-based light-emitting diodes , 2015 .

[3]  Ruslana Shulyzki,et al.  320-Channel Active Probe for High-Resolution Neuromonitoring and Responsive Neurostimulation , 2015, IEEE Transactions on Biomedical Circuits and Systems.

[4]  Christofer Toumazou,et al.  A CMOS image sensor with light-controlled oscillating pixels for an investigative optobionic retinal prosthesis system , 2009, Microelectron. J..

[5]  B Guilhabert,et al.  Individually-addressable flip-chip AlInGaN micropixelated light emitting diode arrays with high continuous and nanosecond output power. , 2008, Optics express.

[6]  D. Ruderman,et al.  Statistics of cone responses to natural images: implications for visual coding , 1998 .

[7]  Alexandre Yakovlev,et al.  Optogenetics in Silicon: A Neural Processor for Predicting Optically Active Neural Networks , 2017, IEEE Transactions on Biomedical Circuits and Systems.

[8]  Hung Cao,et al.  An Integrated μLED Optrode for Optogenetic Stimulation and Electrical Recording , 2013, IEEE Transactions on Biomedical Engineering.

[9]  Karim Abdelhalim,et al.  The 128-Channel Fully Differential Digital Integrated Neural Recording and Stimulation Interface , 2010, IEEE Transactions on Biomedical Circuits and Systems.

[10]  K. Nikolic,et al.  Optoelectronic microarrays for retinal prosthesis , 2009, 2009 IEEE Biomedical Circuits and Systems Conference.

[11]  Walid I Al-Atabany,et al.  Designing and testing scene enhancement algorithms for patients with retina degenerative disorders , 2010, Biomedical engineering online.

[12]  K. Mathieson,et al.  Thermal and optical characterization of micro-LED probes for in vivo optogenetic neural stimulation. , 2013, Optics letters.

[13]  Ahmed Soltan,et al.  An 8100 pixel optoelectronic array for optogenetic retinal prosthesis , 2014, 2014 IEEE Biomedical Circuits and Systems Conference (BioCAS) Proceedings.

[14]  E. Bamberg,et al.  Channelrhodopsin-2, a directly light-gated cation-selective membrane channel , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[15]  A. Y. Chow,et al.  Subretinal electrical stimulation of the rabbit retina , 1997, Neuroscience Letters.

[16]  Pleun Maaskant,et al.  Development of optics with micro-LED arrays for improved opto-electronic neural stimulation , 2013, Photonics West - Biomedical Optics.

[17]  I. Underwood,et al.  Active-Matrix GaN Micro Light-Emitting Diode Display With Unprecedented Brightness , 2015, IEEE Transactions on Electron Devices.

[18]  Ava K. Bittner,et al.  The artificial silicon retina in retinitis pigmentosa patients (an American Ophthalmological Association thesis). , 2010, Transactions of the American Ophthalmological Society.

[19]  Wenliang Wang,et al.  Highly-efficient GaN-based light-emitting diode wafers on La0.3Sr1.7AlTaO6 substrates , 2015, Scientific Reports.

[20]  B. Wilhelm,et al.  Subretinal Visual Implant Alpha IMS – Clinical trial interim report , 2015, Vision Research.

[21]  Christofer Toumazou,et al.  Modeling Study of the Light Stimulation of a Neuron Cell With Channelrhodopsin-2 Mutants , 2011, IEEE Transactions on Biomedical Engineering.

[22]  Yun Miao,et al.  Optical Characterization of Tissue Phantoms Using a Silicon Integrated fdNIRS System on Chip , 2017, IEEE Transactions on Biomedical Circuits and Systems.

[23]  O. Paul,et al.  GaN-based micro-LED arrays on flexible substrates for optical cochlear implants , 2014 .

[24]  A. Sher,et al.  Photovoltaic Retinal Prosthesis with High Pixel Density , 2012, Nature Photonics.

