Novel application of Nerium leaf and Image J software in drop collapse assay for rapid screening of biosurfactant producing microorganisms

Biosurfactants are attractive molecules with varied applicationsmainly oil degradation, emulsification, bioremediation, therapeutics and conjugation of nanoparticles. The existing screening methods for biosurfactants are inappropriate and too tedious. Here, we have explored a novel approach with drop collapse assay wherein we replaced the microtiter well plate with the naturally hydrophobic Nerium ( Nerium oleander L . ) leaf. The stability of beaded drops on the leaf indicates negative phenomenon, and spreading of drop indicates positive phenomenon for surfactant property, as confirmed by the measuring drop diameter using Image J software. Fifty five bacterial cultures isolated from oil contaminated site were screened through this novel approach which revealed that the isolates DNM49 (6.75±0.29 mm), DNM50 (7.45±0.19 mm) and DNM51 (6.14±0.82 mm) were the best in terms of surface tension reduction, although thirty other isolates were also found to be positive. A gradation of activity in terms of surface tension reduction was also established based on drop diameter. The results demonstrated promising application of Nerium leaf with Image J software in drop collapse assay as an eco-friendly and cost-effective and technically authenticated alternative to the existing assays.

[1]  B. Bharathiraja,et al.  Biodegradation of sulfanilic acid using Bacillus cereus AAA2018 from textile industry effluent contaminated soil , 2020, Indian Journal of Experimental Biology.

[2]  Sophie Roelants,et al.  Microbial biosurfactant research: time to improve the rigour in the reporting of synthesis, functional characterization and process development , 2020, Microbial biotechnology.

[3]  Hamidreza Hajfarajollah,et al.  Recent advancements in the production of rhamnolipid biosurfactants by Pseudomonas aeruginosa , 2020, RSC advances.

[4]  Eric Déziel,et al.  Quorum Sensing Controls Both Rhamnolipid and Polyhydroxyalkanoate Production in Burkholderia thailandensis Through ScmR Regulation , 2020, Frontiers in Bioengineering and Biotechnology.

[5]  L. Blank,et al.  A Straightforward Assay for Screening and Quantification of Biosurfactants in Microbial Culture Supernatants , 2020, Frontiers in Bioengineering and Biotechnology.

[6]  G. Płaza,et al.  Biosurfactants: Eco-Friendly and Innovative Biocides against Biocorrosion , 2020, International journal of molecular sciences.

[7]  Yanfen Wang,et al.  Production and characterization of surfactin-like biosurfactant produced by novel strain Bacillus nealsonii S2MT and it's potential for oil contaminated soil remediation , 2020, Microbial Cell Factories.

[8]  D. Kadam,et al.  Biosurfactant production from shrimp shell waste by Pseudomonas stutzeri , 2019 .

[9]  I. Banat,et al.  Microbial biosurfactants: current trends and applications in agricultural and biomedical industries , 2019, Journal of applied microbiology.

[10]  P. Setoodeh,et al.  Biosurfactant Production by Lactic Acid Bacterium Pediococcus dextrinicus SHU1593 Grown on Different Carbon Sources: Strain Screening Followed by Product Characterization , 2019, Scientific Reports.

[11]  Y. Patil,et al.  Biosurfactant production: emerging trends and promising strategies , 2018, Journal of applied microbiology.

[12]  S. Singh,et al.  Screening, isolation and characterization of biosurfactant producing Bacillus subtilis strain ANSKLAB03 , 2018, Bioinformation.

[13]  M. Kamil,et al.  Studies on the interaction between polymer and surfactant in aqueous solutions , 2018 .

[14]  S. R. Ambati.  Dual substrate fermentation using palm oil and glucose for production of eco-friendly biosurfactants using P. aeruginosa NITT 6L , 2018 .

[15]  V. Upasani,et al.  Critical review on biosurfactant analysis, purification and characterization using rhamnolipid as a model biosurfactant. , 2017, Bioresource technology.

[16]  Bharat Bhushan,et al.  Plant Surfaces: Structures and Functions for Biomimetic Innovations , 2017, Nano-Micro Letters.

[17]  Deepansh Sharma,et al.  Functional characterization of biomedical potential of biosurfactant produced by Lactobacillus helveticus , 2016, Biotechnology reports.

