Overcoming glucose repression in mixed sugar fermentation by co-expressing a cellobiose transporter and a β-glucosidase in Saccharomyces cerevisiae.

Glucose repression is one of the main limitations in mixed lignocellulosic sugar fermentation for cost-effective production of fuels and chemicals. Here we report a novel strategy to overcome glucose repression by co-expressing a cellobiose transporter and a β-glucosidase in an engineered d-xylose-utilizing Saccharomyces cerevisiae strain. The resulting strain can simultaneously utilize cellobiose and d-xylose for ethanol production.

[1]  C. Hollenberg,et al.  The molecular genetics of hexose transport in yeasts. , 1997, FEMS microbiology reviews.

[2]  J. Gancedo.  Yeast Carbon Catabolite Repression , 1998, Microbiology and Molecular Biology Reviews.

[3]  J. Nielsen,et al.  Investigation of the impact of MIG1 and MIG2 on the physiology of Saccharomyces cerevisiae. , 1999, Journal of biotechnology.

[4]  M. Carlson,et al.  Glucose repression in yeast. , 1999, Current opinion in microbiology.

[5]  M. D. Busto,et al.  Enzymatic Saccharification of Pretreated Wheat Straw by T. Reesei Cellulases and A. Niger β-Glucosidase , 2000 .

[6]  Filip Rolland,et al.  Glucose-sensing and -signalling mechanisms in yeast. , 2002, FEMS yeast research.

[7]  C. Roca,et al.  Engineering of carbon catabolite repression in recombinant xylose fermenting Saccharomyces cerevisiae , 2004, Applied Microbiology and Biotechnology.

[8]  G. Santangelo,et al.  Glucose Signaling in Saccharomyces cerevisiae , 2006, Microbiology and Molecular Biology Reviews.

[9]  Jack Saddler,et al.  Optimization of enzyme complexes for lignocellulose hydrolysis , 2007, Biotechnology and bioengineering.

[10]  David K. Johnson,et al.  Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production , 2007, Science.

[11]  B. Hahn-Hägerdal,et al.  Towards industrial pentose-fermenting yeast strains , 2007, Applied Microbiology and Biotechnology.

[12]  J. Pronk,et al.  Novel Evolutionary Engineering Approach for Accelerated Utilization of Glucose, Xylose, and Arabinose Mixtures by Engineered Saccharomyces cerevisiae Strains , 2008, Applied and Environmental Microbiology.

[13]  Huimin Zhao,et al.  Yeast Surface Display of Trifunctional Minicellulosomes for Simultaneous Saccharification and Fermentation of Cellulose to Ethanol , 2009, Applied and Environmental Microbiology.

[14]  B. Hahn-Hägerdal,et al.  Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway , 2009, Microbial cell factories.

[15]  Zengyi Shao,et al.  DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways , 2008, Nucleic acids research.

[16]  Akihiko Kondo,et al.  Alcoholic fermentation of xylose and mixed sugars using recombinant Saccharomyces cerevisiae engineered for xylose utilization , 2009, Applied Microbiology and Biotechnology.

[17]  Albert J. Vilella,et al.  Cellodextrin Transport in Yeast for Improved Biofuel Production , 2010, Science.

[18]  M. Sedlák,et al.  Establishment of l-arabinose fermentation in glucose/xylose co-fermenting recombinant Saccharomyces cerevisiae 424A(LNH-ST) by genetic engineering , 2010, Applied Microbiology and Biotechnology.