Time-dependent effects of fertilization on plant biomass in floating fens

Abstract A cross-over fertilization experiment was carried out in Dutch floating fens to investigate effects on biomass production in the same and the following years. In total 16 fertilizer treatments were applied, combining four treatments in 1999 with four treatments in 2000 (addition of 20 g.m−2 N, 5 g.m−2 P, both elements and unfertilized control). The above-ground biomass production of vascular plants was co-limited by N and P in both years. However, in plots that were only fertilized in 1999 the effects of individual nutrients differed between the two years: N-fertilization slightly increased the amount of biomass produced in the same year (1999), whereas P-fertilization did so in the following year (2000). Fertilizer applied in 1999 also influenced the effects of fertilizer applied in 2000. One year after N-fertilization vascular plant growth was still co-limited by N and P, but one year after P-fertilization, vascular plant growth was only limited by N. Bryophyte biomass responded weakly to fertilization. Nutrient concentrations in plant biomass, nutrient standing crops and measurements of N and P availability in the soil indicated that one year after fertilization, the N-fertilizer had mostly ‘disappeared’ from N-fertilized plots, whereas the availability of P remained markedly enhanced in P-fertilized plots. In addition, P-fertilization enhanced the uptake of N by plants the following year. The time-dependence of fertilizer effects was probably caused by (1) higher addition of P than of N relative to the requirements of plants; (2) longer retention of P than of N in the system; (3) positive effect of P-fertilization on the availability of N; (4) contrasting effects of N- and P-fertilization on nutrient losses by plants and/or on their responses to subsequent nutrient addition; (5) changing interactions between vascular plants and mosses (mainly Sphagnum spp.); (6) nutrient export through the repeated harvest of above-ground biomass. To determine which nutrient limits plant growth fertilization experiments should be short, avoiding that indirect effects of a non-limiting nutrient influence results. To indicate how changed nutrient supply will affect an ecosystem longer-term experiments are needed, so that indirect effects have time to develop and be detected. Nomenclature: Tutin et al. (1964–1993).

[1]  M. Wassen,et al.  Nutrient limitation along a productivity gradient in wet meadows , 2001, Plant and Soil.

[2]  I. V. Van Duren,et al.  Nutrient limitations in wet, drained and rewetted fen meadows: evaluation of methods and results , 2000, Plant and Soil.

[3]  J. Arnone.  Indices of plant N availability in an alpine grassland under elevated atmospheric CO2 , 1997, Plant and Soil.

[4]  D. Boeye,et al.  A local rich fen fed by calcareous seepage from an artificial river water infiltration system , 1996, Vegetatio.

[5]  R. Aerts,et al.  Interspecific and intraspecific differences in shoot and leaf lifespan of four Carex species which differ in maximum dry matter production , 1995, Oecologia.

[6]  H. Olff.  Effects of light and nutrient availability on dry matter and N allocation in six successional grassland species , 1992, Oecologia.

[7]  L. Aarssen,et al.  Resource manipulations in natural vegetation: a review , 1989, Vegetatio.

[8]  W. Oechel,et al.  The role of mosses in the phosphorus cycling of an Alaskan black spruce forest , 1987, Oecologia.

[9]  G. Shaver.  Mineral nutrition and leaf longevity in Ledum palustre: the role of individual nutrients and the timing of leaf mortality , 1983, Oecologia.

[10]  G. Shaver,et al.  Mineral nutrition and leaf longevity in an evergreen shrub, Ledum palustre ssp. decumbens , 1981, Oecologia.

[11]  O. Oenema,et al.  Nitrogen loss from grassland on peat soils through nitrous oxide production , 2004, Plant and Soil.

[12]  R. Aerts.  The effect of increased nutrient availability on leaf turnover and aboveground productivity of two evergreen ericaceous shrubs , 2004, Oecologia.

[13]  Jos T. A. Verhoeven,et al.  BIOMASS N:P RATIOS AS INDICATORS OF NUTRIENT LIMITATION FOR PLANT POPULATIONS IN WETLANDS , 2003 .

[14]  S. Güsewell,et al.  Variation in nitrogen and phosphorus concentrations of wetland plants , 2002 .

[15]  S. Woodin,et al.  Impacts of increased nitrogen supply on high Arctic heath: the importance of bryophytes and phosphorus availability. , 2001, The New phytologist.

[16]  J. Verhoeven,et al.  Decomposition of Carex and Sphagnum litter in two mesotrophic fens differing in dominant plant species , 2001 .

[17]  J. P. Grime,et al.  The effects of trophic structure and soil fertility on the assembly of plant communities: a microcosm experiment , 2000 .

