Identification and characterization of a conserved outer‐membrane protein of Neisseria gonorrhoeae

A previous study in our laboratory identified a surface‐exposed peptidoglycan‐associated protein of Neisseria gonorrhoeae which had an apparent molecular mass of 44000 daltons (44kDa) (Hill and Judd, 1989). This paper reports results which confirm that the 44 kDa protein is surface‐exposed, and that the protein is expressed in, and is structurally invariant among, 14 strains of N. gonorrhoeae. The fact that the 44kDa outer‐membrane protein is found in a conserved form in all gonococci examined strongly suggests that ft is crucial to the bacterium's survival. Moreover, it appears that this protein is a penicillin‐binding protein (PBP3) (Shafer and Judd, 1991). This invariant, surface‐exposed, peptidoglycan‐associated outer‐membrane protein deserves further investigation to elucidate its role in the immunobiology of N. gonorrhoeae, and its possible use as an immunoprophylactic reagent.

[1]  W. Shafer,et al.  Gonococcal penicillin‐binding protein 3 and the surface‐exposed 44kDa peptidoglycan‐binding protein appear to be the same molecule , 1991, Molecular microbiology.

[2]  J. Cannon,et al.  Characterization of the repertoire of hypervariable regions in the Protein II (opa) gene family of Neisseria gonorrhoeae , 1990, Molecular microbiology.

[3]  S. Hill,et al.  Identification and characterization of peptidoglycan-associated proteins in Neisseria gonorrhoeae , 1989, Infection and immunity.

[4]  M. Virji,et al.  The potential protective effect of immunization with outer-membrane protein I from Neisseria gonorrhoeae. , 1989, Journal of general microbiology.

[5]  M. Virji,et al.  Location of a blocking epitope on outer-membrane protein III of Neisseria gonorrhoeae by synthetic peptide analysis. , 1989, Journal of general microbiology.

[6]  W. Shafer,et al.  Topographical alterations in proteins I of Neisseria gonorrhoeae correlated with lipooligosaccharide variation , 1989, Molecular microbiology.

[7]  D. Dorward,et al.  Export and intercellular transfer of DNA via membrane blebs of Neisseria gonorrhoeae , 1989, Journal of bacteriology.

[8]  R. C. Judd.  Protein I: structure, function, and genetics , 1989, Clinical Microbiology Reviews.

[9]  J. Heckels.  Structure and function of pili of pathogenic Neisseria species , 1989, Clinical Microbiology Reviews.

[10]  R. C. Judd.  Purification of outer membrane proteins of the gram-negative bacterium Neisseria gonorrhoeae. , 1988, Analytical biochemistry.

[11]  G. Murphy,et al.  Recombination among Protein II genes of Neisseria gonorrhoeae generates new coding sequences and increases structural variability in the Protein II family , 1988, Molecular microbiology.

[12]  M. Apicella,et al.  Selection and immunochemical analysis of lipooligosaccharide mutants of Neisseria gonorrhoeae , 1988, Infection and immunity.

[13]  N. Carbonetti,et al.  Molecular cloning and characterization of the structural gene for protein I, the major outer membrane protein of Neisseria gonorrhoeae. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[14]  M. Koomey,et al.  Porin protein of Neisseria gonorrhoeae: cloning and gene structure. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[15]  J. Griffiss,et al.  Phenotypic variation in epitope expression of the Neisseria gonorrhoeae lipooligosaccharide , 1987, Infection and immunity.

[16]  T. Evans,et al.  Induction and repression of outer membrane proteins by anaerobic growth of Neisseria gonorrhoeae , 1987, Infection and immunity.

[17]  G. Schoolnik,et al.  Purification and characterization of the major iron-regulated protein expressed by pathogenic Neisseriae , 1987, The Journal of experimental medicine.

[18]  R. C. Judd.  Radioiodination and 125I-labeled peptide mapping of proteins on nitrocellulose membranes. , 1987, Analytical biochemistry.

[19]  R. C. Judd,et al.  Characterization of protein I from serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae , 1987 .

[20]  P. Rice,et al.  Immunoglobulin G antibodies directed against protein III block killing of serum-resistant Neisseria gonorrhoeae by immune serum , 1986, The Journal of experimental medicine.

[21]  K. Joiner,et al.  Monoclonal antibodies directed against gonococcal protein I vary in bactericidal activity. , 1985, Journal of immunology.

[22]  R. C. Judd.  Structure and surface exposure of protein IIs of Neisseria gonorrhoeae JS3 , 1985, Infection and immunity.

[23]  I. Nachamkin,et al.  Monoclonal antibody that recognizes an outer membrane antigen common to the pathogenic Neisseria species but not to most nonpathogenic Neisseria species , 1984, Infection and immunity.

[24]  K. Zak,et al.  Antigenic variation during infection with Neisseria gonorrhoeae: detection of antibodies to surface proteins in sera of patients with gonorrhea. , 1984, The Journal of infectious diseases.

[25]  D. Smith,et al.  Lactoperoxidase and Iodo-Gen-catalyzed iodination labels inner and outer membrane proteins of Haemophilus influenzae , 1983, Journal of bacteriology.

[26]  K. Chen,et al.  Serology of Neisseria gonorrhoeae: coagglutination serogroups WI and WII/III correspond to different outer membrane protein I molecules , 1982, Infection and immunity.

[27]  R. C. Judd.  125I-peptide mapping of protein III isolated from four strains of Neisseria gonorrhoeae , 1982, Infection and immunity.

[28]  R. C. Judd.  Surface peptide mapping of protein I and protein III of four strains of Neisseria gonorrhoeae , 1982, Infection and immunity.

[29]  J. Swanson.  Colony opacity and protein II compositions of gonococci , 1982, Infection and immunity.

[30]  J. Swanson.  Studies on gonococcus infection. XVIII. 125I-labeled peptide mapping of the major protein of the gonococcal cell wall outer membrane , 1979, Infection and immunity.

[31]  Fox Cf,et al.  Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. , 1978 .

[32]  K. Karlsson,et al.  Identification and characterization of a Neisseria gonorrhoeae gene encoding a glycolipid-binding adhesin. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[33]  K. Joiner,et al.  Characterization of protein I from serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae. , 1987, Infection and immunity.