Shrimp oil extracted from the shrimp processing waste reduces the development of insulin resistance and metabolic phenotypes in diet-induced obese rats.

Diet-induced obesity, insulin resistance, impaired glucose tolerance, chronic inflammation, and oxidative stress represent the main features of type 2 diabetes mellitus. The present study was conducted to examine the efficacy and mechanisms of shrimp oil on glucose homeostasis in obese rats. Male CD rats fed a high-fat diet (52 kcal% fat) and 20% fructose drinking water were divided into 4 groups and treated with the dietary replacement of 0%, 10%, 15%, or 20% of lard with shrimp oil for 10 weeks. Age-matched rats fed a low-fat diet (10 kcal% fat) were used as the normal control. Rats on the high-fat diet showed impaired (p < 0.05) glucose tolerance and insulin resistance compared with rats fed the low-fat diet. Shrimp oil improved (p < 0.05) oral glucose tolerance, insulin response, and homeostatic model assessment-estimated insulin resistance index; decreased serum insulin, leptin, hemoglobin A1c, and free fatty acids; and increased adiponectin. Shrimp oil also increased (p < 0.05) antioxidant capacity and reduced oxidative stress and chronic inflammation. The results demonstrated that shrimp oil dose-dependently improved glycemic control in obese rats through multiple mechanisms.

[1]  R. Considine,et al.  Serum immunoreactive-leptin concentrations in normal-weight and obese humans. , 1996, The New England journal of medicine.

[2]  John C Peters,et al.  Energy balance and obesity. , 2012, Circulation.

[3]  J. Nettleton,et al.  n-3 long-chain polyunsaturated fatty acids in type 2 diabetes: a review. , 2005, Journal of the American Dietetic Association.

[4]  D. Mozaffarian,et al.  Effects of Saturated Fat, Polyunsaturated Fat, Monounsaturated Fat, and Carbohydrate on Glucose-Insulin Homeostasis: A Systematic Review and Meta-analysis of Randomised Controlled Feeding Trials , 2016, PLoS medicine.

[5]  Ahmad Esmaillzadeh,et al.  Effects of omega-3 fatty acid supplementation on insulin metabolism and lipid profiles in gestational diabetes: Randomized, double-blind, placebo-controlled trial. , 2015, Clinical nutrition.

[6]  Walter C Willett,et al.  Dietary fats and prevention of type 2 diabetes. , 2009, Progress in lipid research.

[7]  C. Madigan,et al.  Dietary unsaturated fatty acids in type 2 diabetes: higher levels of postprandial lipoprotein on a linoleic acid-rich sunflower oil diet compared with an oleic acid-rich olive oil diet. , 2000, Diabetes care.

[8]  K. He,et al.  Fish Consumption, Long-Chain Omega-3 Polyunsaturated Fatty Acid Intake and Risk of Metabolic Syndrome: A Meta-Analysis , 2015, Nutrients.

[9]  L. Vitetta,et al.  Omega-3 fatty acids: a review of the effects on adiponectin and leptin and potential implications for obesity management , 2013, European Journal of Clinical Nutrition.

[10]  M. J. Yoon,et al.  Adiponectin Increases Fatty Acid Oxidation in Skeletal Muscle Cells by Sequential Activation of AMP-Activated Protein Kinase, p38 Mitogen-Activated Protein Kinase, and Peroxisome Proliferator–Activated Receptor α , 2006, Diabetes.

[11]  Danielle Swanson,et al.  Omega-3 fatty acids EPA and DHA: health benefits throughout life. , 2012, Advances in nutrition.

[12]  P. Calder,et al.  Inhibition of tumour necrosis factor-alpha and interleukin 6 production by mononuclear cells following dietary fish-oil supplementation in healthy men and response to antioxidant co-supplementation. , 2003, The British journal of nutrition.

[13]  R. Valdés-Ramos,et al.  Vitamins and Type 2 Diabetes Mellitus , 2014, Endocrine, metabolic & immune disorders drug targets.

[14]  R. Heshmat,et al.  Omega-3 fatty acids’ effect on leptin and adiponectin concentrations in patients with spinal cord injury: A double-blinded randomized clinical trial , 2015, The journal of spinal cord medicine.

[15]  S. Sharp,et al.  Association Between Type of Dietary Fish and Seafood Intake and the Risk of Incident Type 2 Diabetes , 2009, Diabetes Care.

