Introductory neurobiology courses face the problem that practical exercises often require expensive equipment, dissections, and a favorable student-instructor ratio. Furthermore, the duration of an experiment might exceed available time or the level of required expertise is too high to successfully complete the experiment. As a result, neurobiological experiments are commonly replaced by models and simulations, or provide only very basic experiments, such as the frog sciatic nerve preparation, which are often time consuming and tedious. Action potential recordings in giant fibers of intact earthworms (Lumbricus terrestris) circumvent many of these problems and result in a nearly 100% success rate. Originally, these experiments were introduced as classroom exercises by Charles Drewes in 1978 using awake, moving earthworms. In 1990, Hans-Georg Heinzel described further experiments using anesthetized earthworms. In this article, we focus on the application of these experiments as teaching tools for basic neurobiology courses. We describe and extend selected experiments, focusing on specific neurobiological principles with experimental protocols optimized for classroom application. Furthermore, we discuss our experience using these experiments in animal physiology and various neurobiology courses at the University of Bonn.
[1]
Aleksander W. Ballo,et al.
Complex Intrinsic Membrane Properties and Dopamine Shape Spiking Activity in a Motor Axon
,
2009,
The Journal of Neuroscience.
[2]
F. Krasne.
ESCAPE FROM RECURRING TACTILE STIMULATION IN BRANCHIOMMA VESICULOSUM.
,
1965,
The Journal of experimental biology.
[3]
M. Alexander,et al.
Principles of Neural Science
,
1981
.
[4]
S. Schultz.
Principles of Neural Science, 4th ed.
,
2001
.
[5]
C. Drewes,et al.
Giant nerve fibre activity in intact, freely moving earthworms.
,
1978,
The Journal of experimental biology.
[6]
Theodore H. Bullock,et al.
FUNCTIONAL ORGANIZATION OF THE GIANT FIBER SYSTEM OF LUMBRICUS
,
1945
.
[7]
Das Experiment: Neurophysiologische Versuche am intakten Regenwurm. Tierschutz durch Alternativen
,
1990
.
[8]
J. Günther.
Overlapping sensory fields of the giant fiber systems in the earthworm
,
1973,
Naturwissenschaften.
[9]
Giant motor neurons in the earthworm
,
1972
.
[10]
I. K. Wood.
Neuroscience: Exploring the brain
,
1996
.
[11]
J. Young.
Structure and function in the nervous systems of invertebrates
,
1966
.
[12]
T. Bullock.
Facilitation of conduction rate in nerve fibres
,
1951,
The Journal of physiology.