Common-path configuration in total internal reflection digital holography microscopy

TIRDHM is a technique that allows to analyse the phase change of microscopical sections produced on the prism surface due to material attached on the top. Therefore, due to the evanescence waves properties we can analyse quantitatively the properties and specific morphology located to few nanometers on the top of surface contact. In this work, we study and present an alternative method to off-axis configuration to record and analyse the microscopical phase object information in Total Internal Reflection dispensing with the use of reference arm.

[1]  D. Axelrod Cell-substrate contacts illuminated by total internal reflection fluorescence , 1981, The Journal of cell biology.

[2]  Jingang Zhong,et al.  Digital holographic microscopy by use of surface plasmon resonance for imaging of cell membranes. , 2010, Journal of biomedical optics.

[3]  Myung K. Kim,et al.  Digital holography of total internal reflection. , 2008, Optics express.

[4]  W. HICKEL,et al.  Surface-plasmon microscopy , 1989, Nature.

[5]  Myung K. Kim Principles and techniques of digital holographic microscopy , 2010 .

[6]  F Guilak,et al.  A method for quantifying cell size from differential interference contrast images: validation and application to osmotically stressed chondrocytes , 2002, Journal of microscopy.

[7]  Chun-Min Lo,et al.  High-resolution quantitative phase-contrast microscopy by digital holography. , 2005, Optics express.

[8]  Melania Paturzo,et al.  Enhancing depth of focus in tilted microfluidics channels by digital holography. , 2013, Optics letters.

[9]  Ichirou Yamaguchi,et al.  Phase-shifting color digital holography. , 2002, Optics letters.

[10]  Werner Jüptner,et al.  Digital recording and numerical reconstruction of holograms , 2002 .

[11]  Myung K. Kim,et al.  Quantitative imaging of cellular adhesion by total internal reflection holographic microscopy. , 2009, Applied optics.

[12]  E. Cuche,et al.  Digital holography for quantitative phase-contrast imaging. , 1999, Optics letters.

[13]  K. Nugent,et al.  Quantitative optical phase microscopy. , 1998, Optics letters.