Radiation hybrid mapping: a somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes.

Radiation hybrid (RH) mapping, a somatic cell genetic technique, was developed as a general approach for constructing long-range maps of mammalian chromosomes. This statistical method depends on x-ray breakage of chromosomes to determine the distances between DNA markers, as well as their order on the chromosome. In addition, the method allows the relative likelihoods of alternative marker orders to be determined. The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. The map was confirmed by pulsed-field gel electrophoretic analysis. The results demonstrate the effectiveness of RH mapping for constructing high-resolution, contiguous maps of mammalian chromosomes.

[1]  Frank Stewart,et al.  June , 1890, The Hospital.

[2]  S. Goss,et al.  New method for mapping genes in human chromosomes , 1975, Nature.

[3]  D. Schwartz,et al.  Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis , 1984, Cell.

[4]  D. Patterson,et al.  Molecular Analysis of Chromosome 21 Using Somatic Cell Hybrids a , 1985, Annals of the New York Academy of Sciences.

[5]  G. D. Stewart,et al.  Cloned DNA probes regionally mapped to human chromosome 21 and their use in determining the origin of nondisjunction. , 1985, Nucleic acids research.

[6]  G. Landes,et al.  Isolation of polymorphic DNA segments from human chromosome 21. , 1985, Nucleic acids research.

[7]  O. Elroy-Stein,et al.  Molecular Structure and Expression of the Gene Locus on Chromosome 21 Encoding the Cu/Zn Superoxide Dismutase and Its Relevance to Down Syndrome a , 1985, Annals of the New York Academy of Sciences.

[8]  R. W. Davis,et al.  Separation of large DNA molecules by contour-clamped homogeneous electric fields. , 1986, Science.

[9]  A. Monaco,et al.  Isolation of candidate cDNAs for portions of the Duchenne muscular dystrophy gene , 1986, Nature.

[10]  A. Monaco,et al.  Cloning the gene for an inherited human disorder—chronic granulomatous disease—on the basis of its chromosomal location , 1986, Nature.

[11]  E. Jabs,et al.  Regional localization of DNA sequences on chromosome 21 using somatic cell hybrids. , 1986, American journal of human genetics.

[12]  M. Olson,et al.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. , 1986, Science.

[13]  T. Pryor.  The origin and structure of fungal disease resistance genes in plants , 1987 .

[14]  P. S. St George-Hyslop,et al.  Amyloid beta protein gene: cDNA, mRNA distribution, and genetic linkage near the Alzheimer locus. , 1987, Science.

[15]  Hans Lehrach,et al.  A large inverted duplication allows homologous recombination between chromosomes heterozygous for the proximal t complex inversion , 1987, Cell.

[16]  M. Daly,et al.  A genetic linkage map of the human genome , 1987, Cell.

[17]  D. Cox,et al.  Isolation and regional mapping of DNA sequences unique to human chromosome 21. , 1987, American journal of human genetics.

[18]  D. Patterson,et al.  Partial physical map of human chromosome 21 , 1988, Somatic cell and molecular genetics.

[19]  G. D. Stewart,et al.  Genetic linkage map of human chromosome 21. , 1988, Genomics.

[20]  R. Myers,et al.  Segregation of the Huntington disease region of human chromosome 4 in a somatic cell hybrid. , 1989, Genomics.

[21]  R. Myers,et al.  Isolation and field-inversion gel electrophoresis analysis of DNA markers located close to the Huntington disease gene. , 1989, Genomics.

[22]  Y. Nakamura,et al.  Twenty-eight loci form a continuous linkage map of markers for human chromosome 1. , 1989, Genomics.

[23]  L. Tsui,et al.  Erratum: Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNA , 1989, Science.

[24]  A. Pakstis,et al.  Report of the nomenclature committee and the 1989 catalog of mapped genes. , 1989, Cytogenetics and cell genetics.

[25]  P. Goodfellow,et al.  A method for generating hybrids containing nonselected fragments of human chromosomes. , 1989, Genomics.

[26]  A. Chakravarti,et al.  A genetic linkage map of 17 markers on human chromosome 21. , 1989, Genomics.

[27]  L. Tsui,et al.  Identification of the cystic fibrosis gene: chromosome walking and jumping. , 1989, Science.

[28]  S. Antonarakis,et al.  Molecular mechanism in the formation of a human ring chromosome 21. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[29]  G Hermanson,et al.  High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. , 1990, Science.

[30]  D. Housman,et al.  A panel of irradiation-reduced hybrids selectively retaining human chromosome 11p13: their structure and use to purify the WAGR gene complex. , 1990, Genomics.

[31]  P. Goodfellow,et al.  Generation of a panel of somatic cell hybrids containing unselected fragments of human chromosome 10 by X-ray irradiation and cell fusion: Application to isolating theMEN2A region in hybrid cells , 1990, Somatic cell and molecular genetics.

[32]  M. Owen,et al.  Physical mapping around the Alzheimer disease locus on the proximal long arm of chromosome 21. , 1990, American journal of human genetics.