Genotoxicity and ecotoxicity assays using the freshwater crustacean Daphnia magna and the larva of the aquatic midge Chironomus riparius to screen the ecological risks of nanoparticle exposure.

Genotoxic and ecotoxic assessments of widely used nanoparticles, cerium dioxide (CeO(2)), silicon dioxide (SiO(2)) and titanium dioxide (TiO(2)), were conducted on two aquatic sentinel species, the freshwater crustacean Daphnia magna and the larva of the aquatic midge Chironomus riparius. CeO(2) may have genotoxic effects on D. magna and C. riparius, given that the DNA strand breaks increased in both species when exposed to this nanoparticle; whereas, neither exposure to SiO(2) nor TiO(2) had a genotoxic effect on either species. A statistically significant correlation was observed between DNA damage and mortality in the CeO(2)-exposed C. riparius, which suggests that CeO(2)-induced DNA damage might provoke higher-level consequences. SiO(2) did not seem to affect the DNA integrity; whereas, the mortality of both the SiO(2)-exposed D. magna and C. riparius increased. The TiO(2) nanoparticle did not lead to significant alterations in geno- or ecotoxic parameters of both species. Overall, these results suggest that CeO(2) nanoparticles may be genotoxic toward aquatic organisms, which may contribute to the knowledge relating to the aquatic toxicity of the most widely used nanomaterials on aquatic ecosystems, for which little data are available.

[1]  M. Antonietti,et al.  Modification of TiO2 Network Structures Using a Polymer Gel Coating Technique , 2001 .

[2]  Rebecca Klaper,et al.  Behavioral and physiological changes in Daphnia magna when exposed to nanoparticle suspensions (titanium dioxide, nano- , 2008 .

[3]  Shurong Wang,et al.  Preparation and characterization of CuO/CeO2 catalysts and their applications in low-temperature CO oxidation , 2005 .

[4]  H. Segner,et al.  Comet assay with the fish cell line rainbow trout gonad‐2 for in vitro genotoxicity testing of xenobiotics and surface waters , 2005, Environmental toxicology and chemistry.

[5]  Jinhee Choi,et al.  Oxidative stress of CeO2 nanoparticles via p38-Nrf-2 signaling pathway in human bronchial epithelial cell, Beas-2B. , 2009, Toxicology letters.

[6]  J. Schlager,et al.  In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. , 2005, Toxicological sciences : an official journal of the Society of Toxicology.

[7]  Jinhee Choi,et al.  Oxidative stress induced by cerium oxide nanoparticles in cultured BEAS-2B cells. , 2008, Toxicology.

[8]  Rebecca Klaper,et al.  Behavioral and physiological changes in Daphnia magna when exposed to nanoparticle suspensions (titanium dioxide, nano-C60, and C60HxC70Hx). , 2007, Environmental science & technology.

[9]  P. Gramatica.  In Memory of Professor Davide Calamari , 2006 .

[10]  J. Giesy,et al.  Comparison of three sediment bioassay methods using detroit river sediments , 1988 .

[11]  Avelino Corma,et al.  Synthesis and catalytic properties of thermally and hydrothermally stable, high-surface-area SiO2–CeO2 mesostructured composite materials and their application for the removal of sulfur compounds from gasoline , 2004 .

[12]  Dan Luo,et al.  Cellular Fate of a Modular DNA Delivery System Mediated by Silica Nanoparticles , 2008, Biotechnology progress.

[13]  J. Koberstein,et al.  Copper oxide nanocrystals. , 2005, Journal of the American Chemical Society.

[14]  Dong Suk Kim,et al.  Photocatalytic inactivation of E. coli with a mesoporous TiO2 coated film using the film adhesion method. , 2009, Environmental science & technology.

[15]  M D Waters,et al.  Genetic toxicology and risk assessment of complex environmental mixtures. , 1996, Drug and chemical toxicology.

[16]  M. Kikuchi,et al.  Screening of organophosphate insecticide pollution in water by using Daphnia magna. , 2000, Ecotoxicology and environmental safety.

[17]  M. Depledge,et al.  Fitness parameters and DNA effects are sensitive indicators of copper-induced toxicity in Daphnia magna. , 2001, Toxicological sciences : an official journal of the Society of Toxicology.

[18]  Andrew Murray,et al.  Cell cycle: A snip separates sisters , 1999, Nature.

[19]  M. Grätzel.  Mesoporous oxide junctions and nanostructured solar cells , 1999 .

