The release of genetically modified crops into the environment. Part II. Overview of ecological risk assessment.

Despite numerous future promises, there is a multitude of concerns about the impact of GM crops on the environment. Key issues in the environmental assessment of GM crops are putative invasiveness, vertical or horizontal gene flow, other ecological impacts, effects on biodiversity and the impact of presence of GM material in other products. These are all highly interdisciplinary and complex issues. A crucial component for a proper assessment is defining the appropriate baseline for comparison and decision. For GM crops, the best and most appropriately defined reference point is the impact of plants developed by traditional breeding. The latter is an integral and accepted part of agriculture. In many instances, the putative impacts identified for GM crops are very similar to the impacts of new cultivars derived from traditional breeding. When assessing GM crops relative to existing cultivars, the increased knowledge base underpinning the development of GM crops will provide greater confidence in the assurances plant science can give on the risks of releasing such crops.

[1]  P. Dale The release of transgenic plants into agriculture , 1993, The Journal of Agricultural Science.

[2]  J. Pleasants,et al.  Temporal and spatial overlap between monarch larvae and corn pollen , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[3]  C. R. Linder POTENTIAL PERSISTENCE OF TRANSGENES: SEED PERFORMANCE OF TRANSGENIC CANOLA AND WILD × CANOLA HYBRIDS , 1998 .

[4]  N. W. Simmonds,et al.  Principles of crop improvement , 1979 .

[5]  Paul Slovic,et al.  Perceived risk, trust, and democracy , 1993 .

[6]  W. Nentwig,et al.  Decomposition of transgenic Bacillus thuringiensis maize by microorganisms and woodlice Porcellio scaber (Crustacea: Isopoda). , 2000 .

[7]  M. Witty,et al.  Integration of multiple repeats of geminiviral DNA into the nuclear genome of tobacco during evolution. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[8]  T. Franklin,et al.  Resistance to antimicrobial drugs , 1989 .

[9]  M. Williamson,et al.  Invaders, weeds and the risk from genetically manipulated organisms , 1993, Experientia.

[10]  M. Chrispeels Biotechnology and the poor. , 2000, Plant physiology.

[11]  C. R. Linder,et al.  Assessing the risks of transgene escape through time and crop‐wild hybrid persistence , 1994 .

[12]  S. Salzberg,et al.  Sequence and analysis of the Arabidopsis genome. , 2001, Current opinion in plant biology.

[13]  J. S. Heslop-Harrison,et al.  Integration of banana streak badnavirus into the Musa genome: molecular and cytogenetic evidence. , 1999, Virology.

[14]  P. Simonet,et al.  Horizontal gene transfers in the environment: natural transformation as a putative process for gene transfers between transgenic plants and microorganisms. , 1999, Research in microbiology.

[15]  A. Bostrom Risk Perception: “Experts” vs. “Lay People” , 1997 .

[16]  J A Eisen,et al.  Microbial Genes in the Human Genome: Lateral Transfer or Gene Loss? , 2001, Science.

[17]  H. Vries,et al.  Species and Varieties, Their Origin by Mutation , 1905 .

[18]  M. T. Johnson Interaction of Resistant Plants and Wasp Parasitoids of Tobacco Budworm (Lepidoptera: Noctuidae) , 1997 .

[19]  Jan-Peter Nap,et al.  The release of genetically modified crops into the environment. Part I. Overview of current status and regulations. , 2003, The Plant journal : for cell and molecular biology.

[20]  Jules Pretty,et al.  Regenerating Agriculture: Policies and Practice for Sustainability and Self-Reliance , 1995 .

[21]  A. McHughen Pandora's Picnic Basket: The Potential and Hazards of Genetically Modified Foods , 2000 .

[22]  M. Pham-Delègue,et al.  Effects of Proteinase Inhibitor Ingestion on Survival, Learning Abilities and Digestive Proteinases of the Honeybee , 1998, Transgenic Research.