[16]  Gokare A. Ravishankar,et al.  Astaxanthin: Sources, Extraction, Stability, Biological Activities and Its Commercial Applications—A Review , 2014, Marine drugs.

[17]  M. Garg,et al.  Higher omega-3 index is associated with increased insulin sensitivity and more favourable metabolic profile in middle-aged overweight men , 2014, Scientific Reports.

[18]  Gb Pant.  Surrogate markers of insulin resistance:A review , 2010 .

[19]  G. Wilcox.  Insulin and insulin resistance. , 2005, The Clinical biochemist. Reviews.

[20]  M. Kiely,et al.  Beneficial effects of long-chain n-3 fatty acids included in an energy-restricted diet on insulin resistance in overweight and obese European young adults , 2008, Diabetologia.

[21]  R. Wu,et al.  Medium-chain triglyceride ameliorates insulin resistance and inflammation in high fat diet-induced obese mice , 2016, European Journal of Nutrition.

[22]  E. Elvevoll,et al.  Health benefits of marine foods and ingredients. , 2011, Biotechnology advances.

[23]  Myung‐Sook Choi,et al.  Obesity and Its Metabolic Complications: The Role of Adipokines and the Relationship between Obesity, Inflammation, Insulin Resistance, Dyslipidemia and Nonalcoholic Fatty Liver Disease , 2014, International journal of molecular sciences.

[24]  Junzeng Zhang,et al.  Ethanol extract of Liuwei Dihuang reduces weight gain and visceral fat in obese-prone CD rats fed a high-fat diet , 2014, Experimental biology and medicine.

[25]  N. Kalupahana,et al.  Health benefits of n-3 polyunsaturated fatty acids: eicosapentaenoic acid and docosahexaenoic acid. , 2012, Advances in food and nutrition research.

[26]  K. Ghose,et al.  Flaxseed lignan secoisolariciresinol diglucoside improves insulin sensitivity through upregulation of GLUT4 expression in diet-induced obese mice , 2015 .

[27]  F. Azizi,et al.  Functional foods-based diet as a novel dietary approach for management of type 2 diabetes and its complications: A review. , 2014, World journal of diabetes.

[28]  M. Nasri,et al.  Astaxanthin from shrimp by-products ameliorates nephropathy in diabetic rats , 2015, European Journal of Nutrition.

[29]  J. D. del Campo,et al.  Accumulation of astaxanthin and lutein in Chlorella zofingiensis (Chlorophyta) , 2004, Applied Microbiology and Biotechnology.

[30]  J. Coombes,et al.  Astaxanthin: A Potential Therapeutic Agent in Cardiovascular Disease , 2011, Marine drugs.

[31]  Naoki Hirose,et al.  Predicting efficacy of dipeptidyl peptidase‐4 inhibitors in patients with type 2 diabetes: Association of glycated hemoglobin reduction with serum eicosapentaenoic acid and docosahexaenoic acid levels , 2012, Journal of diabetes investigation.

[32]  Takahisa Yamada,et al.  Gender Differences in Metabolic Disorders and Related Diseases in Spontaneously Diabetic Torii-Lepr fa Rats , 2014, Journal of diabetes research.

[33]  M. Fernández,et al.  Mechanisms by which dietary fatty acids modulate plasma lipids. , 2005, The Journal of nutrition.

[34]  V. Karathanos,et al.  Controlling type-2 diabetes by inclusion of Cr-enriched yeast bread in the daily dietary pattern: a randomized clinical trial , 2018, European Journal of Nutrition.

[35]  L. Goya,et al.  Cocoa intake ameliorates hepatic oxidative stress in young Zucker diabetic fatty rats , 2015 .

[36]  Farzana Mahdi,et al.  The role of vitamin e in human health and some diseases. , 2014, Sultan Qaboos University medical journal.

[37]  M. Mattson,et al.  Diet‐induced insulin resistance impairs hippocampal synaptic plasticity and cognition in middle‐aged rats , 2008, Hippocampus.

[38]  C. Calhau,et al.  High-fat diet-induced obesity Rat model: a comparison between Wistar and Sprague-Dawley Rat , 2016, Adipocyte.

[39]  W. Pryor.  The antioxidant nutrients and disease prevention--what do we know and what do we need to find out? , 1991, The American journal of clinical nutrition.

[40]  J. Jakicic.  The Effect of Physical Activity on Body Weight , 2009, Obesity.

[41]  B. Popkin,et al.  Global nutrition transition and the pandemic of obesity in developing countries. , 2012, Nutrition reviews.

