Role of collagen and fibronectin in neural crest cell adhesion and migration.

Abstract Cultured neural crest cells which are freshly trypsinized require serum or purified fibronectin to attach to collagen substrates of types I–V. Furthermore, neural crest cells migrate in a Boyden chamber in response to fibronectin, and a “checkerboard” analysis demonstrates that fibronectin is both chemotactic and chemokinetic for these cells. It is proposed that collagen serves as a substrate for neural crest cells as they migrate in the early embryo. By mediating the cells' attachment to collagen, fibronectin may influence the movement of the cells. Local differences in fibronectin concentration or availability in the matrix could affect the degree of attachment of the cells to the collagen substrate and could also direct their migration by serving as a chemoattractant.

[1]  H. Kleinman,et al.  Role of attachment factors and attractants in fibroblast chemotaxis. , 1980, The Journal of laboratory and clinical medicine.

[2]  M. Derby.  Analysis of glycosaminoglycans within the extracellular environments encountered by migrating neural crest cells. , 1978, Developmental biology.

[3]  H. Furthmayr.  Immunochemistry of the extracellular matrix , 1982 .

[4]  N. L. Le Douarin,et al.  Restrictions of developmental capabilities in neural crest cell derivatives as tested by in vivo transplantation experiments. , 1980, Developmental biology.

[5]  J. Löfberg,et al.  Neural crest cell migration in relation to extracellular matrix organization in the embryonic axolotl trunk. , 1980, Developmental biology.

[6]  G. Martin,et al.  A murine tumor producing a matrix of basement membrane , 1977, The Journal of experimental medicine.

[7]  M. Bronner‐Fraser,et al.  Analysis of the neural crest ventral pathway using injected tracer cells. , 1980, Developmental biology.

[8]  M. C. Johnston.  A radioautographic study of the migration and fate of cranial neural crest cells in the chick embryo , 1966, The Anatomical record.

[9]  E. Epstein.  [α1(III)]3 Human Skin Collagen RELEASE BY PEPSIN DIGESTION AND PREPONDERANCE IN FETAL LIFE , 1974 .

[10]  R. Hynes,et al.  Effects of LETS glycoprotein on cell motility , 1978, Cell.

[11]  S. Gay,et al.  Recongnition by guinea-pig peritoneal exudate cells of conformationally different states of the collagen molecule. , 1976, Immunology.

[12]  V. Twitty,et al.  Causal analysis of chromatophore migration. , 1948, The Journal of experimental zoology.

[13]  R. Hynes,et al.  Immunocytochemical localization of fibronectin in embryonic chick trunk and area vasculosa. , 1981, Developmental biology.

[14]  H. Kleinman,et al.  Identification of an adhesion factor for chondrocytes. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[15]  J. Foidart,et al.  Laminin--a glycoprotein from basement membranes. , 1979, The Journal of biological chemistry.

[16]  B. Schrier,et al.  Development of choline acetyltransferase activity in chick cranial neural crest cells in culture. , 1977, Developmental biology.

[17]  I. Kaitila,et al.  Fetal membrane collagens: identification of two new collagen alpha chains. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[18]  K. Yamada,et al.  Cellular fibronectin promotes adrenergic differentiation of quail neural crest cells in vitro. , 1981, Experimental cell research.

[19]  C. Erickson,et al.  Analysis of migratory behavior of neural crest and fibroblastic cells in embryonic tissues. , 1980, Developmental biology.

[20]  Kenneth M. Yamada,et al.  Fibronectins—adhesive glycoproteins of cell surface and blood , 1978, Nature.

[21]  H. Kleinman,et al.  Connective tissue structure: cell binding to collagen. , 1978, The Journal of investigative dermatology.

[22]  G. Martin,et al.  Role of laminin in the attachment of PAM 212 (epithelial) cells to basement membrane collagen , 1980, Cell.

[23]  John Tyler Bonner,et al.  The Cellular Slime Molds. , 1967 .

[24]  H. Hörmann,et al.  Fibronectin (cold-insoluble globulin), VI. Influence of heparin and hyaluronic acid on the binding of native collagen. , 1979, Hoppe-Seyler's Zeitschrift fur physiologische Chemie.

