Improvements in Germination and Growth of Sprouts Irrigated Using Plasma Activated Water (PAW)

The extensive use of chemical fertilizers to increase crop yields in agricultural fields has had a negative impact on the environment. To produce more food on less land and fulfill the growing global demand for food, farmers will need innovative and environmentally friendly technology. Several studies have cited the positive effects of plasma-activated water (PAW) on seeds in their research findings. This study investigates the effects of PAW on four distinct seed species: phapar (Fagopyrum esculentum), barley (Hordeum vulgare), mustard (Brassica nigra), and rayo (Brassica juncea). Deionized (DI) water was treated for 5 or 10 min using the gliding arc discharge (GAD) system, which was operated by line frequency in the air. Water analysis indicates that the physiochemical parameters (electrical conductivity, pH, nitrate, nitrite, and ammonia concentration) of PAW were significantly different from DI water. Despite exposure to GAD for a certain period of time, the temperature of DI water did not alter significantly. All calculated germination parameters were significantly enhanced for seeds treated with PAW compared to the control. In addition, they displayed a significant increase in total seedling length and exhibited greater vigor. Seeds immersed in PAW absorbed significantly more water than seeds soaked in DI water, enabling rapid water penetration into the seed and early seedling emergence. This puts plasma agriculture ahead of conventional farming methods.

[1]  D. Subedi,et al.  Growth Enhancement of Radish Seed Induced by Low-Temperature Argon Plasma , 2022, Plasma Chemistry and Plasma Processing.

[2]  C. Gyuricza,et al.  The Effects of Temperature and Water on the Seed Germination and Seedling Development of Rapeseed (Brassica napus L.) , 2022, Plants.

[3]  Jun‐Hu Cheng,et al.  Enhancement of Wheat Seed Germination, Seedling Growth and Nutritional Properties of Wheat Plantlet Juice by Plasma Activated Water , 2022, Journal of Plant Growth Regulation.

[4]  B. Šerá,et al.  Comparison of the Effect of Plasma-Activated Water and Artificially Prepared Plasma-Activated Water on Wheat Grain Properties , 2022, Plants.

[5]  S. Park,et al.  Current Advancements in the Molecular Mechanism of Plasma Treatment for Seed Germination and Plant Growth , 2022, International journal of molecular sciences.

[6]  A. Luch,et al.  Emerging cold plasma treatment and machine learning prospects for seed priming: a step towards sustainable food production , 2022, RSC advances.

[7]  D. Baniulis,et al.  Biochemical and Physiological Plant Processes Affected by Seed Treatment with Non-Thermal Plasma , 2022, Plants.

[8]  Tilahun Gisila Abebe,et al.  Growing Use and Impacts of Chemical Fertilizers and Assessing Alternative Organic Fertilizer Sources in Ethiopia , 2022, Applied and Environmental Soil Science.

[9]  M. A. Bashir,et al.  Pesticides Xenobiotics in Soil Ecosystem and Their Remediation Approaches , 2022, Sustainability.

[10]  Xianfeng Tan,et al.  Effects of Agriculture and Animal Husbandry on Heavy Metal Contamination in the Aquatic Environment and Human Health in Huangshui River Basin , 2022, Water.

[11]  Md. Abu Reza,et al.  Low-frequency glow discharge (LFGD) plasma treatment enhances maize (Zea mays L.) seed germination, agronomic traits, enzymatic activities, and nutritional properties , 2022, Chemical and Biological Technologies in Agriculture.

[12]  S. Kocira,et al.  Cold Plasma as a Potential Activator of Plant Biostimulants , 2022, Sustainability.

[13]  D. Subedi,et al.  Impact of Plasma-Activated Water (PAW) on Seed Germination of Soybean , 2021, Journal of Chemistry.

[14]  D. Subedi,et al.  Impact of non-thermal plasma treatment on the seed germination and seedling development of carrot (Daucus carota sativus L.) , 2021, Journal of Physics Communications.

[15]  D. Subedi,et al.  Influence of plasma-activated water (PAW) on the germination of radish, fenugreek, and pea seeds , 2021, AIP Advances.

