DNA barcoding of Iberian Peninsula and North Africa Tawny Owls Strix aluco suggests the Strait of Gibraltar as an important barrier for phylogeography

Abstract Eight subspecies have been proposed within the Tawny Owl (Strix aluco) species. However, recent molecular data have challenged this view, encouraging further work in this species complex. Here we reevaluated the taxonomic status between the North-Western African Tawny Owl, S. a. mauritanica, and its closest Iberian Tawny Owl population (from the S. a. sylvatica to S. a. aluco clade) separated by the Strait of Gibraltar. The Tawny Owl is a non-migratory and territorial species, and juvenile dispersal is restricted to a few kilometers around the natal site. This limited dispersal and the barrier imposed by the Strait of Gibraltar predicted a strong differentiation between the two populations. We tested this using DNA barcoding, Bayesian phylogenetic and species delimitation analysis. We found that an 81.1% of variation is due to the intergroups variation. In addition, the inter–intraspecific distances distribution revealed a barcoding gap among the two subspecies. Also, posterior probabilities and the PAB value allowed to reject the hypothesis that observed degree of distinctiveness is due to random coalescence processes. These findings clearly support the Strait of Gibraltar as an isolating barrier for this species. The subspecific status is confirmed and species status is even suggested for S. a. mauritanica.

[1]  W. Ulrich,et al.  Palaearctic biogeography revisited: evidence for the existence of a North African refugium for Western Palaearctic biota , 2014 .

[2]  John-James Wilson,et al.  Building a DNA Barcode Reference Library for the True Butterflies (Lepidoptera) of Peninsula Malaysia: What about the Subspecies? , 2013, PloS one.

[3]  Sujeevan Ratnasingham,et al.  A DNA-Based Registry for All Animal Species: The Barcode Index Number (BIN) System , 2013, PloS one.

[4]  T. Backeljau,et al.  Exploring Species Level Taxonomy and Species Delimitation Methods in the Facultatively Self-Fertilizing Land Snail Genus Rumina (Gastropoda: Pulmonata) , 2013, PloS one.

[5]  K. Katoh,et al.  MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability , 2013, Molecular biology and evolution.

[6]  H. Higuchi,et al.  Does the Short-tailed Albatross Phoebastria albatrus consist of two species!? , 2012 .

[7]  S. Boyer,et al.  Spider: An R package for the analysis of species identity and evolution, with particular reference to DNA barcoding , 2012, Molecular ecology resources.

[8]  A. Lambert,et al.  ABGD, Automatic Barcode Gap Discovery for primary species delimitation , 2012, Molecular ecology.

[9]  Maxim Teslenko,et al.  MrBayes 3.2: Efficient Bayesian Phylogenetic Inference and Model Choice Across a Large Model Space , 2012, Systematic biology.

[10]  H. Higuchi,et al.  Inferring the ancient population structure of the vulnerable albatross Phoebastria albatrus, combining ancient DNA, stable isotope, and morphometric analyses of archaeological samples , 2012, Conservation Genetics.

[11]  K. Shah,et al.  In search of function for hypothetical proteins encoded by genes of SA-JA pathways in Oryza sativa by in silico comparison and structural modeling , 2012, Bioinformation.

[12]  L. Kubatko,et al.  Species Delimitation and Global Biosecurity , 2011, Evolutionary bioinformatics online.

[13]  Vicky Fan,et al.  Species delimitation – a geneious plugin for the exploration of species boundaries , 2011, Molecular ecology resources.

[14]  Aurélien Miralles,et al.  The integrative future of taxonomy , 2010, Frontiers in Zoology.

[15]  L. Excoffier,et al.  Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows , 2010, Molecular ecology resources.

[16]  Pablo Librado,et al.  DnaSP v5: a software for comprehensive analysis of DNA polymorphism data , 2009, Bioinform..

[17]  P. Hebert,et al.  Comprehensive DNA barcode coverage of North American birds , 2007, Molecular ecology notes.

[18]  P. Hebert,et al.  DNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics. , 2007, Trends in genetics : TIG.

[19]  N. Rosenberg.  STATISTICAL TESTS FOR TAXONOMIC DISTINCTIVENESS FROM OBSERVATIONS OF MONOPHYLY , 2007, Evolution; international journal of organic evolution.

[20]  Gaurav Vaidya,et al.  DNA barcoding and taxonomy in Diptera: a tale of high intraspecific variability and low identification success. , 2006, Systematic biology.

[21]  Alfried P Vogler,et al.  Sequence-based species delimitation for the DNA taxonomy of undescribed insects. , 2006, Systematic biology.

[22]  Paul D N Hebert,et al.  DNA barcodes reveal cryptic host-specificity within the presumed polyphagous members of a genus of parasitoid flies (Diptera: Tachinidae). , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[23]  Q. Wheeler,et al.  The perils of DNA barcoding and the need for integrative taxonomy. , 2005, Systematic biology.

[24]  Patrícia H. Brito,et al.  The influence of Pleistocene glacial refugia on tawny owl genetic diversity and phylogeography in western Europe , 2005, Molecular ecology.

[25]  B. Dayrat,et al.  Towards integrative taxonomy , 2005 .

[26]  P. Hebert,et al.  Identification of Birds through DNA Barcodes , 2004, PLoS biology.

[27]  J. Croxall,et al.  Global population structure and taxonomy of the wandering albatross species complex , 2004, Molecular ecology.

[28]  M. Ferdig,et al.  DNA barcoding of parasites and invertebrate disease vectors: what you don't know can hurt you. , 2003, Trends in parasitology.

[29]  C. Abbott,et al.  Phylogeography of shy and white‐capped albatrosses inferred from mitochondrial DNA sequences: implications for population history and taxonomy , 2003, Molecular ecology.

[30]  F. Henrique-Silva,et al.  Large scale sex typing of ostriches using DNA extracted from feathers , 2002, BMC biotechnology.

[31]  J. Croxall,et al.  Global relationships amongst black‐browed and grey‐headed albatrosses: analysis of population structure using mitochondrial DNA and microsatellites , 2001, Molecular ecology.

[32]  R. J. Gutiérrez,et al.  PHYLOGEOGRAPHY OF SPOTTED OWL (STRIX OCCIDENTALIS) POPULATIONS BASED ON MITOCHONDRIAL DNA SEQUENCES: GENE FLOW, GENETIC STRUCTURE, AND A NOVEL BIOGEOGRAPHIC PATTERN , 1999, Evolution; international journal of organic evolution.

[33]  P. Galeotti,et al.  Rufous and Grey Colour Morphs in the Italian Tawny Owl: Geographical and Environmental Influences , 1996 .

[34]  C. Perrins,et al.  Birds of the Western Palearctic , 1978, Nature.

[35]  H. Weimerskirch,et al.  Piecing together the global population puzzle of wandering albatrosses: genetic analysis of the Amsterdam albatross Diomedea amsterdamensis , 2011 .

[36]  C. F. Coles,et al.  Dispersal behavior and survival of juvenile Tawny Owls (Strix aluco) during the low point in a vole cycle , 1997 .

[37]  L. Svensson,et al.  The Birds of the Western Palearctic , 1977 .