[23]  The natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in the recipient cells. , 2001, FEMS microbiology letters.

[24]  M Dröge,et al.  Horizontal gene transfer as a biosafety issue: a natural phenomenon of public concern. , 1998, Journal of biotechnology.

[25]  G. Poppy,et al.  Interactions between insect tolerant genetically modified plants and natural enemies , 1999 .

[26]  B. Groombridge Global biodiversity: status of the earth's living resources. , 1992 .

[27]  P. Dale The impact of hybrids between genetically modified crop plants and their related species: general considerations , 1994 .

[28]  N. Jensen Plant Breeding Methodology , 1988 .

[29]  E. Rosa,et al.  Towards a more sustainable agriculture system: The effect of glucosinolates on the control of soil-borne diseases , 1999 .

[30]  I. Rigamonti,et al.  Effects of Bt corn on Rhopalosiphum padi L. (Rhynchota Aphididae) and on its predator Chrysoperla carnea Stephen (Neuroptera Chrysopidae) , 1998 .

[31]  G. Londo Botanical files on Lettuce (Lactuca sativa) - on the chance for gene flow between wild and cultivated Lettuce (Lactuca sativa L., including L. serriola L., Compositae) and the generalized implications for risk-assessments on genetically modified plants , 1964 .

[32]  W. Wackernagel,et al.  Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation , 1998, Molecular and General Genetics MGG.

[33]  F. Gould Sustainability of transgenic insecticidal cultivars: integrating pest genetics and ecology. , 1998, Annual review of entomology.

[34]  Catherine L. Moyes,et al.  ORGANIC FARMING AND GENE TRANSFER FROM GENETICALLY MODIFIED CROPS , 1999 .

[35]  H. Leung,et al.  Pathogen fitness penalty as a predictor of durability of disease resistance genes. , 2001, Annual review of phytopathology.

[36]  Martin Jenkins,et al.  Global biodiversity: earth's living resources in the 21st century. , 2000 .

[37]  R. O'Neill,et al.  The value of the world's ecosystem services and natural capital , 1997, Nature.

[38]  Division on Earth Field Testing Genetically Modified Organisms: Framework for Decisions , 1989 .

[39]  N. Ellstrand,et al.  When transgenes wander, should we worry? , 2001, Plant physiology.

[40]  H. G. Baker,et al.  The Evolution of Weeds , 1974 .

[41]  H Torgersen,et al.  Risk assessment of conventional crop plants in analogy to transgenic plants , 1998, Environmental science and pollution research international.

[42]  M. Williamson,et al.  A survey of differing views of weed classification: implications for regulation of introductions , 1992 .

[43]  A. Hilbeck,et al.  Prey‐mediated effects of Cry1Ab toxin and protoxin and Cry2A protoxin on the predator Chrysoperla carnea , 1999 .

[44]  M. Williamson Community response to transgenic plant release: predictions from British experience of invasive plants and feral crop plants , 1994 .

[45]  Bryan E. Denham Against the Gods: The Remarkable Story of Risk , 1997 .

[46]  D. Renz,et al.  On the fate of orally ingested foreign DNA in mice: chromosomal association and placental transmission to the fetus , 1998, Molecular and General Genetics MGG.

[47]  H. G. Baker,et al.  Characteristics and modes of origin of weeds. , 1965 .

[48]  R. Leemans,et al.  Biodiversity and Global Climate Change , 1992 .

[49]  J. P. Grime Biodiversity and Ecosystem Function: The Debate Deepens , 1997, Science.

[50]  C. Palm,et al.  Decomposition of Genetically Engineered Tobacco Under Field Conditions: Persistence of the Proteinase Inhibitor I Product and Effects on Soil Microbial Respiration and Protozoa, Nematode and Microarthropod Populations , 1997 .

[51]  J. Paul,et al.  Turnover of Extracellular DNA in Eutrophic and Oligotrophic Freshwater Environments of Southwest Florida , 1989, Applied and environmental microbiology.

