Theoretical principles of in vitro selection using combinatorial nucleic acid libraries.

A new paradigm for drug discovery and biological research has developed from technologies that integrate combinatorial chemistry with rounds of selection and amplification, a technique called in vitro selection or systematic evolution of ligands by exponential enrichment (SELEX). This overview unit discusses nucleic acid libraries that can be used, affinity probability distributions, an equilibrium model for SELEX, and optimal conditions including concentrations and signal‐to‐noise ratios.

[1]  L. Gold,et al.  The mathematics of SELEX against complex targets. , 1998, Journal of molecular biology.

[2]  B Levitan,et al.  Stochastic modeling and optimization of phage display. , 1998, Journal of molecular biology.

[3]  D. Bartel,et al.  Accessing rare activities from random RNA sequences: the importance of the length of molecules in the starting pool. , 1997, Chemistry & biology.

[4]  Ronald R. Breaker,et al.  In Vitro Selection of Catalytic Polynucleotides. , 1997, Chemical reviews.

[5]  J W Szostak,et al.  Ribozymes: aiming at RNA replication and protein synthesis. , 1996, Chemistry & biology.

[6]  M. McClelland,et al.  DNA rehybridization during PCR: the 'Cot effect' and its consequences. , 1996, Nucleic acids research.

[7]  David J. Galas,et al.  A Mathematical Analysis ofin VitroMolecular Selection – Amplification , 1996 .

[8]  I. Majerfeld,et al.  Affinity selection-amplification from randomized ribooligonucleotide pools. , 1996, Methods in enzymology.

[9]  M S Waterman,et al.  A mathematical analysis of in vitro molecular selection-amplification. , 1996, Journal of molecular biology.

[10]  P. Schuster,et al.  How to search for RNA structures. Theoretical concepts in evolutionary biotechnology. , 1995, Journal of biotechnology.

[11]  L. Gold,et al.  Oligonucleotides as Research, Diagnostic, and Therapeutic Agents(*) , 1995, The Journal of Biological Chemistry.

[12]  S. Kauffman,et al.  Search strategies for applied molecular evolution. , 1995, Journal of theoretical biology.

[13]  B. Kay,et al.  Biologically displayed random peptides as reagents in mapping protein-protein interactions , 1995 .

[14]  M Yarus,et al.  Diversity of oligonucleotide functions. , 1995, Annual review of biochemistry.

[15]  G. Winter,et al.  Making antibodies by phage display technology. , 1994, Annual review of immunology.

[16]  L. Gold,et al.  Selective enrichment of RNA species for tight binding to Escherichia coli rho factor , 1993, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[17]  C. Tuerk,et al.  SELEXION. Systematic evolution of ligands by exponential enrichment with integrated optimization by non-linear analysis. , 1991, Journal of molecular biology.

[18]  G. Stormo,et al.  Specificity of the Mnt protein determined by binding to randomized operators. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[19]  H. Weintraub,et al.  Differences and similarities in DNA-binding preferences of MyoD and E2A protein complexes revealed by binding site selection. , 1990, Science.

[20]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[21]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[22]  R. Barrett,et al.  Peptides on phage: a vast library of peptides for identifying ligands. , 1990, Proceedings of the National Academy of Sciences of the United States of America.

[23]  J. Scott,et al.  Searching for peptide ligands with an epitope library. , 1990, Science.

[24]  P. V. von Hippel,et al.  Selection of DNA binding sites by regulatory proteins. Statistical-mechanical theory and application to operators and promoters. , 1987, Journal of molecular biology.