Ochrobactrum anthropi, contaminants of in vitro culture of sugarcane cells and tissues

El proposito de este trabajo fue informar, por primera vez, la presencia de Ochrobactrum anthropi como contaminante del cultivo in vitro de celulas y tejidos de cana de azucar. La bacteria contaminante de las plantas in vitro se aislo directamente en el medio de cultivo Agar Triptona soya a partir de plantas visiblemente contaminadas y algunas gotas de suspensiones celulares contaminadas se inocularon en tubos de ensayo con 3ml de Caldo Triptona soya. Las bacterias aisladas se examinaron microscopicamente (forma, motilidad) y mediante prueba bioquimicas (tincion de Gram; modificacion de Hucker), oxidasa, catalasa, O/F (oxidacion/fermentacion). Otras pruebas adicionales fueron realizadas de acuerdo con lo descrito en el Manual de Bergey. La identificacion se complemento por analisis de acidos grasos. Diferentes bacterias pueden aparecer contaminando el cultivo in vitro de celulas y tejidos vegetales, sin embargo, no se han encontrado referencias sobre la presencia de Ochrobactrum anthropi como contaminante del cultivo in vitro de cana de azucar. Para controlar los contaminantes bacterianos es necesario primero identificarlos

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