[52]  S. Kaplan,et al.  On The Quantitative Definition of Risk , 1981 .

[53]  P. V. Haastert,et al.  Genomics: Genes lost during evolution , 2001, Nature.

[54]  J. Falck-Zepeda,et al.  Cotton GMO Adoption and Private Profitability , 2001 .

[55]  L. Lacey,et al.  Comparative efficacy of Beauveria bassiana, Bacillus thuringiensis, and aldicarb for control of Colorado potato beetle in an irrigated desert agroecosystem and their effects on biodiversity , 1999 .

[56]  M. Tepfer Viral Genes and Transgenic Plants , 1993, Bio/Technology.

[57]  D. Saxena,et al.  Bt toxin uptake from soil by plants , 2001, Nature Biotechnology.

[58]  W. Brandenburg,et al.  Botanical Files. A study of the real chances for spontaneous gene flow from cultivated plants to the wild flora of the Netherlands , 1992 .

[59]  René von Schomberg,et al.  Regulating agricultural biotechnology in Europe: harmonisation difficulties, opportunities, dilemmas , 1996 .

[60]  Michael J. Stanhope,et al.  Phylogenetic analyses do not support horizontal gene transfers from bacteria to vertebrates , 2001, Nature.

[61]  J. Nap,et al.  A transgene-centered approach to the biosafety of transgenic phosphinothricin-tolerant plants , 1998, Molecular Breeding.

[62]  M. Crawley The population biology of invaders , 1986 .

[63]  Julie Babinard,et al.  The Domestic and Regional Regulatory Environment , 2001 .

[64]  R. Pukall,et al.  Prevalence of nptII and Tn5 in kanamycin‐resistant bacteria from different environments , 1993 .

[65]  M. Berenbaum,et al.  Effects of exposure to event 176 Bacillus thuringiensis corn pollen on monarch and black swallowtail caterpillars under field conditions , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[66]  H. Jørgensen,et al.  Tri‐trophic effect on predator feeding: consumption by the carabid Harpalus affinis of Heliothis armigera caterpillars fed on proteinase inhibitor‐containing diet , 1999 .

[67]  J. White The Commercial Use of Biodiversity: Access to Genetic Resources and Benefit-sharing , 2001 .

[68]  R. Johnson A critical analysis of durable resistance , 1984 .

[69]  G. Gaskell,et al.  Worlds apart? The reception of genetically modified foods in Europe and the U.S. , 1999, Science.

[70]  C. Kunin,et al.  Resistance to Antimicrobial DrugsA Worldwide Calamity , 1993, Annals of Internal Medicine.

[71]  Claire Marris,et al.  Public Perceptions of Agricultural Biotechnologies in Europe: Report of the PABE project funded by the European Commission, DG Research (contract number: FAIR CT98-3844 (DG12 - SSMI) , 2001 .

[72]  A. Cropper Convention on Biological Diversity , 1993, Environmental Conservation.

[73]  A. Hilbeck,et al.  Effects of transgenic Bacillus thuringiensis corn-fed prey on mortality and development time of immature Chrysoperla cornea (Neuroptera: Chrysopidae) , 1998 .

[74]  J. Kolmer Genetics of resistance to wheat leaf rust. , 1996, Annual review of phytopathology.

[75]  B. Law Genetically Modified Crops: The Ethical and Social Issues , 2001 .

[76]  W. Landis,et al.  Dynamics of Horizontal Gene Transfer and the Ecological Risk Assessment of Genetically Engineered Organisms , 2000 .

[77]  J. Bennett Biodiversity and pest management in agroecosystems , 2005 .

[78]  M. Matzke,et al.  Integrated pararetroviral sequences define a unique class of dispersed repetitive DNA in plants. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[79]  M. G. Lorenz,et al.  DNA Binding to Various Clay Minerals and Retarded Enzymatic Degradation of DNA in a Sand/Clay Microcosm , 1992 .

