Islet-cell-surface antibodies in juvenile diabetes mellitus.

Using an indirect immunofluorescence test on suspensions of viable, insulin-producing islet cells from rats, we found that 32 per cent (28/88) of insulin-treated patients with juvenile diabetes have islet-cell-surface antibodies in their circulation. These antibodies also occurred in four of nine children with glucose intolerance, in one of 24 healthy children and in nondiabetic children with thyroid disorders. In the diabetic children, the immunofluorescent reaction was inhibited by preadsorption of serum to islet cells but was little affected by preadsorption to rat hepatocytes or erythrocytes or to acetone powders of various rat tissues, including pancreas. These results show that organ-specific, nonspecies-specific antibodies reactive with the cell surface of the islet cells can be present in serum from diabetic children, and provide an approach to investigation of immunopathological aspects of diabetes mellitus.

[1]  D. Silberberg,et al.  Antibodies to oligodendroglia in patients with multiple sclerosis. , 1977, The New England journal of medicine.

[2]  C. Betterle,et al.  Incidence and Significance of Islet-cell Autoantibodies in Different Types of Diabetes Mellitus , 1977, Diabetes.

[3]  W. Irvine,et al.  CLINICAL AND PATHOGENIC SIGNIFICANCE OF PANCREATIC-ISLET-CELL ANTIBODIES IN DIABETICS TREATED WITH ORAL HYPOGLYCÆMIC AGENTS , 1977, The Lancet.

[4]  A. Pestronk,et al.  Myasthenia gravis. Study of humoral immune mechanisms by passive transfer to mice. , 1977, The New England journal of medicine.

[5]  Å. Lernmark,et al.  Preparation and characterization of plasma membrane-enriched fractions from rat pancreatic islets , 1976, The Journal of cell biology.

[6]  J. Westman,et al.  Survival of isolated human islets of Langerhans maintained in tissue culture. , 1976, The Journal of clinical investigation.

[7]  C. Kahn,et al.  The syndromes of insulin resistance and acanthosis nigricans. Insulin-receptor disorders in man. , 1976, The New England journal of medicine.

[8]  D. Pyke,et al.  HISTOCOMPATIBILITY ANTIGENS IN DIABETIC IDENTICAL TWINS , 1975, The Lancet.

[9]  N. Maclaren,et al.  ANTIBODY TO CULTURED HUMAN INSULINOMA CELLS IN INSULIN-DEPENDENT DIABETES , 1975, The Lancet.

[10]  D. R. Gamble,et al.  ISLET-CELL ANTIBODIES IN JUVENILE DIABETES MELLITUS OF RECENT ONSET , 1975, The Lancet.

[11]  W. Dreyer,et al.  New immunolatex spheres: visual markers of antigens on lymphocytes for scanning electron microscopy , 1975, The Journal of cell biology.

[12]  Å. Lernmark,et al.  The use of dispersed pancreatic islet cells in measurements of transmembrane transport , 1975 .

[13]  E. Barnes,et al.  Antibodies to pancreatic islet cells in insulin-dependent diabetics with coexistent autoimmune disease. , 1974, Lancet.

[14]  D. Doniach,et al.  Islet-cell antibodies in diabetes mellitus with autoimmune polyendocrine deficiencies. , 1974, Lancet.

[15]  J. Nerup,et al.  HL-A antigens and diabetes mellitus. , 1974, Lancet.

[16]  B. Smith,et al.  THYROID-STIMULATING IMMUNOGLOBULINS IN GRAVES' DISEASE , 1974 .

[17]  J. Bourke,et al.  The thyrotrophin receptor in guinea-pig thyroid homogenate: interaction with the long-acting thyroid stimulator. , 1974, The Journal of endocrinology.

[18]  K. Meyer zum Büschenfelde,et al.  Liver-specific antigens of different species. II. Localization of a membrane antigen at cell surface of isolated hepatocytes. , 1974, Clinical and experimental immunology.

[19]  J. Nerup,et al.  ANTI‐PANCREATIC, CELLULAR HYPERSENSITIVITY IN DIABETES MELLITUS. ANTIGENIC ACTIVITY OF FETAL CALF PANCREAS AND CORRELATION WITH CLINICAL TYPE OF DIABETES , 1973, Acta allergologica.

[20]  J. Patrick,et al.  Autoimmune Response to Acetylcholine Receptor , 1973, Science.

[21]  J. Nerup,et al.  Antipancreatic Cellular Hypersensitivity in Diabetes Mellitus , 1971, Diabetes.

[22]  B. Clarke,et al.  Thyroid and gastric autoimmunity in patients with diabetes mellitus. , 1970, Lancet.

[23]  W. Gepts.  Pathologic Anatomy of the Pancreas in Juvenile Diabetes Mellitus , 1965, Diabetes.

[24]  G. Zampa,et al.  HUMAN INSULIN ANTIBODIES DETECTED BY IMMUNOFLUORESCENT TECHNIQUE , 1964 .

[25]  G. Möller.  DEMONSTRATION OF MOUSE ISOANTIGENS AT THE CELLULAR LEVEL BY THE FLUORESCENT ANTIBODY TECHNIQUE , 1961, The Journal of experimental medicine.

[26]  B. Hellman.  The total volume of the pancreatic islet tissue at different ages of the rat. , 2009, Acta pathologica et microbiologica Scandinavica.