EFFECTS OF SONICATION AND OTHER AGENTS ON THE ACTIONS OF MUSCLE MICROSOMES.

The effects of sonication and other agents on the calcium-binding and the relaxing activity of microsomes were studied and the following results were obtained.1. Sonication of microsomes destroyed the membraneous structure and enhanced the calcium-binding activity, whereas scarcely affected the relaxing activity of them.The heat treatment and ultraviolet irradiation produced the inhibitory effects on the calcium-binding and the relaxing activity.2. In the presence of oxalate, at the concentration of 0.001mM calcium the microsomes was able to bind calcium and the calculated value of the capacity of the microsomes for binding calcium was about 2.5 μM/mg protein.3. The addition of caffeine or nicotine scarcely inhibited the calciumbinding activity of the microsomes, whereas the addition of salyrgan, deoxycholate or ethylenediaminetetraacetate markedly did. By the addition of 2, 4, -dinitrophenol, furthermore, the calcium-binding rate was decreased.4. Inosinetriphosphate, uridinetriphosphate or adenosinediphosphate could be replaced with adenosinetriphosphate for the calcium-binding of the microsomes. Furthermore, KF did not inhibit the calcium-binding activity in the presence of adenosinetriphosphate, whereas markedly did in the presence of adenosinediphosphate.

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