Repetitive element sequence‐based PCR for species and strain discrimination in the genus Listeria

Repetitive element sequencebased PCR (rep‐PCR) was used to generate DNA fingerprints for Listeria spp. Two primer sets (REP 1R‐I REP 2‐I and ERIC 1R ERIC 2) used in respectively REP‐and ERIC‐PCR revealed that bacteria of the genus Listeria possess short repetitive extragenic palindromic elements and enterobacterial repetitive intergenic consensus sequences. Specific band profiles obtained by ERIC‐PCR enabled the identification of Listeria species. With both REP‐and ERIC‐PCR the L. monocytogenes serotypes 1/2a, 1/2b, 1/2c, 3b and 4b could be clearly distinguished from each other. Within the serotype 1/2a, REP‐PCR showed a higher discriminative potential than ERIC‐PCR and a comparable discriminative potential as RAPD combining 3‐4 primers.

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