[80]  A. J. Conner,et al.  Plant breeding and seed marketing options for the introduction of transgenic insect‐resistant crops , 1994 .

[81]  B. Wynne,et al.  Public Perceptions of Agricultural Biotechnologies in Europe (PABE) , 2001 .

[82]  A. C. Zeven,et al.  Dictionary of cultivated plants and their regions of diversity: excluding most ornamentals, forest trees and lower plants. , 1982 .

[83]  J. Parlevliet Durability of resistance against fungal, bacterial and viral pathogens; present situation , 2002, Euphytica.

[84]  Ray F. Smith Cast (Council for Agricultural Science and Technology) , 1976 .

[85]  P. Reich,et al.  The Influence of Functional Diversity and Composition on Ecosystem Processes , 1997 .

[86]  L. Firbank,et al.  Genetically Modified Crops and Farmland Biodiversity , 2000, Science.

[87]  A. Birch,et al.  Tri-trophic interactions involving pest aphids, predatory 2-spot ladybirds and transgenic potatoes expressing snowdrop lectin for aphid resistance , 2004, Molecular Breeding.

[88]  I. Serageldin World Poverty and Hunger--the Challenge for Science , 2002, Science.

[89]  A. Trewavas,et al.  Is opposition to GM crops science or politics? , 2001, EMBO reports.

[90]  C. Golz,et al.  Foreign DNA sequences are received by a wild-type strain of Aspergillus niger after co-culture with transgenic higher plants , 1994, Current Genetics.

[91]  Henry Daniell,et al.  Containment of herbicide resistance through genetic engineering of the chloroplast genome , 1998, Nature Biotechnology.

[92]  L. Malone,et al.  Effects of transgene products on honey bees (Apis mellifera) and bumblebees (Bombus sp.) , 2001 .

[93]  Gerald C. Nelson Genetically Modified Organisms in Agriculture: Economics and Politics , 2001 .

[94]  P. Courvalin Transfer of antibiotic resistance genes between gram-positive and gram-negative bacteria , 1994, Antimicrobial Agents and Chemotherapy.

[95]  H. V. Gelder The Netherlands , 2004, Constitutions of Europe (2 vols.).

[96]  E. J. Evans,et al.  Contamination of edible double-low oilseed rape crops via pollen transfer from high erucic cultivars , 1998 .

[97]  P. Rieder,et al.  Biodiversity, Biotechnology, and Institutions Among Crops:: Situation and Outlook , 1995 .

[98]  Stanley P. Johnson,et al.  The Earth Summit : the United Nations Conference on Environment and Development (UNCED) , 1995 .

[99]  I. Goklany Applying the Precautionary Principle to Genetically Modified Crops , 2000 .

[100]  Quantitative Risk Assessment Breeds Fear , 1999 .

[101]  David W. Hopkins,et al.  Decomposition in soil of tobacco plants with genetic modifications to lignin biosynthesis , 2001 .

[102]  P. Ehrlich,et al.  Biodiversity Studies: Science and Policy , 1991, Science.

[103]  A. Hilbeck,et al.  Toxicity of Bacillus thuringiensis Cry1Ab toxin to the predator Chrysoperla carnea (Neuroptera: Chrysopidae) , 1998 .

[104]  I. Potrykus,et al.  “Horizontal” Gene Transfer from a Transgenic Potato Line to a Bacterial Pathogen (Erwinia chrysanthemi) Occurs—if at All—at an Extremely Low Frequency , 1995, Bio/Technology.

[105]  M. Crawley,et al.  Ecology of transgenic oilseed rape in natural habitats , 1993, Nature.

[106]  G. Edwards,et al.  Transgenic DNA introgressed into traditional maize landraces in Oaxaca , Mexico , 2001 .

[107]  D. Stefanović,et al.  Effects of four protease inhibitors on the survival of worker bumblebees, Bombus terrestris L. , 2000 .

