Microbial analysis of a phototrophic sludge producing hydrogen from acidified wastewater

Based on DNA-cloning analysis, the microbial community of a phototrophic sludge producing H2 from an acidified wastewater was composed of 81% of a species resembling Rhodobactercapsulatus (with 99.2% similarity) and two unidentified species of the Bacillus/Clostridium group. The sludge produced a biogas comprising 82 ± 2% H2, 13 ± 2% CO2, 4.5 ± 0.5% N2, and 0.5 ± 0.2% H2S.

[1]  Hong Liu,et al.  Effect of pH on hydrogen production from glucose by a mixed culture. , 2002, Bioresource technology.

[2]  R. Bachofen,et al.  Hydrogen Production by the Photosynthetic Bacterium Rhodospirillum rubrum , 1979, Applied and environmental microbiology.

[3]  T. Matsunaga,et al.  Conversion Efficiencies of Light Energy to Hydrogen by a Novel Rhodovulum sp. and its Uptake-Hydrogenase Mutant , 1998 .

[4]  D. Hall,et al.  Actual and potential rates of hydrogen photoproduction by continuous culture of the purple non-sulphur bacterium Rhodobacter capsulatus , 1998, Applied Microbiology and Biotechnology.

[5]  I. Eroglu,et al.  Continuous Hydrogen Production by Rhodobacter sphaeroides O.U.001 , 1998 .

[6]  E. Fascetti,et al.  Rhodobacter sphaeroides RV cultivation and hydrogen production in a one- and two-stage chemostat , 1995, Applied Microbiology and Biotechnology.

[7]  J. Imhoff.  Anoxygenic phototrophic bacteria , 1988 .

[8]  D. Hall,et al.  The biotechnology of hydrogen production by Nostoc flagelliforme grown under chemostat conditions , 1997, Applied Microbiology and Biotechnology.

[9]  I. Tseng,et al.  Characterization of a 4-methylbenzoate-degrading methanogenic consortium as determined by small-subunit rDNA sequence analysis. , 2001, Journal of bioscience and bioengineering.

[10]  Shigeru Uchiyama,et al.  Hydrogen Production by a Floating-Type Photobioreactor , 1998 .

[11]  Tong Zhang,et al.  Characterization of a hydrogen-producing granular sludge. , 2002, Biotechnology and bioengineering.

[12]  H. Fang,et al.  Phylogenetic diversity of a SRB-rich marine biofilm , 2001, Applied Microbiology and Biotechnology.