Respiratory oscillations in yeast: clock‐driven mitochondrial cycles of energization

Respiratory oscillations in continuous yeast cultures can be accounted for by cyclic energization of mitochondria, dictated by the demands of a temperature‐compensated ultradian clock with a period of 50 min. Inner mitochondrial membranes show both ultrastructural modifications and electrochemical potential changes. Electron transport components (NADH and cytochromes c and c oxidase) show redox state changes as the organisms cycle between their energized and de‐energized phases. These regular cycles are transiently perturbed by uncouplers of energy conservation, with amplitudes more affected than period; that the characteristic period is restored after only one prolonged cycle, indicates that mitochondrial energy generation is not part of the clock mechanism itself, but is responding to energetic requirement.

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