[108]  Scott Rozelle,et al.  Plant Biotechnology in China , 2002, Science.

[109]  P. Oger,et al.  Genetically engineered plants producing opines alter their biological environment , 1997, Nature Biotechnology.

[110]  Charles Darwin,et al.  The Effects of Cross and Self Fertilisation in the Vegetable Kingdom , 1972 .

[111]  L. Orgel,et al.  Phylogenetic Classification and the Universal Tree , 1999 .

[112]  J. Heritage,et al.  Survival of free DNA encoding antibiotic resistance from transgenic maize and the transformation activity of DNA in ovine saliva, ovine rumen fluid and silage effluent. , 2000, FEMS microbiology letters.

[113]  P. Kareiva Transgenic plants on trial , 1993, Nature.

[114]  Theroyalsociety Transgenic plants and world agriculture , 2000 .

[115]  S. R. Jammalamadaka,et al.  Against the Gods: The Remarkable Story of Risk , 1999 .

[116]  A. J. Conner Genetically engineered crops , 1997 .

[117]  R. Phipps,et al.  Environmental benefits of genetically modified crops: Global and European perspectives on their ability to reduce pesticide use , 2002 .

[118]  F. V. Tongeren,et al.  Potential impacts of genetic use restriction technologies (GURTs) on agrobiodiversity and agricultural production systems , 2001 .

[119]  R. Fuchs,et al.  Safety Assessment of the Neomycin Phosphotransferase II (NPTII) Protein , 1993, Bio/Technology.

[120]  H. Kuiper,et al.  Assessment of the food safety issues related to genetically modified foods. , 2001, The Plant journal : for cell and molecular biology.

[121]  K. Leisinger,et al.  Six Billion and Counting: Population Growth and Food Security in the 21st Century , 2002 .

[122]  L. Simonsen Field testing genetically modified organisms: framework for decisions. National Research Council executive summary. , 1990, Recombinant DNA technical bulletin.

[123]  P. Kareiva,et al.  Assessing the risks of invasion for genetically engineered plants: acceptable evidence and reasonable doubt. , 1996 .

[124]  M. Whalon,et al.  Managing Insect Resistance to Bacillus thuringiensis Toxins , 1992, Science.

[125]  L. Watrud,et al.  A field study with genetically engineered alfalfa inoculated with recombinant Sinorhizobium meliloti: effects on the soil ecosystem , 1999 .

[126]  A. Pühler,et al.  Horizontal gene transfer among bacteria in terrestrial and aquatic habitats as assessed by microcosm and field studies , 1999, Biology and Fertility of Soils.

[127]  Paul Slovic,et al.  Trust, Emotion, Sex, Politics, and Science: Surveying the Risk-Assessment Battlefield , 1999 .

[128]  C. Davenport Species and Varieties, their Origin by Mutation , 1905 .

[129]  R. McIntosh,et al.  Anticipatory breeding for resistance to rust diseases in wheat. , 1997, Annual review of phytopathology.

[130]  G. Stotzky,et al.  Insecticidal activity and biodegradation of the toxin from bacillus thuringiensis subsp. kurstaki bound to humic acids from soil , 1998 .

[131]  Morris A. Levin,et al.  Risk Assessment in Genetic Engineering , 1991 .

[132]  W. Brandenburg,et al.  Interactions between wild and cultivated carrots (Daucus carota L.) in the Netherlands , 2004, Euphytica.

[133]  F. de la Cruz,et al.  Horizontal gene transfer and the origin of species: lessons from bacteria. , 2000, Trends in microbiology.

[134]  R. L. Stuckey,et al.  Spread, impact and control of purple loosestrife (Lythrum salicaria) in North American wetlands. , 1987 .

[135]  C. Kunin,et al.  Resistance to antimicrobial drugs--a worldwide calamity. , 1993, Annals of internal medicine.

