Hematology and Clinical Chemistry Values of Free-Ranging Basilisk Lizards (Basiliscus plumifrons) in Costa Rica

Abstract:  Twenty-three lizards were captured for this study, both males and females (12 males, 10 females, 1 undetermined), with a large range in body weights (40–286 g) appeared to be healthy based on activity level, physical examinations, and body condition scores. Heparinized blood samples from 20 free-ranging basilisk lizards (Basiliscus plumifrons) in Costa Rica were used for determining complete blood cell counts, plasma, and heparinized whole blood biochemical analysis. This information will serve as baseline reference data for future health assessment studies of free-ranging and captive basilisk lizards, as well as epidemiologic, conservation, and captive-breeding studies. A point-of-care analyzer was useful for this field study, and clinical chemistry values from heparinized whole blood samples were similar to values from plasma, which indicates that separation of plasma may not be necessary to process blood samples on site in remote areas. To the authors' knowledge, this is the first report of hematologic and plasma biochemical data from free-ranging B. plumifrons.

[1]  E. Greiner,et al.  Prevalence of Cryptosporidium Infection and Characteristics of Oocyst Shedding in a Breeding Colony of Leopard Geckos (Eublepharis macularius) , 2008, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[2]  C. H. Courtney,et al.  Relationship Between Separation Time of Plasma From Heparinized Whole Blood on Plasma Biochemical Analytes of Loggerhead Sea Turtles (Caretta caretta) , 2008, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[3]  F. Shofer,et al.  Assessment of a point-of-care biochemical analyzer and comparison with a commercial laboratory for the measurement of total protein and albumin concentrations in psittacines. , 2007, American journal of veterinary research.

[4]  J. Ramer,et al.  BIOCHEMICAL REFERENCE INTERVALS AND INTESTINAL MICROFLORA OF FREE-RANGING RICORD'S IGUANAS (CYCLURA RICORDII) , 2007, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[5]  C. Innis,et al.  Metabolic and respiratory status of cold-stunned Kemp’s ridley sea turtles (Lepidochelys kempii) , 2007, Journal of Comparative Physiology B.

[6]  C. Vaughan,et al.  Home range and habitat use of Basiliscus plumifrons (Squamata: Corytophanidae) in an active Costa Rican cacao farm , 2007 .

[7]  J. Iverson,et al.  Health Assessment of Allen Cays Rock Iguana, Cyclura cychlura inornata , 2006 .

[8]  D. DeNardo Chapter 23 – Reproductive Biology , 2006 .

[9]  G. Diethelm,et al.  Chapter 88 – Hematologic and Blood Chemistry Values in Reptiles , 2006 .

[10]  T. Campbell Clinical Pathology of Reptiles , 2006 .

[11]  R. Raskin,et al.  Temporal effects of 3 commonly used anticoagulants on hematologic and biochemical variables in blood samples from macaws and Burmese pythons. , 2005, Veterinary clinical pathology.

[12]  C. Innis,et al.  Characterizing the Hematologic and Plasma Chemistry Profiles of Captive Chinese Water Dragons, Physignathus cocincinus , 2005 .

[13]  K. Latimer,et al.  COMPARISON OF THE EFFECT OF DIPOTASSIUM ETHYLENEDIAMINETETRAACETIC ACID AND LITHIUM HEPARIN ON HEMATOLOGIC VALUES IN THE GREEN IGUANA (IGUANA IGUANA) , 2004, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[14]  E. Jacobson,et al.  Effects of blood processing techniques on sodium and potassium values: a comparison between Aldabra tortoises (Geochelone gigantea) and Burmese mountain tortoises (Manouria emys). , 2003, Veterinary clinical pathology.

[15]  P. Dennis,et al.  Plasma concentration of ionized calcium in healthy iguanas. , 2001, Journal of the American Veterinary Medical Association.

[16]  P. Dennis,et al.  Morphologic and cytochemical characteristics of blood cells and hematologic and plasma biochemical reference ranges in green iguanas. , 2001, Journal of the American Veterinary Medical Association.

[17]  K. Nagy,et al.  REFERENCE INTERVALS AND PHYSIOLOGIC ALTERATIONS IN HEMATOLOGIC AND BIOCHEMICAL VALUES OF FREE-RANGING DESERT TORTOISES IN THE MOJAVE DESERT , 1999, Journal of wildlife diseases.

[18]  S. Lavín,et al.  Effects of lithium heparin and tripotassium EDTA on hematologic values of Hermann's tortoises (Testudo hermanni). , 1998, Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians.

[19]  L. Guillette,et al.  The reproductive cycle of the female American alligator (Alligator mississippiensis). , 1997, General and comparative endocrinology.

[20]  Marla M. Ellman Hematology And Plasma Chemistry Of The Inland Bearded Dragon, Pogona vitticeps , 1997 .

[21]  A. Alberts,et al.  Seasonal Reproductive Cycle of the Desert Tortoise (Gopherus agassizii) in the Eastern Mojave Desert , 1994 .

[22]  G. Chambers,et al.  Slow Estradiol-induced Vitellogenesis in the Tuatara, Sphenodon punctatus , 1991, Physiological Zoology.

[23]  B. Rose Lizard Home Ranges: Methodology and Functions , 1982 .

[24]  R. Devender COMPARATIVE DEMOGRAPHY OF THE LIZARD BASILISCUS BASILISCUS , 1982 .

[25]  V. Lance Studies on the annual reproductive cycle of the female cobra, Naja naja. Seasonal variation in plasma inorganic ions. , 1976, Comparative biochemistry and physiology. A, Comparative physiology.

[26]  R. H. Moore,et al.  Some Grubbs-Type Statistics for the Detection of Several Outliers , 1972 .

[27]  W. J. Dixon,et al.  Analysis of Extreme Values , 1950 .