[42]  R. Turner,et al.  Homeostasis model assessment: insulin resistance and β-cell function from fasting plasma glucose and insulin concentrations in man , 1985, Diabetologia.

[43]  F. Mauvais-Jarvis.  Sex differences in metabolic homeostasis, diabetes, and obesity , 2015, Biology of Sex Differences.

[44]  K. Wellen,et al.  Inflammation, stress, and diabetes. , 2005, The Journal of clinical investigation.

[45]  J. Paul,et al.  Long-chain (n-3) polyunsaturated fatty acids prevent metabolic and vascular disorders in fructose-fed rats. , 2008, The Journal of nutrition.

[46]  Y. Lombardo,et al.  Effects of dietary polyunsaturated n-3 fatty acids on dyslipidemia and insulin resistance in rodents and humans. A review. , 2006, The Journal of nutritional biochemistry.

[47]  秋山 俊治.  High-fat hypercaloric diet induces obesity, glucose intolerance and hyperlipidemia in normal adult male Wistar rat , 1996 .

[48]  Munehiro Yoshida,et al.  Seafood Consumption and Components for Health , 2012, Global journal of health science.

[49]  Shery Jacob,et al.  A simple practice guide for dose conversion between animals and human , 2016, Journal of basic and clinical pharmacy.

[50]  E. Barrett,et al.  Effects of antioxidant supplementation on insulin sensitivity, endothelial adhesion molecules, and oxidative stress in normal-weight and overweight young adults. , 2009, Metabolism: clinical and experimental.

[51]  R. Ross.  Does exercise without weight loss improve insulin sensitivity? , 2003, Diabetes care.

[52]  L. Makowski,et al.  The inflammation highway: metabolism accelerates inflammatory traffic in obesity , 2012, Immunological reviews.

[53]  M. Rewers,et al.  A1C Between 5.7 and 6.4% as a Marker for Identifying Pre-Diabetes, Insulin Sensitivity and Secretion, and Cardiovascular Risk Factors , 2010, Diabetes Care.

[54]  Feng Chen,et al.  Identification of astaxanthin isomers in Haematococcus lacustrisby HPLC-photodiode array detection , 1997 .

[55]  Nancy C. Free,et al.  Effect of long-term fish oil supplementation on vitamin E status and lipid peroxidation in women. , 1991, The Journal of nutrition.

[56]  Yanwen Wang,et al.  Berberine decreases cholesterol levels in rats through multiple mechanisms, including inhibition of cholesterol absorption. , 2014, Metabolism: clinical and experimental.

[57]  M. Chiang,et al.  The effect of high‐fat and high‐fructose diets on glucose tolerance and plasma lipid and leptin levels in rats , 2004, Diabetes, obesity & metabolism.

[58]  R. Vonk,et al.  Dietary fish oil-induced changes in intrahepatic cholesterol transport and bile acid synthesis in rats. , 1991, The Journal of clinical investigation.

[59]  J. Bland,et al.  KDT501, a Derivative from Hops, Normalizes Glucose Metabolism and Body Weight in Rodent Models of Diabetes , 2014, PloS one.

[60]  Y. Terauchi,et al.  The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity , 2001, Nature Medicine.

[61]  Yong Li,et al.  Short- and Long-Term Effects of Wholegrain Oat Intake on Weight Management and Glucolipid Metabolism in Overweight Type-2 Diabetics: A Randomized Control Trial , 2016, Nutrients.

[62]  Y. Djaoued,et al.  Chromatographic, NMR and vibrational spectroscopic investigations of astaxanthin esters: application to "Astaxanthin-rich shrimp oil" obtained from processing of Nordic shrimps. , 2015, The Analyst.

[63]  R. Abbott,et al.  Intake of fish and n-3 fatty acids and future risk of metabolic syndrome. , 2010, Journal of the American Dietetic Association.

[64]  Young-In Kwon,et al.  Effect of Long-Term Dietary Arginyl-Fructose (AF) on Hyperglycemia and HbA1c in Diabetic db/db Mice , 2014, International journal of molecular sciences.

[65]  L. Avigliano,et al.  Obesity-Associated Oxidative Stress: Strategies Finalized to Improve Redox State , 2013, International journal of molecular sciences.

[66]  L. M. Williams,et al.  High‐Fat Diet Induces Leptin Resistance in Leptin‐Deficient Mice , 2014, Journal of neuroendocrinology.