A histochemical study of fibronectin (FN) of the large intestine by the immunoperoxidase technique.

Histochemical studies of fibronectin in cancer and adenoma of the large intestine were conducted by the immunoperoxidase technique. The fibronectin immunohistochemical reaction was present in frozen sections, whereas it was obviously suppressed in the formalin-fixed sections. In the normal mucosa of the large intestine, the reaction was strong in the basement membrane of epithelial cells and weaker around the interstitial cells of the lamina propria mucosa. The reaction in cancer tissues was negative in the basal part of the gland, while the interstitial connective tissue was strongly positive for fibronectin. In adenoma, there was a correlation between the intensity of the fibronectin reaction and the degree of epithelial cell atypism; that is, in a gland with severe atypism the reaction was very weak or absent, while in a gland with mild atypism a weak reaction still persisted. It is concluded that this technique will serve as a useful supplementary technique for the purpose of studying the biological role of fibronectin, and that the fibronectin reaction has a close relationship with malignancy and with cellular atypism of the epithelium of the large intestine.

[1]  A. Vaheri,et al.  Fibronectin in human solid tumors , 1981, International journal of cancer.

[2]  P. Birembaut,et al.  Loss of fibronectin in human breast cancer. , 1980, Cell biology international reports.

[3]  E. Engvall,et al.  Butyrate restores fibronectin at cell surface of transformed cells. , 1980, Experimental cell research.

[4]  T. Puck,et al.  Deposition of fibronectin in the course of reverse transformation of Chinese hamster ovary cells by cyclic AMP. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Foidart,et al.  Dexamethasone-induced accumulation of a fibronectin and collagen extracellular matrix in transformed human cells , 1979, Nature.

[6]  Kenneth M. Yamada,et al.  Fibronectins—adhesive glycoproteins of cell surface and blood , 1978, Nature.

[7]  R. Hynes,et al.  Restoration of normal morphology, adhesion and cytoskeleton in transformed cells by addition of a transformation-sensitive surface protein , 1977, Cell.

[8]  E. Engvall,et al.  Immunological interspecies cross-reactions of fibroblast surface antigen (fibronectin). , 1976, Immunochemistry.

[9]  A. Vaheri,et al.  Changes in expression of fibroblast surface antigen (SFA) during cytodifferentiation and heterokaryon formation. , 1976, Differentiation; research in biological diversity.

[10]  I. Pastan,et al.  Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[11]  E. Ruoslahti,et al.  A common cell-type specific surface antigen in cultured human glial cells and fibroblasts: loss in malignant cells , 1976, The Journal of experimental medicine.

[12]  E. Ruoslahti,et al.  Interaction of soluble fibroblast surface antigen with fribrinogen and fibrin , 1975, The Journal of experimental medicine.

[13]  J. Bye,et al.  Density and cell cycle dependence of cell surface proteins in hamster fibroblasts. , 1974, Cell.

[14]  K. Yamada,et al.  Isolation of a major cell surface glycoprotein from fibroblasts. , 1974, Proceedings of the National Academy of Sciences of the United States of America.

[15]  E. Ruoslahti,et al.  Novel human serum protein from fibroblast plasma membrane , 1974, Nature.

[16]  R. Hynes.  Alteration of cell-surface proteins by viral transformation and by proteolysis. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[17]  E. Ruoslahti,et al.  Fibroblast surface antigen: a new serum protein. , 1973, Biochimica et biophysica acta.

[18]  M. Mosesson,et al.  The cold-insoluble globulin of human plasma. I. Purification, primary characterization, and relationship to fibrinogen and other cold-insoluble fraction components. , 1970, The Journal of biological chemistry.

[19]  Morson Bc.  Some peculiarities in the histology of intestinal polyps , 1962 .