[20]  Ichiro Matsubara,et al.  Development of Resistive Oxygen Sensors Based on Cerium Oxide Thick Film , 2004 .

[21]  G. Oberdörster,et al.  Nanotoxicology: An Emerging Discipline Evolving from Studies of Ultrafine Particles , 2005, Environmental health perspectives.

[22]  Qiao Wan,et al.  Simultaneous assay of glucose, lactate, L-glutamate and hypoxanthine levels in a rat striatum using enzyme electrodes based on neutral red-doped silica nanoparticles , 2004, Analytical and bioanalytical chemistry.

[23]  S. A. Barnett,et al.  A direct-methane fuel cell with a ceria-based anode , 1999, Nature.

[24]  J. Gearhart,et al.  In vitro toxicity of nanoparticles in BRL 3A rat liver cells. , 2005, Toxicology in vitro : an international journal published in association with BIBRA.

[25]  M. Anpo,et al.  The effect of ultraviolet functionalization of titanium on integration with bone. , 2009, Biomaterials.

[26]  J. James,et al.  Pulmonary toxicity of single-wall carbon nanotubes in mice 7 and 90 days after intratracheal instillation. , 2003, Toxicological sciences : an official journal of the Society of Toxicology.

[27]  Jongki Hong,et al.  Ecotoxicological evaluation of octachlorostyrene in fourth instar larvae of Chironomus riparius (Diptera, chironomidae) , 2008, Environmental toxicology and chemistry.

[28]  Avelino Corma,et al.  Hierarchically mesostructured doped CeO2 with potential for solar-cell use , 2004, Nature materials.

[29]  R. Nemanich,et al.  Multi-walled carbon nanotube interactions with human epidermal keratinocytes. , 2005, Toxicology letters.

[30]  P. Kamat,et al.  TiO2-graphene nanocomposites. UV-assisted photocatalytic reduction of graphene oxide. , 2008, ACS nano.

[31]  Kerstin Hund-Rinke,et al.  Ecotoxic Effect of Photocatalytic Active Nanoparticles (TiO2) on Algae and Daphnids (8 pp) , 2006, Environmental science and pollution research international.

[32]  R. Tice,et al.  A simple technique for quantitation of low levels of DNA damage in individual cells. , 1988, Experimental cell research.

[33]  Jinhee Choi,et al.  Multilevel evaluation of nonylphenol toxicity in fourth‐instar larvae of Chironomus riparius (Diptera, Chironomidae) , 2006, Environmental toxicology and chemistry.

[34]  Akira Fujishima,et al.  Titanium dioxide photocatalysis , 2000 .

[35]  J. West,et al.  Correlating nanoscale titania structure with toxicity: a cytotoxicity and inflammatory response study with human dermal fibroblasts and human lung epithelial cells. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.

[36]  J. Férard,et al.  Comet assay in genetic ecotoxicology: A review , 1999, Environmental and molecular mutagenesis.

[37]  Rebecca Klaper,et al.  Daphnia magna mortality when exposed to titanium dioxide and fullerene (C60) nanoparticles , 2006, Environmental toxicology and chemistry.

[38]  P. Armitage,et al.  The Chironomidae: the biology and ecology of non-biting midges. , 1995 .

[39]  N. Shibata,et al.  Liquid filled nanoparticles as a drug delivery tool for protein therapeutics. , 2005, Biomaterials.

[40]  Sun Young Park,et al.  Cytotoxicity, genotoxicity and ecotoxicity assay using human cell and environmental species for the screening of the risk from pollutant exposure. , 2007, Environment international.

[41]  K. Wakabayashi,et al.  Mutagens in surface waters: a review. , 2004, Mutation research.

[42]  R. Stafford,et al.  Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[43]  Robert N Grass,et al.  Exposure of engineered nanoparticles to human lung epithelial cells: influence of chemical composition and catalytic activity on oxidative stress. , 2007, Environmental science & technology.

[44]  Mamiko Sato,et al.  Size-dependent toxicity of silica nano-particles to Chlorella kessleri , 2008, Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering.

[45]  H. Roche,et al.  Effects of physical (hypoxia, hyperoxia) and chemical (potassium dichromate, fenitrothion) stress on antioxidant enzyme activities in Chironomus riparius mg. (diptera, chironomidae) larvae: Potential biomarkers , 2000 .

[46]  Ecotoxicity of nanomaterials to fish: Challenges for ecotoxicity testing , 2007, Integrated environmental assessment and management.