Pyrolysis/gas chromatography/mass spectrometry monitoring of fungal-biotreated distillery wastewater using Trametes sp. I-62 (CECT 20197).

Distillery wastewaters generated by ethanol production from fermentation of sugar-cane molasses, named vinasses, lead to important ecological impact due to their high content of soluble organic matter and their intense dark-brown color. Taking advantage of the well-known ability of white-rot fungi to degrade an extensive variety of organic pollutants, the capacity of Trametes sp. I-62 (CECT 20197) to detoxify this type of effluents was evaluated. In this work, pyrolysis/gas chromatography/mass spectrometry was applied to the chemical characterization of several fractions of Cuban distillery wastewater as well as to monitoring the changes which occurred after fungal treatment with this white-rot basidiomycete. Maximum effluent decolorization values and chemical oxygen demand reduction attained after seven days of fungal treatment were 73.3 and 61.7%, respectively, when 20% (v/v) of distillery vinasses was added to the culture medium. Under these conditions a 35-fold increase in laccase production by Trametes sp. I-62 was measured, but no manganese peroxidase activity could be detected. The pyrolysis/gas chromatography/mass spectrometry results showed a decrease in a number of pyrolysis products after seven days of fungal treatment, mainly furan derivatives. The decrease in the relative areas of these compounds could be related to the vinasse color-removal associated with melanoidin degradation. All these results indicated the potential use ofTrametes sp. I-62 in the detoxification of recalcitrant distillery vinasses.

[1]  P. Bocchini,et al.  Biotreatment of tannin-rich beer-factory wastewater with white-rot basidiomycete Coriolopsis gallica monitored by pyrolysis/gas chromatography/mass spectrometry. , 2000, Rapid communications in mass spectrometry : RCM.

[2]  U. Hölker,et al.  Evidence for and expression of a laccase gene in three basidiomycetes degrading humic acids , 1999, Applied Microbiology and Biotechnology.

[3]  M. Hofrichter,et al.  Biotechnology and microbiology of coal degradation , 1999, Applied Microbiology and Biotechnology.

[4]  S. K. Garg,et al.  Decolorization of Pulp-Paper Mill Effluents by White-Rot Fungi , 1999 .

[5]  D. Fabbri,et al.  Comparison between CP/MAS 13C-NMR and pyrolysis-GC/MS in the structural characterization of humins and humic acids of soil and sediments , 1998 .

[6]  A. Mermut,et al.  Carbon-13 CP/MAS NMR and DR-FTIR spectroscopic studies of sugarcane distillery waste , 1998 .

[7]  M. Mansur,et al.  Differential Gene Expression in the Laccase Gene Family from Basidiomycete I-62 (CECT 20197) , 1998, Applied and Environmental Microbiology.

[8]  M. Fujita,et al.  Manganese-independent and -dependent decolorization of melanoidin by extracellular hydrogen peroxide and peroxidases from Coriolus hirsutus pellets , 1998 .

[9]  R. Evershed,et al.  Assessment of bog-body tissue preservation by pyrolysis-gas chromatography/mass spectrometry. , 1997, Rapid communications in mass spectrometry : RCM.

[10]  G. Benito,et al.  Decolorization of wastewater from an alcoholic fermentation process with Trametes versicolor , 1997 .

[11]  M. Mansur,et al.  Identification of a laccase gene family in the new lignin-degrading basidiomycete CECT 20197 , 1997, Applied and environmental microbiology.

[12]  M. Tien,et al.  The Roles of veratryl alcohol and oxalate in fungal lignin degradation , 1997 .

[13]  D. Cullen,et al.  Recent advances on the molecular genetics of ligninolytic fungi. , 1997, Journal of biotechnology.

[14]  Luís Augusto Barbosa Cortez,et al.  EXPERIENCES ON VINASSE DISPOSAL: Part III: COMBUSTION OF VINASSE-# 6 FUEL OIL EMULSIONS , 1997 .

[15]  R Marchant,et al.  Biological treatment of distillery waste for pollution‐remediation , 1995, Journal of basic microbiology.