[18]  Simulation of the effects of nutrient enrichment on nutrient and carbon dynamics in a river marginal wetland , 2000 .

[19]  Sarah E. Hobbie,et al.  Heterotrophic nitrogen fixation in decomposing litter: Patterns and regulation , 2000 .

[20]  H. Rydin,et al.  Nitrogen fertilization reduces Sphagnum production in bog communities. , 2000, The New phytologist.

[21]  P. Vitousek,et al.  NUTRIENT LIMITATION OF DECOMPOSITION IN HAWAIIAN FORESTS , 2000 .

[22]  R. Bobbink,et al.  Natural nitrogen filter fails in polluted raised bogs , 2000 .

[23]  K. Gross,et al.  Fertilization effects on species density and primary productivity in herbaceous plant communities , 2000 .

[24]  U. Bollens.  Effects of nutrient inputs and water regime on wetland vegetation and the performance of wetland species , 2000 .

[25]  Barbara L. Bedford,et al.  PATTERNS IN NUTRIENT AVAILABILITY AND PLANT DIVERSITY OF TEMPERATE NORTH AMERICAN WETLANDS , 1999 .

[26]  M. El-Kahloun,et al.  Phosphorus fertilization in a phosphorus‐limited fen: effects of timing , 1999 .

[27]  C. Körner,et al.  Interactive effects of elevated CO2, P availability and legume presence on calcareous grassland: results of a glasshouse experiment , 1999 .

[28]  A. Buttler,et al.  The fate of NH4NO3 added to Sphagnum magellanicum carpets at five European mire sites , 1999 .

[29]  A. Grootjans,et al.  Nutrient limitation and vegetation changes in a coastal dune slack , 1999 .

[30]  D. Silcock,et al.  Nitrogen-phosphorus interactions in peat , 1999 .

[31]  Jan G. M. Roelofs,et al.  The effects of air‐borne nitrogen pollutants on species diversity in natural and semi‐natural European vegetation , 1998 .

[32]  M. Wassen,et al.  NUTRIENT LIMITATION IN THE BIEBRZA FENS AND FLOODPLAIN (POLAND) , 1998 .

[33]  A. Grootjans,et al.  Nutrient deficiency in dune slack pioneer vegetation: a review , 1997 .

[34]  P. Ryser,et al.  Phosphorus allocation and utilization in three grass species with contrasting response to N and P supply. , 1997, The New phytologist.

[35]  D. Boeye,et al.  Nutrient limitation in species‐rich lowland fens , 1997 .

[36]  P. Vitousek,et al.  Nutrient limitation and soil development: Experimental test of a biogeochemical theory , 1997 .

[37]  M. Oorschot,et al.  EFFECTS OF ENHANCED NUTRIENT AVAILABILITY ON PLANT AND SOIL NUTRIENT DYNAMICS IN TWO ENGLISH RIVERINE ECOSYSTEMS , 1997 .

[38]  H. Kroon,et al.  Clonal plant dominance under elevated nitrogen deposition, with special reference to Brachypodium pinnatum in chalk grassland. , 1997 .

[39]  R. Marrs,et al.  Trends in soil chemistry and floristics associated with the establishment of a low-input meadow system on an arable clay soil in Essex, England. , 1997 .

[40]  R. Aerts,et al.  Nutritional and plant-mediated controls on leaf litter decomposition of Carex species , 1997 .

[41]  A. Kooijman,et al.  Vegetation succession in a floating mire in relation to management and hydrology , 1996 .

[42]  W. Koerselman,et al.  The vegetation N:P ratio: a new tool to detect the nature of nutrient limitation , 1996 .

[43]  J. Verhoeven,et al.  Nitrogen- or phosphorus-limited growth in herbaceous, wet vegetation: relations with atmospheric inputs and management regimes. , 1996, Trends in ecology & evolution.

[44]  B. Wheeler.  Restoration of temperate wetlands. , 1996 .

[45]  J. Willems,et al.  Long-term after effects of fertilization on above-ground phytomass and species diversity in calcareous grassland , 1996 .

[46]  Degradation and restoration of wet and moist meadows in the Czech Republic : general trends and case studies , 1996 .

[47]  D. Tilman,et al.  Convergence and divergence of old-field vegetation after 11 yr of nitrogen addition , 1995 .

[48]  F. Chapin,et al.  Long-term responses to factorial, NPK fertilizer treatment by Alaskan wet and moist tundra sedge species , 1995 .

[49]  F. Stuart Chapin,et al.  Responses of Arctic Tundra to Experimental and Observed Changes in Climate , 1995 .