[136]  P. Christou No Credible Scientific Evidence is Presented to Support Claims that Transgenic DNA was Introgressed into Traditional Maize Landraces in Oaxaca, Mexico , 2002, Transgenic Research.

[137]  B. Croft,et al.  Effects of Bacillus thuringiensis toxins in transgenic cotton and potato on Folsomia candida (Collembola: Isotomidae) and Oppia nitens (Acari: Orbatidae) , 1997 .

[138]  A. Purvis,et al.  Getting the measure of biodiversity , 2000, Nature.

[139]  Brian G. Wolff,et al.  Forecasting Agriculturally Driven Global Environmental Change , 2001, Science.

[140]  R. Huffman,et al.  Evaluation of insecticides for boll weevil control and impact on non-target arthropods on non-transgenic and transgenic B.t. cotton cultivars. , 1997 .

[141]  J. Eisen Horizontal gene transfer among microbial genomes: new insights from complete genome analysis. , 2000, Current opinion in genetics & development.

[142]  A. van Dommelen Coping with deliberate release: the limits of risk assessment. , 1996 .

[143]  K. Nielsen,et al.  Horizontal gene transfer from transgenic plants to terrestrial bacteria--a rare event? , 1998, FEMS microbiology reviews.

[144]  J. Beringer Releasing genetically modified organisms: will any harm outweigh any advantage? , 2000 .

[145]  K. Keeler Can Genetically Engineered Crops Become Weeds? , 1989, Nature Biotechnology.

[146]  J. Losey,et al.  Assessing the impact of Cry1Ab-expressing corn pollen on monarch butterfly larvae in field studies , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[147]  Stanley P. Johnson The Earth Summit , 1993 .

[148]  W. M. Robertson,et al.  Testing genetically engineered potato, producing the lectins GNA and Con A, on non‐target soil organisms and processes , 2000 .

[149]  J. Harlan Genetic Resources in Wild Relatives of Crops 1 , 1976 .

[150]  L. Levidow,et al.  Unsound science? Transatlantic regulatory disputes over GM crops. , 2000 .

[151]  Division on Earth,et al.  Environmental Effects of Transgenic Plants: The Scope and Adequacy of Regulation , 2002 .

[152]  C. R. Linder,et al.  Potential Persistence of Escaped Transgenes: Performance of Transgenic, Oil‐Modified Brassica Seeds and Seedlings , 1995 .

[153]  N. Jensen Intra‐varietal Diversification in Oat Breeding1 , 1952 .

[154]  Lisa J. Evered,et al.  Innumeracy: Mathematical Illiteracy and its Consequences. , 1990 .

[155]  J. Gatehouse,et al.  Snowdrop lectin (GNA) has no acute toxic effects on a beneficial insect predator, the 2-spot ladybird (Adalia bipunctata L.). , 2000, Journal of insect physiology.

[156]  M. G. Lorenz,et al.  Bacterial gene transfer by natural genetic transformation in the environment. , 1994, Microbiological reviews.

[157]  R. Downey Environmental Effects of Transgenic Plants , 2003 .

[158]  A. D. D. Species and Varieties: Their Origin by Mutation , 1907, Nature.

[159]  M. Williamson,et al.  Weed Probability Challenged , 1990, Bio/Technology.

[160]  J. Lawton,et al.  Does biodiversity matter? Evaluation the case for conserving species , 1996 .

[161]  G. Poppy,et al.  Parasitoid behaviour and Bt plants , 1999, Nature.

[162]  H. Mooney,et al.  Human Domination of Earth’s Ecosystems , 1997, Renewable Energy.

[163]  T. Malvar,et al.  Global variation in the genetic and biochemical basis of diamondback moth resistance to Bacillus thuringiensis. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[164]  P. Slovic Perception of risk. , 1987, Science.

[165]  APPLICATIONS OF BIOTECHNOLOGY TO CROPS : BENEFITS AND RISKS , 1999 .

[166]  J. Losey,et al.  Transgenic pollen harms monarch larvae , 1999, Nature.