[18]  L. Sarubbo,et al.  Production of a Biosurfactant from Candida Bombicola Urm 3718 for Environmental Applications , 2016 .

[19]  Sougata Jana,et al.  Biosurfactant produced from Actinomycetes nocardiopsis A17: Characterization and its biological evaluation. , 2015, International journal of biological macromolecules.

[20]  M. Kalita,et al.  Degradation of polycyclic aromatic hydrocarbons (PAHs) employing biosurfactant producing Pseudomonas aeruginosa KS3 , 2015 .

[21]  Kevin W Eliceiri,et al.  NIH Image to ImageJ: 25 years of image analysis , 2012, Nature Methods.

[22]  I. Banat,et al.  Isolation of biosurfactant-producing Pseudomonas aeruginosa RS29 from oil-contaminated soil and evaluation of different nitrogen sources in biosurfactant production , 2012, Annals of Microbiology.

[23]  Wilhelm Barthlott,et al.  Superhydrophobicity in perfection: the outstanding properties of the lotus leaf , 2011, Beilstein journal of nanotechnology.

[24]  K. Das,et al.  Differential utilization of pyrene as the sole source of carbon by Bacillus subtilis and Pseudomonas aeruginosa strains: role of biosurfactants in enhancing bioavailability , 2007, Journal of applied microbiology.

[25]  I. Banat,et al.  Use of different methods for detection of thermophilic biosurfactant-producing bacteria from hydrocarbon-contaminated and bioremediated soils , 2006 .

[26]  A. Gianguzza,et al.  LEAVES OF NERIUM OLEANDER L. AS BIOACCUMULATORS OF POLYCYCLIC AROMATIC HYDROCARBONS (PAH) IN THE AIR OF PALERMO (ITALY): EXTRACTION AND GC-MS ANALYSIS, DISTRIBUTION AND SOURCES , 2005 .

[27]  N. Youssef,et al.  Comparison of methods to detect biosurfactant production by diverse microorganisms. , 2004, Journal of microbiological methods.

[28]  B. Chopade,et al.  Studies on bioemulsifier production by Acinetobacter strains isolated from healthy human skin , 2001, Journal of applied microbiology.

[29]  T. Imanaka,et al.  A study on the structure-function relationship of lipopeptide biosurfactants. , 2000, Biochimica et biophysica acta.

[30]  A. Bodour,et al.  Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant-producing microorganisms , 1998 .

[31]  I. Banat,et al.  Microbial production of surfactants and their commercial potential , 1997, Microbiology and molecular biology reviews : MMBR.

[32]  K. Percy.  Air Pollutants and the Leaf Cuticle , 1994, NATO ASI Series.

[33]  Fritz Wagner,et al.  New method for detecting rhamnolipids excreted by Pseudomonas species during growth on mineral agar , 1991 .

[34]  D. Cooper,et al.  Surface-Active Agents from Two Bacillus Species , 1987, Applied and environmental microbiology.

[35]  J. Daily,et al.  The use of coomassie brilliant blue for critical micelle concentration determination of detergents , 1986 .

[36]  W. M. Sams,et al.  Human Poisoning From Native and Cultivated Plants , 1971 .

[37]  R. C. Lawrence,et al.  Rapid Method for the Quantitative Estimation of Microbial Lipases , 1967, Nature.

[38]  John Merriam Kingsbury,et al.  Poisonous plants of the United States and Canada , 1964 .

[39]  C. Khobragade,et al.  Production of biosurfactant from Bacillus subtilis (MF 582633) and its evaluation for antimicrobial, antioxidant, larvicidal and antitermite activities , 2019 .

[40]  Christoph Syldatk,et al.  Screening concepts for the isolation of biosurfactant producing microorganisms. , 2010, Advances in experimental medicine and biology.

[41]  B. Lakusic,et al.  Morpho-anatomical characteristics of the raw material of the herbal drug Olivae folium and its counterfeits , 2007 .

[42]  A. Giulietti,et al.  Isolation and selection of biosurfactant-producing bacteria , 1996, World journal of microbiology & biotechnology.

[43]  P. J. Holloway.  Plant Cuticles: Physicochemical Characteristics and Biosynthesis , 1994 .

[44]  C. Mulligan,et al.  Selection of microbes producing biosurfactants in media without hydrocarbons , 1984 .