ATPase activity in isolated flagella from Euglena gracilis.

SYNOPSIS. The ATPase activity of isolated flagella was studied in Euglena gracilis strain Z in the presence of Mg++ or Ca++. With Mg++, the optimum activity was at pH 7 and with Ca++, at pH 9. The Km values were respectively 6.6 × 10−4 and 3.6 × 10−4. Activity was influenced also by temperature and ionic strength. Results with inhibitors of membrane ATPase suggest the presence of a specific contractile system in the flagella. Our results are compatible with a multicomponent enzymic system containing 2 active ATPases.

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