[16]  M. Dabalà,et al.  Plasma-Activated Water Triggers Rapid and Sustained Cytosolic Ca2+ Elevations in Arabidopsis thaliana , 2021, Plants.

[17]  D. Subedi,et al.  Effect of plasma treatment on the seed germination and seedling growth of radish (Raphanus sativus) , 2021, Plasma Science and Technology.

[18]  B. S. Tiwari,et al.  Treatment of Pea Seeds with Plasma Activated Water to Enhance Germination, Plant Growth, and Plant Composition , 2021, Plasma Chemistry and Plasma Processing.

[19]  N. Joshi,et al.  Cold plasma technology: advanced and sustainable approach for wastewater treatment , 2021, Environmental Science and Pollution Research.

[20]  A. Zahoranová,et al.  Decontamination of Escherichia coli on the surface of soybean seeds using plasma activated water , 2021, LWT.

[21]  Z. Hochman,et al.  The future of farming: Who will produce our food? , 2021, Food Security.

[22]  M. Kopacki,et al.  Influence of Plasma Activated Water Generated in a Gliding Arc Discharge Reactor on Germination of Beetroot and Carrot Seeds , 2021, Applied Sciences.

[23]  Xiangyou Wang,et al.  Properties of plasma-activated water with different activation time and its effects on the quality of button mushrooms (Agaricus bisporus) , 2021, LWT.

[24]  K. Thumanu,et al.  Development of a Multihole Atmospheric Plasma Jet for Growth Rate Enhancement of Broccoli Seeds , 2021, Processes.

[25]  M. Mozetič,et al.  Hydrophilization of corn seeds by non-equilibrium gaseous plasma , 2021, Chemical and Biological Technologies in Agriculture.

[26]  Lei Zhou,et al.  Plasma-activated water production and its application in agriculture. , 2021, Journal of the science of food and agriculture.

[27]  A. Howling,et al.  Mechanisms of Plasma-Seed Treatments as a Potential Seed Processing Technology , 2021, Frontiers in Physics.

[28]  Ram Pandit,et al.  Pathways for building resilience to COVID-19 pandemic and revitalizing the Nepalese agriculture sector , 2021 .

[29]  A. Gailīte,et al.  Variability of Seed Germination and Dormancy Characteristics and Genetic Analysis of Latvian Avena fatua Populations , 2021, Plants.

[30]  Jonghyun Choi,et al.  Plasma-Activated Water (PAW) as a Disinfection Technology for Bacterial Inactivation with a Focus on Fruit and Vegetables , 2021, Foods.

[31]  A. Kamili,et al.  Chemical Fertilizers and Their Impact on Soil Health , 2021 .

[32]  A. Asfaw,et al.  Seed Viability, Seedling Growth and Yield in White Guinea Yam , 2020, Agronomy.

[33]  Parviz Moradi,et al.  Germination and Seedling Growth Responses of Zygophyllum fabago, Salsola kali L. and Atriplex canescens to PEG-Induced Drought Stress , 2020, Environments.

[34]  G. Mihalache,et al.  Plant growth promotion effect of plasma activated water on Lactuca sativa L. cultivated in two different volumes of substrate , 2020, Scientific Reports.

[35]  F. Krčma,et al.  Influence of Plasma-Activated Water on Physical and Physical–Chemical Soil Properties , 2020 .

[36]  R. Molina,et al.  Physicochemical surface analysis and germination at different irrigation conditions of DBD plasma‐treated wheat seeds , 2020, Plasma Processes and Polymers.

[37]  P. Joshi,et al.  Adoption and diffusion of improved technologies and production practices in agriculture: Insights from a donor-led intervention in Nepal , 2020, Land Use Policy.

[38]  K. Kutasi,et al.  Reactive nitrogen species in plasma-activated water: generation, chemistry and application in agriculture , 2020, Journal of Physics D: Applied Physics.

[39]  Peiyu Wang,et al.  Plasma-activated water: generation, origin of reactive species and biological applications , 2020, Journal of Physics D: Applied Physics.