[167]  Jonathan B. Wiener,et al.  Comparing precaution in the United States and Europe , 2002 .

[168]  P. Bakker,et al.  Influence of the production of antibacterial and antifungal proteins by transgenic plants on the saprophytic soil microflora , 1997 .

[169]  H. Ochman,et al.  Lateral gene transfer and the nature of bacterial innovation , 2000, Nature.

[170]  C. Palm,et al.  Changes in levels, species and DNA fingerprints of soil microorganisms associated with cotton expressing the Bacillus thuringiensis var. kurstaki endotoxin☆ , 1995 .

[171]  Richard L. Hellmich,et al.  Impact of Bt corn pollen on monarch butterfly populations: A risk assessment , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[172]  R. Hellmich,et al.  Monarch larvae sensitivity to Bacillus thuringiensis- purified proteins and pollen , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[173]  K. Smalla,et al.  Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer , 1999 .

[174]  Robert M. May,et al.  How Many Species Are There on Earth? , 1988, Science.

[175]  Richard L. Hellmich,et al.  Corn pollen deposition on milkweeds in and near cornfields , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[176]  J. Allan,et al.  Report of the Royal Commission on Genetic Modification. , 2001 .

[177]  J. Perry,et al.  Farm-scale evaluation of GM crops explained , 1999, Nature.

[178]  M. Crawley,et al.  Biotechnology: Transgenic crops in natural habitats , 2001, Nature.

[179]  G. S. Khush,et al.  Breeding Rice for Resistance to Pests , 1992 .

[180]  R. Ornduff,et al.  Crops and Man. , 1977 .

[181]  D. Renz,et al.  Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[182]  Doolittle Wf Phylogenetic Classification and the Universal Tree , 1999 .

[183]  H H Flor,et al.  Current Status of the Gene-For-Gene Concept , 1971 .

[184]  M. Pham-Delègue,et al.  Impact of Proteins Used in Plant Genetic Engineering: Toxicity and Behavioral Study in the Honeybee , 1997 .

[185]  J. Harlan,et al.  THE COMPILOSPECIES CONCEPT , 1963 .

[186]  K. Gaston,et al.  Biodiversity: An Introduction , 1998 .

[187]  David A. Andow,et al.  F2 screen for rare resistance alleles , 1998 .

[188]  D. Quist,et al.  Biodiversity (Communications arising (reply)): Suspect evidence of transgenic contamination/Maize transgene results in Mexico are artefacts (see editorial footnote) , 2002, Nature.

[189]  J. Rissler,et al.  Perils amidst the promise: ecological risks of transgenic crops in a global market , 1993 .

[190]  Helge Torgersen,et al.  Ecological impacts of traditional crop plants - a basis for the assessment of transgenic plants , 1997 .

[191]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[192]  B. Dagallier OECD schemes for the varietal certification of seed moving in international trade. , 2000 .

[193]  Travis R. Glare,et al.  Bacillus Thuringiensis: Biology, Ecology and Safety , 2000 .

[194]  R. McIntosh,et al.  Breeding wheat for resistance to biotic stresses , 2004, Euphytica.

[195]  J. Nap,et al.  A transgene-centered approach to the biosafety of transgenic crops: overview of selection and reporter genes , 1997 .

[196]  Honor C. Prentice,et al.  Gene Flow and Introgression from Domesticated Plants into Their Wild Relatives , 1999 .

[197]  M. Savka,et al.  Modification of rhizobacterial populations by engineering bacterium utilization of a novel plant-produced resource , 1997, Nature Biotechnology.

[198]  M. Yamaguchi,et al.  World Vegetables: Principles, Production and Nutritive Values , 1983 .

[199]  R. Anderson,et al.  The relationship between the volume of antimicrobial consumption in human communities and the frequency of resistance. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[200]  R. Freckleton,et al.  Predictions of biodiversity response to genetically modified herbicide-tolerant crops. , 2000, Science.