OCCURRENCE OF AFLATOXIN M1 IN RAW MILK PRODUCED IN ARDEBIL OF IRAN

Contamination of milk and dairy products to aflatoxin M1 is a risk for human and it can be a public heath concern. The aim of this study was to evaluate the presence of aflatoxin M1 in raw milk samples produced in Ardebil City (Iran) by ELISA (Enzyme Linked Immuno Sorbent Assay) technique. 122 samples of raw milk were collected from milk collecting centers and dairy plants in the region and aflatoxin M1 contamination was detected in all of milk samples. The mean concentration of aflatoxin M1 was 40.01ng/L and 14.75 percent of the samples had higher levels than the maximum recommended limits by ISIRI, European Community and Codex Alimentarius. With a view of the fact that milk is used by all the age groups including infants and children in the city of Ardebil, it is necessary to apply an ideal recommended limit to minimize the health hazard from aflatoxin M1 contamination in milk. Application of Good Agricultural Practices and Good Veterinary Practices by agriculture and also the Hazard Analysis and Critical Control Points (HACCP) system as a draft code of practice for preharvest and postharvest control of dairy cow’s feed and in milk and dairy products processing is effective.

[1]  T. Phillips,et al.  Development and evaluation of a minicolumn assay for the detection of aflatoxin M1 in milk. , 1994, Journal of dairy science.

[2]  Mehran Moradi,et al.  Detection of aflatoxin M1 in raw and commercial pasteurized milk in Urmia, Iran. , 2007, Pakistan journal of biological sciences : PJBS.

[3]  M. Tajkarimi,et al.  Seasonal study of aflatoxin M1 contamination in milk in five regions in Iran. , 2007, International journal of food microbiology.

[4]  P. Krogh Mycotoxins in food , 1987 .

[5]  I. Ghanem,et al.  Aflatoxin M1 in raw, pasteurized and powdered milk available in the Syrian market , 2009 .

[6]  S. Dragacci,et al.  Use of immunoaffinity chromatography as a purification step for the determination of aflatoxin M1 in cheeses. , 1995, Food additives and contaminants.

[7]  B. Jannat,et al.  Presence of aflatoxin M1 in milk and infant milk products in Tehran, Iran , 2007 .

[8]  Ö. Küplülü,et al.  Detection of aflatoxin M1 in cheese samples by ELISA , 2004 .

[9]  A Prandini,et al.  On the occurrence of aflatoxin M1 in milk and dairy products. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[10]  J. Jacquet [Food microbiology]. , 1980, Annales de la nutrition et de l'alimentation.

[11]  F. Galvano,et al.  Detoxification methods of aflatoxins. A review , 1995 .

[12]  N. Bilandžić,et al.  Aflatoxin M1 in raw milk in Croatia , 2010 .

[13]  C. Kocabaş,et al.  Does systemic exposure to aflatoxin B1 cause allergic sensitization? , 2003, Allergy.

[14]  L. Smorenburg-van Middelkoop European Community (EC) , 2007 .

[15]  H. Alomirah,et al.  Levels of aflatoxin M1 in milk, cheese consumed in Kuwait and occurrence of total aflatoxin in local and imported animal feed , 2009 .

[16]  D. Kitchens Park Forest South , 1970 .

[17]  Ihsan Bakirci,et al.  A study on the occurrence of aflatoxin M1 in milk and milk products produced in Van province of Turkey , 2001 .

[18]  A. Khanafari,et al.  BIOCONTROL OF ASPERGILLUS FLAVUS AND AFLATOXIN B1 PRODUCTION IN CORN , 2007 .

[19]  M. Tajkarimi,et al.  Aflatoxin M1 contamination in winter and summer milk in 14 states in Iran , 2008 .

[20]  N. Botsoglou,et al.  Occurrence of aflatoxin M1 in raw and market milk commercialized in Greece , 2002, Food additives and contaminants.

[21]  M. A. Jonker,et al.  Current situation on regulations for mycotoxins , 2003 .

[22]  K. Naddafi,et al.  SURVEY OF PEANUT FUNGAL CONTAMINATION AND ITS RELATIONSHIP WITH AMBIENT CONDITIONS IN THE BAZAR OF ZANJAN , 2009 .

[23]  Premendra D. Dwivedi,et al.  Detection of Aflatoxin M1 contamination in milk and infant milk products from Indian markets by ELISA , 2004 .

[24]  I. Hussain,et al.  A study on contamination of aflatoxin M1 in raw milk in the Punjab province of Pakistan , 2008 .

[25]  A. Khosravi,et al.  Presence of Aflatoxin M1 in raw milk at cattle farms in Babol, Iran. , 2008, Pakistan journal of biological sciences : PJBS.

[26]  P. Markaki,et al.  Occurrence of aflatoxin M1 in commercial pasteurized milk determined with ELISA and HPLC. , 1997, Food additives and contaminants.

[27]  A. Kamkar,et al.  A study on the occurrence of aflatoxin M1 in raw milk produced in Sarab city of Iran , 2005 .

[28]  E. Creppy Update of survey, regulation and toxic effects of mycotoxins in Europe. , 2002, Toxicology letters.

[29]  Mohammad Hosein Movassagh Ghazani,et al.  Aflatoxin M1 contamination in pasteurized milk in Tabriz (northwest of Iran). , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[30]  A. Fallah,et al.  Determination of aflatoxin M1 levels in Iranian white and cream cheese. , 2009, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[31]  L. Bulacio,et al.  Presence of aflatoxin M1 in milk for human consumption in Argentina , 2003 .

[32]  M. Peraica,et al.  Toxic effects of mycotoxins in humans. , 1999, Bulletin of the World Health Organization.

[33]  L. Decastelli,et al.  Aflatoxins occurrence in milk and feed in Northern Italy during 2004–2005 , 2007 .