Microglia specific fluorescent probes for live cell imaging.

Small molecule fluorescent probes offer significant advantages over conventional antibody and fluorescent protein labeling techniques. Here we present and , dyes that label live microglia specifically. They may be applied to the isolation and imaging of live microglia when investigating their role in neuroinflammatory diseases.

[1]  Sung-Jin Park,et al.  Visualization and isolation of Langerhans islets by a fluorescent probe PiY. , 2013, Angewandte Chemie.

[2]  Nam-Young Kang,et al.  Neural stem cell specific fluorescent chemical probe binding to FABP7 , 2012, Proceedings of the National Academy of Sciences.

[3]  Sung-Jin Park,et al.  Development of a fluorescent chalcone library and its application in the discovery of a mouse embryonic stem cell probe. , 2012, Chemical communications.

[4]  Nam-Young Kang,et al.  Solid phase combinatorial synthesis of a xanthone library using click chemistry and its application to an embryonic stem cell probe. , 2011, Chemical communications.

[5]  Paul A. Clemons,et al.  The binding of fluorophores to proteins depends on the cellular environment. , 2011, Angewandte Chemie.

[6]  F. Ginhoux,et al.  Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages , 2010, Science.

[7]  Jun Li,et al.  A fluorescent rosamine compound selectively stains pluripotent stem cells. , 2010, Angewandte Chemie.

[8]  K. Ravichandran,et al.  Phagosome maturation: going through the acid test , 2008, Nature Reviews Molecular Cell Biology.

[9]  U. Pal,et al.  A Simplified Method for Isolating Highly Purified Neurons, Oligodendrocytes, Astrocytes, and Microglia from the Same Human Fetal Brain Tissue , 2007, Neurochemical Research.

[10]  Stephen R. Clark,et al.  A Requirement for Microglial TLR4 in Leukocyte Recruitment into Brain in Response to Lipopolysaccharide , 2006, The Journal of Immunology.

[11]  K. Duff,et al.  Transgenic mouse models of Alzheimer's disease: how useful have they been for therapeutic development? , 2004, Briefings in functional genomics & proteomics.

[12]  J. Serratosa,et al.  High‐yield isolation of murine microglia by mild trypsinization , 2003, Glia.

[13]  M. Dailey,et al.  Dynamics of microglial activation: A confocal time‐lapse analysis in hippocampal slices , 2001, Glia.

[14]  A. Sher,et al.  Analysis of Fractalkine Receptor CX3CR1 Function by Targeted Deletion and Green Fluorescent Protein Reporter Gene Insertion , 2000, Molecular and Cellular Biology.

[15]  G. Kreutzberg Microglia: a sensor for pathological events in the CNS , 1996, Trends in Neurosciences.