[16]  A. Hatakka.  Lignin-modifying enzymes from selected white-rot fungi: production and role from in lignin degradation , 1994 .

[17]  C. Thurston.  The structure and function of fungal laccases , 1994 .

[18]  M. L. Fidalgo,et al.  Pyrolysis—gas chromatography/mass spectrometry of wheat straw fractions obtained by alkaline treatments used in pulping processes , 1993 .

[19]  M. L. Fidalgo,et al.  Comparative Study of Fractions from Alkaline Extraction of Wheat Straw through Chemical Degradation, Analytical Pyrolysis, and Spectroscopic Techniques , 1993 .

[20]  M. Gold,et al.  Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium , 1993, Microbiological reviews.

[21]  P. Manzanares,et al.  Pyrolysis/gas chromatography/mass spectrometry of lignin from paper-industry effluents decolorized by Trametes versicolor , 1993 .

[22]  J. C. Río,et al.  The effect of various chemical treatments on the pyrolytic pattern of peat humic acid , 1993 .

[23]  R. Blondeau,et al.  Isolation of an extracellular Mn-dependent enzyme mineralizing melanoidins from the white rot fungus Trametes versicolor , 1993 .

[24]  J. Field,et al.  Screening for ligninolytic fungi applicable to the biodegradation of xenobiotics , 1993 .

[25]  Giuseppe Chiavari,et al.  Pyrolysis—gas chromatography/mass spectrometry of amino acids , 1992 .

[26]  R. Blondeau,et al.  Extracellular enzyme activities during humic acid degradation by the white rot fungi Phanerochaete chrysosporium and Trametes versicolor , 1992 .

[27]  J. Field,et al.  Biodegradation of polycyclic aromatic hydrocarbons by new isolates of white rot fungi , 1992, Applied and environmental microbiology.

[28]  K. Hammel.  Oxidation of aromatic pollutants by lignin-degrading fungi and their extracellular peroxidases , 1992 .

[29]  J. Ralph,et al.  Pyrolysis-GC-MS characterization of forage materials , 1991 .

[30]  F. Archibald,et al.  Direct dechlorination of chlorophenolic compounds by laccases from Trametes (Coriolus) versicolor , 1991 .

[31]  A. Scalbert.  Antimicrobial properties of tannins , 1991 .

[32]  M. Paice,et al.  Decolorization of kraft bleachery effluent chromophores by Coriolus (Trametes) versicolor , 1990 .

[33]  R. Blondeau.  Biodegradation of Natural and Synthetic Humic Acids by the White Rot Fungus Phanerochaete chrysosporium , 1989, Applied and environmental microbiology.

[34]  Gert B. Eijkel,et al.  Curie-point pyrolysis-capillary gas chromatography-high-resolution mass spectrometry of microcrystalline cellulose , 1989 .

[35]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[36]  P. Atthasampunna,et al.  Research on decolorization of melanoidin by microorganisms. Part V. Decolorization of molasses waste water by a thermophilic strain, Aspergillus fumigatus G-2-6. , 1987 .

[37]  M. Tien,et al.  Production of multiple ligninases by Phanerochaete chrysosporium: effect of selected growth conditions and use of a mutant strain , 1986 .

[38]  S. Ohmomo,et al.  Production of decolorizing activity for molasses pigment by Coriolus versicolor Ps4a. , 1985 .

[39]  E. Valdez,et al.  Estudio del tratamiento anaerobico de los residuales de la industria alcoholera , 1985 .

[40]  Yoshio Watanabe,et al.  Enzymatic Decolorization of Melanoidin by Coriolus sp. No. 20 , 1982 .

[41]  R. Willson,et al.  Radical-cations as reference chromogens in kinetic studies of ono-electron transfer reactions: pulse radiolysis studies of 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) , 1982 .

[42]  Paul F. Greenfield,et al.  Utilisation, treatment and disposal of distillery wastewater , 1980 .

[43]  M. Fujimaki,et al.  Chemical Studies on Melanoidins Part III , 1971 .