[40]  G. Velthof,et al.  How Can Decision Support Tools Help Reduce Nitrate and Pesticide Pollution from Agriculture? A Literature Review and Practical Insights from the EU FAIRWAY Project , 2020, Water.

[41]  Liumin Fan,et al.  Effects of plasma-activated water treatment on seed germination and growth of mung bean sprouts , 2020, Journal of Taibah University for Science.

[42]  N. C. Roy,et al.  Plasma activated water: the next generation eco-friendly stimulant for enhancing plant seed germination, vigor and increased enzyme activity, a study on black gram (Vigna mungo L.) , 2019, Plasma Chemistry and Plasma Processing.

[43]  K. Lotfy,et al.  Cold Atmospheric Pressure Nitrogen Plasma Jet for Enhancement Germination of Wheat Seeds , 2019, Plasma Chemistry and Plasma Processing.

[44]  Cuncang Jiang,et al.  Biochar Induces Changes to Basic Soil Properties and Bacterial Communities of Different Soils to Varying Degrees at 25 mm Rainfall: More Effective on Acidic Soils , 2019, Front. Microbiol..

[45]  Xianhui Zhang,et al.  Atmospheric-pressure plasma treated water for seed germination and seedling growth of mung bean and its sterilization effect on mung bean sprouts , 2019, Innovative Food Science & Emerging Technologies.

[46]  P. Lukeš,et al.  On the Possibilities of Straightforward Characterization of Plasma Activated Water , 2019, Plasma Chemistry and Plasma Processing.

[47]  A. Álvarez‐Ordoñez,et al.  A Review on Non-thermal Atmospheric Plasma for Food Preservation: Mode of Action, Determinants of Effectiveness, and Applications , 2019, Front. Microbiol..

[48]  L. Prevosto,et al.  Improvement of growth and yield of soybean plants through the application of non-thermal plasmas to seeds with different health status , 2019, Heliyon.

[49]  K. Hensel,et al.  Effects of plasma activated water on wheat: Germination, growth parameters, photosynthetic pigments, soluble protein content, and antioxidant enzymes activity , 2019, Plasma Processes and Polymers.

[50]  J. Opoku,et al.  Effect of Pre-Sowing Treatments on Seed Germination and Seedling Growth of Silver Butterfly Tree (Bauhinia rufescens) , 2018, Current Agriculture Research Journal.

[51]  D. Graves,et al.  Plasma Activated Organic Fertilizer , 2018, Plasma Chemistry and Plasma Processing.

[52]  Marion W. Dixon.  Chemical fertilizer in transformations in world agriculture and the state system, 1870 to interwar period , 2018 .

[53]  B. Shokri,et al.  A study of the effect of gliding arc non-thermal plasma on almonds decontamination , 2018, AIP Advances.

[54]  N. C. Roy,et al.  Effect of pressure on the properties and species production in gliding arc Ar, O 2 , and air discharge plasmas , 2018, Physics of Plasmas.

[55]  K. Siliveru,et al.  Plasma activated water (PAW): Chemistry, physico-chemical properties, applications in food and agriculture , 2018, Trends in Food Science & Technology.

[56]  P. Tuan,et al.  Molecular Mechanisms Underlying Abscisic Acid/Gibberellin Balance in the Control of Seed Dormancy and Germination in Cereals , 2018, Front. Plant Sci..

[57]  F. Saadati,et al.  Comparison of Direct and Indirect cold atmospheric-pressure plasma methods in the B16F10 melanoma cancer cells treatment , 2018, Scientific reports.

[58]  U. Khanal.  Why are farmers keeping cultivatable lands fallow even though there is food scarcity in Nepal? , 2018, Food Security.

[59]  Daxing Wen,et al.  Rapid evaluation of seed vigor by the absolute content of protein in seed within the same crop , 2018, Scientific Reports.

[60]  E. Choi,et al.  The effect of the gap distance between an atmospheric-pressure plasma jet nozzle and liquid surface on OH and N2 species concentrations , 2017 .

[61]  A. Rousseau,et al.  Promoting lentil germination and stem growth by plasma activated tap water, demineralized water and liquid fertilizer , 2017, 1707.08242.