[50]  H. Olff,et al.  Nutrient dynamics during restoration of fen meadows by hay making without fertilizer application. , 1995 .

[51]  J. Bakker,et al.  NUTRIENT LIMITATION AFTER LONG-TERM NITROGEN FERTILIZER APPLICATION IN CUT GRASSLANDS , 1994 .

[52]  W. Bowman.  Accumulation and use of nitrogen and phosphorus following fertilization in two alpine tundra communities , 1994 .

[53]  E. Hogg,et al.  Microsite and regional variation in the potential decay rate of Sphagnum magellanicum in south Swedish raised bogs , 1994 .

[54]  V. Nams,et al.  Effects of Nitrogen-Fertilization on several Woody and Nonwoody Boreal Forest Species , 1993 .

[55]  R. Aerts,et al.  Seasonal allocation of biomass and nitrogen in four Carex species from mesotrophic and eutrophic fens as affected by nitrogen supply , 1992 .

[56]  S. Jonasson.  Plant responses to fertilization and species removal in tundra related to community structure and clonality. , 1992 .

[57]  R. Aerts,et al.  Growth-Limiting Nutrients in Sphagnum-Dominated Bogs Subject to Low and High Atmospheric Nitrogen Supply , 1992 .

[58]  F. Berendse,et al.  Experiments on the restoration of species-rich meadows in The Netherlands , 1992 .

[59]  F. L. Twenhöven.  Competition between two Sphagnum species under different deposition levels , 1992 .

[60]  J. Verhoeven,et al.  Control of plant growth by nitrogen and phosphorus in mesotrophic fens , 1991 .

[61]  F. Chapin,et al.  Production: Biomass Relationships and Element Cycling in Contrasting Arctic Vegetation Types , 1991 .

[62]  Robert W. Howarth,et al.  Nitrogen limitation on land and in the sea: How can it occur? , 1991 .

[63]  J. Morris,et al.  Effects of Nitrogen Loading on Wetland Ecosystems with Particular Reference to Atmospheric Deposition , 1991 .

[64]  J. Roelofs,et al.  Impact of carbon dioxide and ammonium on the growth of submerged Sphagnum cuspidatum , 1991 .

[65]  J. Verhoeven,et al.  Nitrogen and phosphorus mineralization in fens and bogs. , 1990 .

[66]  W. Koerselman,et al.  Nitrogen, phosphorus and potassium budgets for two small fens surrounded by heavily fertilized pastures. , 1990 .

[67]  R. Marrs,et al.  A comparison of soil fertility between semi-natural and agricultural plant communities: Implications for the creations of species-rich grassland on abondoned agricultural land , 1990 .

[68]  W. Koerselman,et al.  Denitrification and dinitrogen fixation in two quaking fens in the Vechtplassen area, The Netherlands , 1989 .

[69]  David Tilman,et al.  Ecological Experimentation: Strengths and Conceptual Problems , 1989 .

[70]  P. Keddy,et al.  Conservation of wetlands: do infertile wetlands deserve a higher priority? , 1989 .

[71]  A. Kapfer.  Versuche zur Renaturierung gedüngten Feuchtgrünlandes , 1988 .

[72]  J. Verhoeven,et al.  Comparative emographic study of Carex rostrata stokes, C. diandra schrank and C. acutiformis EHRH. In fens of different nutrient status , 1988 .

[73]  J. Verhoeven,et al.  THE VEGETATION OF FENS IN RELATION TO THEIR HYDROLOGY AND NUTRIENT DYNAMICS: A CASE STUDY , 1988 .

[74]  H. Rudolph,et al.  Effects of NH+4‐N and NO+3‐N on growth and metabolism of Sphagnum magellanicum , 1986 .

[75]  Th. Egloff.  Auswirkungen und Beseitigung von Düngungseinflüssen auf Streuwiesen , 1986 .

[76]  J. Vermeer.  The effect of nutrients on shoot biomass and species composition of wetland and hayfield communities. , 1986 .

[77]  D. Tilman.  Resource competition and community structure. , 1983, Monographs in population biology.

[78]  J. Lee,et al.  NITRATE REDUCTASE ACTIVITY OF SPHAGNUM SPECIES IN THE SOUTH PENNINES , 1982 .

[79]  R. Leonard,et al.  ROOT EXUDATION IN RELATION TO SUPPLY OF PHOSPHORUS AND ITS POSSIBLE RELEVANCE TO MYCORRHIZAL FORMATION , 1978 .

[80]  U. Granhall,et al.  Nitrogen fixation in a subarctic mire , 1973 .

[81]  S. Haslam,et al.  The development of shoots in Phragmites communis Trin. , 1969 .