Amphotericin B Supplementation of Cold Storage Media to Treat Fungal Contamination of Donor Cornea Transplant Tissue.

Methods: 80 human donor corneas were placed in individual vials of Optisol GS (Bausch and Lomb, US). Candida albicans was added to the treated groups (N=20). The corneas remained in the storage media for 3 days, which was followed by a 2-hour warming period (to simulate the tissue processing for DSAEK or DMEK). This was followed by an additional 1 day of storage. A fungal assay was then performed. The groups: Group 1) The control group and had no amphotericin b 0.225 ug/ml added to the media. Group 2) Amphotericin b 0.225 ug/ml was added to the storage media when the donor cornea was initially placed in the media. Group 3) Amphotericin b 0.225 ug/ml was added to the storage media after the simulated warming period (day 3). Group 4) Amphotericin b 0.225 ug/ml was added at the initial time point and after the simulated warming period.

[1]  M. Macsai,et al.  Incidence and Outcomes of Positive Donor Rim Cultures and Infections in the Cornea Preservation Time Study , 2018, Cornea.

[2]  E. Espana,et al.  Candida Endophthalmitis After Descemet Stripping Automated Endothelial Keratoplasty With Grafts From Both Eyes of a Donor With Possible Systemic Candidiasis , 2018, Cornea.

[3]  E. Tu,et al.  Association Between Fungal Contamination and Eye Bank–Prepared Endothelial Keratoplasty Tissue: Temperature-Dependent Risk Factors and Antifungal Supplementation of Optisol–Gentamicin and Streptomycin , 2017, JAMA ophthalmology.

[4]  Gregory A. Schmidt,et al.  Incidence and Outcomes of Positive Donor Corneoscleral Rim Fungal Cultures after Keratoplasty. , 2017, Ophthalmology.

[5]  Christopher G. Stoeger,et al.  Report of the Eye Bank Association of America Medical Review Subcommittee on Adverse Reactions Reported From 2007 to 2014 , 2016, Cornea.

[6]  M. Zegans,et al.  Candida Interface Infections After Descemet Stripping Automated Endothelial Keratoplasty , 2016, Cornea.

[7]  B. Jeng,et al.  The Effect of Light Exposure on the Efficacy and Safety of Amphotericin B in Corneal Storage Media. , 2016, JAMA ophthalmology.

[8]  E. Tu,et al.  Intrastromal Antifungal Injection With Secondary Lamellar Interface Infusion for Late-Onset Infectious Keratitis After DSAEK , 2014, Cornea.

[9]  Y. Nahum,et al.  Interface Infection After Descemet Stripping Automated Endothelial Keratoplasty: Outcomes of Therapeutic Keratoplasty , 2014, Cornea.

[10]  J. Keenan,et al.  Efficacy and safety of antifungal additives in Optisol-GS corneal storage medium. , 2014, JAMA ophthalmology.

[11]  A. Villarrubia,et al.  Candida Keratitis after Descemet Stripping with Automated Endothelial Keratoplasty , 2014, European journal of ophthalmology.

[12]  F. Yu,et al.  Report of the Eye Bank Association of America Medical Advisory Board Subcommittee on Fungal Infection After Corneal Transplantation , 2013, Cornea.

[13]  Gregory A. Schmidt,et al.  Impact of Eye Bank Lamellar Tissue Cutting for Endothelial Keratoplasty on Bacterial and Fungal Corneoscleral Donor Rim Cultures After Corneal Transplantation , 2012, Cornea.

[14]  R. A. Cramer,et al.  Hypoxia and Fungal Pathogenesis: To Air or Not To Air? , 2012, Eukaryotic Cell.

[15]  C. Villa-Collar,et al.  Late-onset Candida Keratitis after Descemet Stripping Automated Endothelial Keratoplasty: Clinical and Confocal Microscopic Report , 2011, European journal of ophthalmology.

[16]  Yoichi Tanaka,et al.  Severe donor-related Candida keratitis after Descemet’s stripping automated endothelial keratoplasty , 2011, Graefe's Archive for Clinical and Experimental Ophthalmology.

[17]  H. Grossniklaus,et al.  Interface fungal keratitis after endothelial keratoplasty: a clinicopathological report. , 2011, Ophthalmic surgery, lasers & imaging : the official journal of the International Society for Imaging in the Eye.

[18]  J. Mehta,et al.  Fungal Endophthalmitis After Descemet Stripping Automated Endothelial Keratoplasty-A Case Report , 2010, Cornea.

[19]  K. Goins,et al.  Donor-Related Candida Keratitis After Descemet Stripping Automated Endothelial Keratoplasty , 2009, Cornea.

[20]  R. Fish,et al.  Candida Keratitis After Descemet Stripping and Automated Endothelial Keratoplasty , 2009, Cornea.

[21]  D. Ritterband,et al.  The incidence of fungal keratitis and endophthalmitis following penetrating keratoplasty. , 2005, Cornea.

[22]  M. Durand,et al.  Candidal Endophthalmitis After Keratoplasty , 2001, Cornea.

[23]  M. Bohnke,et al.  Incidence of Bacterial and Fungal Contamination of Donor Corneas Preserved by Organ Culture , 1995, Cornea.

[24]  M. Trousdale,et al.  Efficacy and Safety of Gentamicin and Streptomycin in Optisol‐GS, a Preservation Medium for Donor Corneas , 1995, Cornea.

[25]  J. Nelson,et al.  Fungal contamination in organ culture. , 1983, Archives of ophthalmology.

[26]  A. S. Wiener,et al.  Transplantation , 1963 .