Thiol/disulfide homeostasis impaired in patients with primary Sjögren's syndrome

Background Primary Sjögren's syndrome (pSS) is a disease associated with the overexpression of proinflammatory cytokines, and oxidative stress is one of the factors responsible for its etiopathogenesis. This study aimed to investigate the thiol/disulphide homeostasis in pSS patients. Methods The study included 68 pSS patients and 69 healthy controls. Thiol/disulphide homeostasis (total thiol, native thiol, and disulphide levels) was measured using the automatic spectrophotometric method developed by Erel and Neselioglu, and the results of the 2 groups were compared. Results The gender and age distributions of the pSS and control groups were similar (P = 0.988 and P = 0.065). Total thiol and native thiol levels were lower in the pSS group than in the control group (470.08 ± 33.65 µmol/L vs. 528.21 ± 44.99 µmol/L, P < 0.001, and 439.14 ± 30.67 µmol/L vs. 497.56 ± 46.70 µmol/L, P < 0.001, respectively). There were no differences in disulphide levels between groups [17.00 (range 0.70-217.0) µmol/L vs. 14.95 (range 2.10-40.10) µmol/L, P = 0.195]. Conclusions It was concluded that the thiol/disulphide balance shifted towards disulphide in patients with pSS.

[1]  A. Kirmit,et al.  Thiol/disulphide balance in patients with psoriasis , 2020, Postepy dermatologii i alergologii.

[2]  S. Neşelioğlu,et al.  Thiol/disulfide homeostasis and ischemia modified albumin levels in autoimmune gastritis and their relations with gastric emptying , 2019, Turkish journal of medical sciences.

[3]  O. Erel,et al.  Dynamic thiol and disulphide homoeostasis in fibromyalgia , 2019, Archives of medical science : AMS.

[4]  H. Çelik,et al.  An Investigation of Oxidative Stress and Thiol/Disulphide Homeostasis in Graves’ Disease , 2019, Medicina.

[5]  G. Dogan,et al.  Evaluation of Erythroid Disturbance and Thiol-Disulphide Homeostasis in Patients with Psoriasis , 2018, BioMed research international.

[6]  O. Erel,et al.  Can the Thiol/Disulfide Imbalance Be a Predictor of Colchicine Resistance in Familial Mediterranean Fever? , 2017, Journal of Korean medical science.

[7]  O. Erel,et al.  Thiol/disulphide homeostasis in celiac disease , 2017, World journal of gastrointestinal pharmacology and therapeutics.

[8]  L. Criswell,et al.  2016 American College of Rheumatology/European League Against Rheumatism Classification Criteria for Primary Sjögren's Syndrome: A Consensus and Data‐Driven Methodology Involving Three International Patient Cohorts , 2017, Arthritis & rheumatology.

[9]  O. Erel,et al.  Thiol/disulphide homeostasis in pregnant women with Familial Mediterranean fever , 2016, Redox report : communications in free radical research.

[10]  Ö. Erel,et al.  The relation between oxidative stress parameters, ischemic stroke, and hemorrhagic stroke. , 2015, Turkish journal of medical sciences.

[11]  O. Erel,et al.  A novel and automated assay for thiol/disulphide homeostasis. , 2014, Clinical biochemistry.

[12]  Rafael Radi,et al.  The thiol pool in human plasma: the central contribution of albumin to redox processes. , 2013, Free radical biology & medicine.

[13]  U. Jakob,et al.  Oxidant Sensing by Reversible Disulfide Bond Formation* , 2013, The Journal of Biological Chemistry.

[14]  R. Omdal,et al.  Oxidative stress, as measured by protein oxidation, is increased in primary Sjøgren's syndrome , 2012, Free radical research.

[15]  J. Bijlsma,et al.  Increased expression of interleukin-7 in labial salivary glands of patients with primary Sjögren's syndrome correlates with increased inflammation. , 2010, Arthritis and rheumatism.

[16]  J. Čejková,et al.  Decreased expression of antioxidant enzymes in the conjunctival epithelium of dry eye (Sjögren's syndrome) and its possible contribution to the development of ocular surface oxidative injuries. , 2008, Histology and histopathology.

[17]  Seiji Kawano,et al.  Thioredoxin may exert a protective effect against tissue damage caused by oxidative stress in salivary glands of patients with Sjögren's syndrome. , 2007, The Journal of rheumatology.

[18]  K. Mishima,et al.  Possible Involvement of Oxidative Stress in Salivary Gland of Patients with Sjögren’s Syndrome , 2007, Pathobiology.

[19]  M. Ramos-Casals,et al.  Primary Sjögren ’ s syndrome : current and emergent aetiopathogenic concepts , 2005 .

[20]  A. Tzioufas,et al.  Primary Sjögren’s syndrome: new clinical and therapeutic concepts , 2004, Annals of the rheumatic diseases.

[21]  Dean P. Jones,et al.  Glutathione and thioredoxin redox during differentiation in human colon epithelial (Caco-2) cells. , 2002, American journal of physiology. Gastrointestinal and liver physiology.

[22]  Hannu Uusitalo,et al.  Clinical follow up study of 87 patients with sicca symptoms (dryness of eyes or mouth, or both) , 1999, Annals of the rheumatic diseases.

[23]  J. Oppenheimer,et al.  Ontogenesis of 3,5,3'-triiodothyronine receptors in neonatal rat brain: dissociation between receptor concentration and stimulation of oxygen consumption by 3,5,3'-triiodothyronine. , 1978, Endocrinology.

[24]  D. Chisholm,et al.  Labial salivary gland biopsy in Sjögren's disease , 1968, Journal of clinical pathology.

[25]  L. Klassen,et al.  Relevance of the Antioxidant Properties of Methotrexate and Doxycycline to their Treatment of Cardiovascular Disease. , 2019, Pharmacology & therapeutics.