Raman spectra of crystalline 4Zn, 2Zn, and Na insulin

Normal Raman spectra were obtained for three crystalline forms of human insulin: 4Zn, 2Zn, and Zn-free or Na, from 1800-200 cm1. The extraction of a large number of component bands from the heavily overlapped Raman bands was accomplished by Fourier Self Deconvolution and bandfitting. Bands considered to be indicative of protein conformation, including Amide I, Amide III, tyrosine, 5-5, and C-S bands, and some which are relatively insensitive to protein structure, such as phenylalanine and histidine, are compared. The published x-ray structures of 4Zn and 2Zn insulins are used to help interpret the corresponding parameters of the extracted Raman bands, and to suggest structures in the as yet unpublished Na/human insulin crystals.

[1]  E. Baker,et al.  Structure of Rhombohedral 2 Zinc Insulin Crystals , 1969, Nature.

[2]  P. Tooke,et al.  Fourier self-deconvolution in IR spectroscopy , 1988 .

[3]  E. Dodson,et al.  Zinc-free cubic pig insulin: crystallization and structure determination. , 1978, Journal of molecular biology.

[4]  T. Shimanouchi,et al.  Interpretation of the doublet at 850 and 830 cm-1 in the Raman spectra of tyrosyl residues in proteins and certain model compounds. , 1975, Biochemistry.

[5]  P. Griffiths,et al.  Introduction to spectral deconvolution , 1986 .

[6]  E. Dodson,et al.  Structural stability in the 4-zinc human insulin hexamer. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[7]  N T Yu,et al.  Laser Raman spectra of native and denatured insulin in the solid state. , 1972, Journal of the American Chemical Society.

[8]  L. G. Tensmeyer.  The raman spectrum of biosynthetic human growth hormone. Its deconvolution, bandfitting, and interpretation , 1988 .

[9]  B. Gaber,et al.  Laser Raman scattering from an enzyme of well-documented structure, human carbonic anhydrase B. , 1977, Journal of the American Chemical Society.

[10]  H. Sugeta,et al.  Vibrational Spectra and Molecular Conformations of Dialkyl Disulfides , 1973 .

[11]  Douglas J. Moffatt,et al.  Fourier Self-Deconvolution: A Method for Resolving Intrinsically Overlapped Bands , 1981 .

[12]  D. Mercola,et al.  Structure of insulin in 4-zinc insulin , 1976, Nature.