DNA-modified electrodes. Part 2. Electrochemical characterization of gold electrodes modified with DNA

Abstract Gold electrodes were modified with DNA by adsorption. The DNA-modified electrodes were electrochemically characterized with Co(bpy) 3 3+ , a electroactive DNA-binding complex, as an indicator. It is interesting that the pair of redox peaks of Co(bpy) 3 3+ split into two pairs at dsDNA-modified gold electrodes. One pair of peaks shifts negative, and the peak current increases notably; another pair of peaks shifts positive. These suggest that dsDNA has been immobilized onto gold electrode surfaces and the layer of dsDNA on the surfaces can bind Co(bpy) 3 3+ in two different ways. Gold electrodes can be modified also with ssDNA by adsorption but only one pair of peaks of Co(bpy) 3 3+ appears at ssDNA-modified gold electrodes. The amount of Co(bpy) 3 3+ enriched by the layers of dsDNA or ssDNA adsorbed at gold electrodes was estimated from the peak charge of Co(bpy) 3 3+ reduction at the electrodes obtained by CV. The stability of the DNA-modified electrodes was investigated. The DNA modification layer on gold surfaces is unstable to alkali and to heat, but stable to acid solutions and very stable in long stock in a dry state. A comparison of modifications of gold, platinum and glassy carbon with DNA was carried out.

[1]  A. Bard,et al.  Electrogenerated Chemiluminescence. 55. Emission from Adsorbed Ru(bpy)32+ on Graphite, Platinum, and Gold , 1994 .

[2]  V. Brabec,et al.  Electrochemical analysis of antitumour platinum drugs and their complexes with DNA , 1988 .

[3]  M. Maeda,et al.  DNA-Immobilized Gold Electrode for DNA-Binding Drug Sensor , 1992 .

[4]  Yoshio Ishimori,et al.  Novel DNA sensor for electrochemical gene detection , 1994 .

[5]  A. Bard,et al.  Electrochemical studies of the interaction of metal chelates with DNA. 4. Voltammetric and electrogenerated chemiluminescent studies of the interaction of tris(2,2'-bipyridine)osmium(II) with DNA. , 1990, Analytical chemistry.

[6]  E. Paleček,et al.  Electrochemical behaviour of biological macromolecules , 1986 .

[7]  Peter E. Nielsen,et al.  Peptide Nucleic Acid Probes for Sequence-Specific DNA Biosensors , 1996 .

[8]  Michael T. Carter,et al.  Voltammetric studies of the interaction of metal chelates with DNA. 2. Tris-chelated complexes of cobalt(III) and iron(II) with 1,10-phenanthroline and 2,2'-bipyridine , 1989 .

[9]  A. Bard,et al.  Immobilization and Hybridization of DNA on an Aluminum(III) Alkanebisphosphonate Thin Film with Electrogenerated Chemiluminescent Detection , 1995 .

[10]  Susan R. Mikkelsen,et al.  Covalent immobilization of DNA onto glassy carbon electrodes , 1992 .

[11]  N. Turro,et al.  Accelerated electron transfer between metal complexes mediated by DNA. , 1988, Science.

[12]  R. Parsons,et al.  Comparison between the behaviour of native and denatured DNA at mercury and gold electrodes by capacity measurements and cyclic voltammetry , 1981 .

[13]  T. Mallouk,et al.  Immobilization of DNA on an Aluminum(III) Alkanebisphosphonate Thin Film with Electrogenerated Chemiluminescent Detection , 1994 .

[14]  A. Bard,et al.  Voltammetric studies of the interaction of tris(1,10-phenanthroline)cobalt(III) with DNA , 1987 .

[15]  M. Vojtíṥková,et al.  Determination of Submicrogram Quantities of Circular Duplex DNA in Plasmid Samples By Adsorptive Stripping Voltammetry , 1987 .

[16]  D. Pang,et al.  Modification of glassy carbon and gold electrodes with DNA , 1996 .

[17]  I. Miller.  The structure of DNA and RNA in the water-mercury interface. , 1961, Journal of molecular biology.

[18]  D. Pang,et al.  Electrochemical oxidation of DNA at a gold microelectrode , 1995 .

[19]  K. Hashimoto,et al.  Sequence-specific gene detection with a gold electrode modified with DNA probes and an electrochemically active dye. , 1994, Analytical chemistry.

[20]  E. Paleček,et al.  From polarography of DNA to microanalysis with nucleic acid-modified electrodes , 1996 .