Fluorescence-based Sensing of 2,4,6-Trinitrotoluene (TNT) Using a Multi-channeled Poly(methyl methacrylate) (PMMA) Microimmunosensor

Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene (TNT) were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly (methyl methacrylate) (PMMA) via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene (AlexaFluor-Cad-TNB) was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1–10 ng/mL (ppb) with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine (RDX), HMX, 2,4-dinitrotoluene (DNT), 4-nitrotoluene (4-NT) and 2-amino-4,6-DNT.

[1]  Joseph Wang,et al.  On-line electrochemical monitoring of (TNT) 2,4,6-trinitrotoluene in natural waters , 2003 .

[2]  Richard G. Smith,et al.  A review of biosensors and biologically-inspired systems for explosives detection. , 2008, The Analyst.

[3]  Xiaojuan Fu,et al.  Remote underwater electrochemical sensing system for detecting explosive residues in the field , 2005 .

[4]  Alan R. Kennedy,et al.  The crystal structures of three primary products from the selective reduction of 2,4,6-trinitrotoluene , 2004 .

[5]  James R. Clark,et al.  The toxic effects of trinitrotoluene (TNT) and its primary degradation products on two species of algae and the fathead minnow , 1976 .

[6]  Joseph Wang,et al.  Carbon nanotube-modified glassy carbon electrode for adsorptive stripping voltammetric detection of ultratrace levels of 2,4,6-trinitrotoluene , 2004 .

[7]  Paul T. Charlesa,et al.  Microcapillary reversed-displacement immunosensor for trace level detection of TNT in seawater , 2004 .

[8]  Frances S. Ligler,et al.  Capillary-Based Displacement Flow Immunosensor , 1997 .

[9]  Kiyoshi Toko,et al.  Development of an oligo(ethylene glycol)-based SPR immunosensor for TNT detection. , 2008, Biosensors & bioelectronics.

[10]  U. Narang,et al.  Multianalyte detection using a capillary-based flow immunosensor. , 1998, Analytical biochemistry.

[11]  Anne W. Kusterbeck,et al.  Electrochemical Detection of 2,4,6-Trinitrotoluene Using Interdigitated Array Electrodes , 2008 .

[12]  Victoria L McGuffin,et al.  Luminescence-based methods for sensing and detection of explosives , 2008, Analytical and bioanalytical chemistry.

[13]  Suman Singh,et al.  Sensors--an effective approach for the detection of explosives. , 2007, Journal of hazardous materials.

[14]  Hamid Saberi,et al.  Steric Factors in the Preparation of Nitrostilbenes , 2004 .

[15]  Qin Lu,et al.  Sensitive capillary electrophoresis microchip determination of trinitroaromatic explosives in nonaqueous electrolyte following solid phase extraction , 2002 .

[16]  Igor L. Medintz,et al.  TNT detection using multiplexed liquid array displacement immunoassays. , 2006, Analytical chemistry.

[17]  Laurie Brown,et al.  Fabrication and characterization of poly(methylmethacrylate) microfluidic devices bonded using surface modifications and solvents. , 2006, Lab on a chip.

[18]  L. M. Pant,et al.  The structure of p‐nitrotoluene , 1971 .

[19]  Anne W. Kusterbeck,et al.  TNT Displacement Immunoassay with Integrated Microfluidic Micromixer Components , 2008 .

[20]  Kiyoshi Toko,et al.  High-performance Surface Plasmon Resonance Immunosensors for TNT Detection , 2007 .

[21]  G. Anderson,et al.  Development of a Luminex based competitive immunoassay for 2,4,6-trinitrotoluene (TNT). , 2007, Environmental science & technology.

[22]  Yolanda Y. Davidson,et al.  Surface modification of poly(methyl methacrylate) used in the fabrication of microanalytical devices. , 2000, Analytical chemistry.

[23]  Frances S Ligler,et al.  Fabrication of a capillary immunosensor in polymethyl methacrylate. , 2002, Biosensors & bioelectronics.

[24]  G. Anderson,et al.  Detection of 2,4,6-trinitrotoluene in seawater using a reversed-displacement immunosensor. , 2002, Analytical biochemistry.

[25]  Norges Handelshøyskole,et al.  Structure , 2004, Forum Non Conveniens in the Modern Age: A Comparative and Methodological Analysis of Anglo-American Law.

[26]  A. Kusterbeck,et al.  Trace level detection of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by microimmunosensor. , 1999, Biosensors & bioelectronics.

[27]  Alan R. Kennedy,et al.  Polymorphism in 2-4-6 trinitrotoluene , 2003 .

[28]  Paul I. Okagbare,et al.  Highly efficient circulating tumor cell isolation from whole blood and label-free enumeration using polymer-based microfluidics with an integrated conductivity sensor. , 2008, Journal of the American Chemical Society.

[29]  Patrick Hakey,et al.  Redetermination of cyclo-trimethylenetrinitramine , 2008, Acta crystallographica. Section E, Structure reports online.

[30]  Upvan Narang,et al.  A displacement flow immunosensor for explosive detection using microcapillaries , 1997 .

[31]  Kiyoshi Toko,et al.  Development and comparison of two immunoassays for the detection of 2,4,6-trinitrotoluene (TNT) based on surface plasmon resonance , 2006 .

[32]  C. Choi,et al.  A study of the crystal structure of β‐cyclotetramethylene tetranitramine by neutron diffraction , 1970 .