Electrochemical impedance monitoring of immunochemical reactions using varying IDE geometries for signal enhancement

Electrochemical Impedance Spectroscopy (EIS) has been applied to the detection of analytes for immunosensors [1-3]. The development of hand held devices based on this technique is a very promising prospect for point-of-care applications and is an attractive alternative to laboratory-based immunochemical analysis [1, 4]. The work in this paper will focus primarily on the development of an EIS method of transduction for immunoassay detection that could be potentially introduced into a hand held point-of-care device. Varying geometries of IDEs will be reported and discussed to improve the detection of antigen.

[1]  M. A. Alonso-Lomillo,et al.  Recent developments in the field of screen-printed electrodes and their related applications. , 2007, Talanta.

[2]  Andrzej Wieckowski,et al.  Interfacial Electrochemistry: Theory: Experiment, and Applications , 1999 .

[3]  Bobby Pejcic,et al.  Impedance spectroscopy: Over 35 years of electrochemical sensor optimization , 2006 .

[4]  J. Samitier,et al.  Low cost micro-Coulter counter with hydrodynamic focusing , 2007 .

[5]  L. Hermans,et al.  Nanoscaled interdigitated titanium electrodes for impedimetric biosensing , 2000 .

[6]  A Warsinke,et al.  Electrochemical immunoassays , 2000, Fresenius' journal of analytical chemistry.

[7]  Willy Sansen,et al.  Nanoscaled interdigitated electrode arrays for biochemical sensors , 1998 .

[8]  Robert Koncki,et al.  Recent developments in potentiometric biosensors for biomedical analysis. , 2007, Analytica chimica acta.

[9]  T. Osaka,et al.  High efficiency electrochemical immuno sensors using 3D comb electrodes. , 2005, Biosensors & bioelectronics.

[10]  Olivier Lazcka,et al.  Pathogen detection: a perspective of traditional methods and biosensors. , 2007, Biosensors & bioelectronics.

[11]  Liviu Moraru,et al.  Catheter-based impedance measurements in the right atrium for continuously monitoring hematocrit and estimating blood viscosity changes; an in vivo feasibility study in swine. , 2004, Biosensors & bioelectronics.

[12]  Ma Jie,et al.  An electrochemical impedance immunoanalytical method for detecting immunological interaction of human mammary tumor associated glycoprotein and its monoclonal antibody , 1999 .

[13]  C. Ahn,et al.  Functionalized nano interdigitated electrodes arrays on polymer with integrated microfluidics for direct bio-affinity sensing using impedimetric measurement , 2007 .

[14]  Jonathan M. Cooper,et al.  Biosensors : a practical approach , 2004 .

[15]  Eliora Z Ron,et al.  Biosensing environmental pollution. , 2007, Current opinion in biotechnology.

[16]  Dorielle T. Price,et al.  Effect of electrode geometry on the impedance evaluation of tissue and cell culture , 2007 .

[17]  Jian-hui Jiang,et al.  An electrochemical impedance immunosensor with signal amplification based on Au-colloid labeled antibody complex , 2006 .

[18]  R. Pei,et al.  Layer-by-layer assembly of multilayer films composed of avidin and biotin-labeled antibody for immunosensing. , 2003, Biosensors & bioelectronics.

[19]  M. D. Rooij,et al.  Electrochemical Methods: Fundamentals and Applications , 2003 .