Endothelial cell growth on oxygen-containing films deposited by radio-frequency plasmas: the role of surface carbonyl groups.

Polystyrene substrates were modified by radio-frequency plasma deposition from mixtures of various organic vapors (acetone, methane, methanol, and formic acid) and oxygen. The resulting surfaces exhibited a wide range of surface oxygen concentrations, as measured by electron spectroscopy for chemical analysis (ESCA). The surface hydroxyl, carboxyl, and carbonyl groups were derivatized with trifluoroacetic anhydride, trifluoroethanol, or hydrazine, respectively, and their concentrations subsequently determined by ESCA. The growth of bovine aortic endothelial cells was found to increase with the surface carbonyl concentration but did not appear to correlate with the hydroxyl or carboxyl concentrations.

[1]  E. Jaffe,et al.  Synthesis and secretion of thrombospondin by cultured human endothelial cells , 1982, The Journal of cell biology.

[2]  P. Knox.  Kinetics of cell spreading in the presence of different concentrations of serum or fibronectin-depleted serum. , 1984, Journal of cell science.

[3]  E. Levine,et al.  Adult human endothelial cell compatibility with prosthetic graft material. , 1985, The Journal of surgical research.

[4]  Buddy D. Ratner,et al.  Plasma-deposited polymeric films prepared from carbonyl-containing volatile precursors: XPS chemical derivatization and static SIMS surface characterization , 1991 .

[5]  B D Ratner,et al.  Radiofrequency plasma deposition of oxygen-containing films on polystyrene and poly(ethylene terephthalate) substrates improves endothelial cell growth. , 1990, Journal of biomedical materials research.

[6]  B. Ratner,et al.  Synthesis, bulk characterization, and surface characterization of p-hydroxystyrene/styrene copolymers , 1988 .

[7]  D. W. Vinter,et al.  Expanded polytetrafluoroethylene vascular prostheses seeded with enzymatically derived and cultured canine endothelial cells. , 1982, Surgery.

[8]  G. Johnson,et al.  Cell responses to biomaterials. I: Adhesion and growth of vascular endothelial cells on poly(hydroxyethyl methacrylate) following surface modification by hydrolytic etching. , 1987, Journal of biomedical materials research.

[9]  C. R. Brundle,et al.  Electron Spectroscopy: Theory, Techniques and Applications , 1979 .

[10]  J. Feijen,et al.  The influence of protein adsorption on interactions of cultured human endothelial cells with polymers. , 1987, Journal of biomedical materials research.

[11]  A. Whittemore,et al.  Improved patency of collagen-impregnated grafts after in vitro autogenous endothelial cell seeding. , 1987, Journal of vascular surgery.

[12]  J. Forrester,et al.  Comparison of Systolic Blood Pressure Measurements by Auscultation and Visual Manometer Needle Jump , 2019, International journal of exercise science.

[13]  D. Gospodarowicz,et al.  Increased adherence of vascular endothelial cells to Biomer precoated with extracellular matrix. , 1981, Transactions - American Society for Artificial Internal Organs.

[14]  J. Forrester,et al.  Substrate hydroxylation and cell adhesion , 1986 .

[15]  R. W. Bussian,et al.  Short-term cell-attachment rates: a surface-sensitive test of cell-substrate compatibility. , 1987, Journal of biomedical materials research.

[16]  M. Reidy,et al.  Mechanisms of arterial graft healing. Rapid transmural capillary ingrowth provides a source of intimal endothelium and smooth muscle in porous PTFE prostheses. , 1986, The American journal of pathology.

[17]  E. Jaffe,et al.  Synthesis of antihemophilic factor antigen by cultured human endothelial cells. , 1973, The Journal of clinical investigation.

[18]  B. Zetter,et al.  Identification and isolation of endothelial cells based on their increased uptake of acetylated-low density lipoprotein , 1984, The Journal of cell biology.

[19]  N. Maroudas,et al.  Sulphonated polystyrene as an optimal substratum for the adhesion and spreading of mesenchymal cells in monovalent and divalent saline solutions , 1977, Journal of cellular physiology.

[20]  O. Weisz,et al.  Cell attachment and long-term growth on derivatizable polyacrylamide surfaces. , 1987, The Journal of biological chemistry.

[21]  G. Martin,et al.  Effects of cell adhesion of the substratum on the growth of chick embryo fibroblasts. , 1974, Experimental cell research.

[22]  J. H. Scofield,et al.  Hartree-Slater subshell photoionization cross-sections at 1254 and 1487 eV , 1976 .