[62]  Ronny Brandenburg,et al.  Dielectric barrier discharges: progress on plasma sources and on the understanding of regimes and single filaments , 2017 .

[63]  L. Hein,et al.  Non-thermal atmospheric pressure plasma jet applied to inactivation of different microorganisms , 2017 .

[64]  Ahmed Khacef,et al.  Enhanced seed germination and plant growth by atmospheric pressure cold air plasma: combined effect of seed and water treatment , 2017 .

[65]  G. Leubner-Metzger,et al.  The biomechanics of seed germination. , 2016, Journal of experimental botany.

[66]  Jrr Verlet,et al.  Plasma–liquid interactions: a review and roadmap , 2016 .

[67]  G. Bassel,et al.  Seed vigour and crop establishment: extending performance beyond adaptation. , 2016, Journal of experimental botany.

[68]  S. S. Singh,et al.  Correlation Dynamics of Germination Value , Germination Energy Index and Germination Speed of Pongamia Pinnata ( L . ) Pierre Seeds of Pendra Provenance , Chhattisgarh , India , 2016 .

[69]  Daniela Hudecová,et al.  Effect of Cold Atmospheric Pressure Plasma on the Wheat Seedlings Vigor and on the Inactivation of Microorganisms on the Seeds Surface , 2016, Plasma Chemistry and Plasma Processing.

[70]  J. Brisset,et al.  Chemical Effects of Air Plasma Species on Aqueous Solutes in Direct and Delayed Exposure Modes: Discharge, Post-discharge and Plasma Activated Water , 2016, Plasma Chemistry and Plasma Processing.

[71]  R. Banerjee,et al.  Seed birth to death: dual functions of reactive oxygen species in seed physiology. , 2015, Annals of botany.

[72]  L. Randeniya,et al.  Non-Thermal Plasma Treatment of Agricultural Seeds for Stimulation of Germination, Removal of Surface Contamination and Other Benefits: A Review , 2015 .

[73]  F. Graef,et al.  Methods to assess farming sustainability in developing countries. A review , 2015, Agronomy for Sustainable Development.

[74]  Ji Hoon Park,et al.  Generation mechanism of hydroxyl radical species and its lifetime prediction during the plasma-initiated ultraviolet (UV) photolysis , 2015, Scientific Reports.

[75]  Junbao Yu,et al.  Responses of Seed Germination, Seedling Growth, and Seed Yield Traits to Seed Pretreatment in Maize (Zea mays L.) , 2014, TheScientificWorldJournal.

[76]  A. Sarani,et al.  Optical emission spectroscopic diagnostics of a non-thermal atmospheric pressure helium-oxygen plasma jet for biomedical applications , 2013 .

[77]  M. Shariati,et al.  Effects of hydropriming on seed germination and seedling growth in sage (Salvia officinalis L.) , 2013 .

[78]  E. Bormashenko,et al.  Cold Radiofrequency Plasma Treatment Modifies Wettability and Germination Speed of Plant Seeds , 2012, Scientific Reports.

[79]  S. Savcı.  Investigation of Effect of Chemical Fertilizers on Environment , 2012 .

[80]  D. G. Santana,et al.  Calculating germination measurements and organizing spreadsheets , 2009 .

[81]  K. Cen,et al.  Dynamic and spectroscopic characteristics of atmospheric gliding arc in gas-liquid two-phase flow , 2009 .

[82]  Bian Wenjuan,et al.  Nitrogen Fixation into HNO3 and HNO2 by Pulsed High Voltage Discharge , 2007 .

[83]  Denise Garcia de Santana,et al.  How and why to measure the germination process , 2006 .

[84]  Sangbo Han,et al.  The Effect of Conductivity on Active Species Products by Electrical Discharge with Air Bubbles in Water , 2005 .

[85]  T. Orchard.  Estimating the parameters of plant seedling emergence , 1977 .

[86]  F. J. Czabator.  Germination Value: An Index Combining Speed and Completeness of Pine Seed Germination , 1962 .