[25]  W. Falk,et al.  A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. , 1980, Journal of immunological methods.

[26]  B. Toole.  Morphogenetic Role of Glycosaminoglycans (Acid Mucopolysaccharides) in Brain and Other Tissues , 1976 .

[27]  M A Teillet,et al.  Experimental analysis of the migration and differentiation of neuroblasts of the autonomic nervous system and of neurectodermal mesenchymal derivatives, using a biological cell marking technique. , 1974, Developmental biology.

[28]  S. Carter,et al.  Principles of Cell Motility: The Direction of Cell Movement and Cancer Invasion , 1965, Nature.

[29]  D. Noden.  An analysis of migratory behavior of avian cephalic neural crest cells. , 1975, Developmental biology.

[30]  J. Foidart,et al.  Collagen synthesis in cultures of differentiating neural crest cells. , 1980, Cell differentiation.

[31]  E. Ruoslahti,et al.  Distribution of fibroblast surface antigen in the developing chick embryo , 1975, The Journal of experimental medicine.

[32]  E. Pearlstein.  Plasma membrane glycoprotein which mediates adhesion of fibroblasts to collagen , 1976, Nature.

[33]  W. Falk,et al.  Rapid quantitation of neutrophil chemotaxis: use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assembly. , 1980, Journal of immunological methods.

[34]  E. Engvall,et al.  Binding of soluble form of fibroblast surface protein, fibronectin, to collagen , 1977, International journal of cancer.

[35]  C. C. Clark,et al.  Biochemistry and metabolism of basement membranes. , 1979, International review of cytology.

[36]  E. Engvall,et al.  Complexing of fibronectin glycosaminoglycans and collagen. , 1980, Biochimica et biophysica acta.

[37]  E. J. Miller,et al.  Nature of the collagen synthesized by a transplanted chondrosarcoma. , 1975, Archives of biochemistry and biophysics.

[38]  J. A. Weston,et al.  A radioautographic analysis of the migration and localization of trunk neural crest cells in the chick. , 1963, Developmental biology.

[39]  D. Woolley,et al.  Localization of the binding site for cell attachment in the alpha1(I) chain of collagen. , 1978, The Journal of biological chemistry.

[40]  E. Hay,et al.  GLYCOSAMINOGLYCAN SYNTHESIS BY EMBRYONIC INDUCTORS: NEURAL TUBE, NOTOCHORD, AND LENS , 1974, The Journal of cell biology.

[41]  D. Noden.  Interactions Directing the Migration and Cytodifferentiation of Avian Neural Crest Cells , 1978 .

[42]  P. Bornstein,et al.  The nature of the intramolecular cross-links in collagen. The separation and characterization of peptides from the cross-link region of rat skin collagen. , 1966, Biochemistry.

[43]  Kathryn W. Tosney.  The early migration of neural crest cells in the trunk region of the avian embryo: an electron microscopic study. , 1978, Developmental biology.

[44]  R. Pratt,et al.  Glycosaminoglycan and glycoprotein synthesis by cranial neural crest cells in vitro. , 1977, Cell differentiation.

[45]  M. C. Johnston,et al.  CHAPTER 4 – Observations on the Migration, Interaction, and Early Differentiation of Orofacial Tissues , 1972 .

[46]  M. C. Johnston,et al.  Migration of cranial neural crest cells in a cell-free hyaluronate-rich matrix. , 1975, Developmental biology.

[47]  R. Klebe,et al.  Isolation of a collagen-dependent cell attachment factor , 1974, Nature.

[48]  H. Kleinman,et al.  Localization of the cell attachment region in types I and II collagens. , 1976, Biochemical and biophysical research communications.

[49]  Paul Weiss,et al.  Guiding principles in cell locomotion and cell aggregation , 1961 .

[50]  K. Yamada,et al.  Characterization of fibronectin interactions with glycosaminoglycans and identification of active proteolytic fragments. , 1980, The Journal of biological chemistry.

[51]  Sally H. Zigmond,et al.  Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. , 1973 .

[52]  R. Markwald,et al.  A histochemical analysis of polyanionic compounds found in the extracellular matrix encountered by migrating cephalic neural crest cells , 1980 .