[25]  A. Dizhoor,et al.  Ectopic Expression of a Microbial-Type Rhodopsin Restores Visual Responses in Mice with Photoreceptor Degeneration , 2006, Neuron.

[26]  Karl Deisseroth,et al.  Genetic Reactivation of Cone Photoreceptors Restores Visual Responses in Retinitis Pigmentosa , 2010, Science.

[27]  Patrick Degenaar,et al.  Optobionic vision—a new genetically enhanced light on retinal prosthesis , 2009, Journal of neural engineering.

[28]  Pleun Maaskant,et al.  High-Speed Substrate-Emitting Micro-Light-Emitting Diodes for Applications Requiring High Radiance , 2013 .

[29]  Patrick Degenaar,et al.  A New Individually Addressable Micro-LED Array for Photogenetic Neural Stimulation , 2010, IEEE Transactions on Biomedical Circuits and Systems.

[30]  Douglas S Kim,et al.  Light-activated channels targeted to ON bipolar cells restore visual function in retinal degeneration , 2008, Nature Neuroscience.

[31]  F. Dehkhoda,et al.  Smart optrode for neural stimulation and sensing , 2015, 2015 IEEE SENSORS.

[32]  Karol Myszkowski,et al.  A model of local adaptation , 2015, ACM Trans. Graph..

[33]  Peter Enoksson,et al.  Micromachined electrodes for biopotential measurements , 2001 .

[34]  Man-Kay Law,et al.  A Single-Chip Solar Energy Harvesting IC Using Integrated Photodiodes for Biomedical Implant Applications , 2017, IEEE Transactions on Biomedical Circuits and Systems.

[35]  Yongjian Sun,et al.  Fabrication, characterization and applications of flexible vertical InGaN micro-light emitting diode arrays. , 2016, Optics express.

[36]  F. Baldini,et al.  Biomedical sensors using optical fibres , 1996 .

[37]  Jessy D. Dorn,et al.  Interim results from the international trial of Second Sight's visual prosthesis. , 2012, Ophthalmology.

[38]  Andreas Hierlemann,et al.  Impedance characterization and modeling of electrodes for biomedical applications , 2005, IEEE Transactions on Biomedical Engineering.

[39]  Patrick Degenaar,et al.  A Processing Platform for Optoelectronic/Optogenetic Retinal Prosthesis , 2013, IEEE Transactions on Biomedical Engineering.

[40]  Thomas Guenther,et al.  Bionic vision: system architectures – a review , 2012, Expert review of medical devices.

[41]  Inbar Brosh,et al.  Holographic optogenetic stimulation of patterned neuronal activity for vision restoration , 2013, Nature Communications.

[42]  Simon Malpas,et al.  Pulse-Width Modulation of Optogenetic Photo-Stimulation Intensity for Application to Full-Implantable Light Sources , 2017, IEEE Transactions on Biomedical Circuits and Systems.

[43]  Sylvie Renaud,et al.  An Embedded Deep Brain Stimulator for Biphasic Chronic Experiments in Freely Moving Rodents , 2016, IEEE Transactions on Biomedical Circuits and Systems.

[44]  Maysam Ghovanloo,et al.  Design, fabrication, and packaging of an integrated, wirelessly-powered optrode array for optogenetics application , 2015, Front. Syst. Neurosci..

[45]  M. Dawson,et al.  High-speed GaN micro-LED arrays for data communications , 2012, 2012 14th International Conference on Transparent Optical Networks (ICTON).

[46]  J. L. Stone,et al.  Morphometric analysis of macular photoreceptors and ganglion cells in retinas with retinitis pigmentosa. , 1992, Archives of ophthalmology.

[47]  N Farah,et al.  Design and characteristics of holographic neural photo-stimulation systems , 2009, Journal of neural engineering.

[48]  K. Mathieson,et al.  Optogenetic activation of neocortical neurons in vivo with a sapphire-based micro-scale LED probe , 2015, Front. Neural Circuits.

[49]  Hongxing Jiang,et al.  III-nitride micro-emitter arrays: development and applications , 2008 .