Anaerobic treatment of cassava stillage for hydrogen and methane production in continuously stirred

Abstract Anaerobic hydrogen and methane production from cassava stillage in continuously stirred tank reactor (CSTR) were investigated in this study. Results showed that the heat-pretreatment of inoculum did not enhance hydrogen yield compared to raw inoculum under mesophilic condition after continuous operation. However, the hydrogen yield increased from about 14 ml H 2 /gVS under mesophilic condition to 69.6 ml H 2 /gVS under thermophilic condition due to the decrease of propionate concentration and inhibition of homoacetogens. Therefore, temperature was demonstrated to be more important than pretreatment of inoculum to enhance the hydrogen production. Under high organic loading rate (OLR) (>10 gVS/(L·d)), the two-phase thermophilic CSTR for hydrogen and methane production was stable with hydrogen and methane yields of 56.6 mlH 2 /gVS and 249 mlCH 4 /gVS. The one-phase thermophilic CSTR for methane production failed due to the accumulation of both acetate and propionate, leading to the pH lower than 6. Instead of propionate alone, the accumulations of both acetate and propionate were found to be related to the breakdown of methane reactor.

[1]  Qi Zhou,et al.  Evaluation of pretreatment methods on mixed inoculum for both batch and continuous thermophilic biohydrogen production from cassava stillage. , 2010, Bioresource technology.

[2]  Hang-Sik Shin,et al.  Continuous biohydrogen production in a CSTR using starch as a substrate , 2008 .

[3]  Yu-You Li,et al.  A pH- and temperature-phased two-stage process for hydrogen and methane production from food waste , 2008 .

[4]  G Lettinga,et al.  Thermophilic anaerobic digestion of high strength wastewaters , 1985, Biotechnology and bioengineering.

[5]  G. Luo,et al.  Exploring optimal conditions for thermophilic fermentative hydrogen production from cassava stillage , 2010 .

[6]  L. Evison,et al.  Effect of propionate toxicity on methanogen-enriched sludge, Methanobrevibacter smithii, and Methanospirillum hungatii at different pH values , 1991, Applied and environmental microbiology.

[7]  Hanqing Yu,et al.  Effects of temperature and substrate concentration on biological hydrogen production from starch , 2009 .

[8]  Qi Zhou,et al.  Enhanced treatment efficiency of an anaerobic sequencing batch reactor (ASBR) for cassava stillage with high solids content. , 2009, Journal of bioscience and bioengineering.

[9]  Michael Kornaros,et al.  Using cheese whey for hydrogen and methane generation in a two-stage continuous process with alternative pH controlling approaches. , 2009, Bioresource technology.

[10]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[11]  Jianlong Wang,et al.  FACTORS INFLUENCING FERMENTATIVE HYDROGEN PRODUCTION: A REVIEW , 2009 .

[12]  Jo-Shu Chang,et al.  Exploring optimal environmental factors for fermentative hydrogen production from starch using mixed anaerobic microflora , 2008 .

[13]  Heguang Zhu,et al.  Evaluation of alternative methods of preparing hydrogen producing seeds from digested wastewater sludge , 2006 .

[14]  Sangeun Oh,et al.  The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. , 2003, Environmental science & technology.

[15]  Irini Angelidaki,et al.  Hydrogen and methane production from household solid waste in the two-stage fermentation process. , 2006, Water research.

[16]  Jianlong Wang,et al.  Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge , 2008 .

[17]  Kefa Cen,et al.  Cogeneration of hydrogen and methane from glucose to improve energy conversion efficiency , 2008 .

[18]  Peter Seto,et al.  Co-production of hydrogen and methane from potato waste using a two-stage anaerobic digestion process. , 2008, Bioresource technology.

[19]  Hang-Sik Shin,et al.  Optimization of continuous hydrogen fermentation of food waste as a function of solids retention time independent of hydraulic retention time , 2008 .

[20]  K. Haga,et al.  Effect of fermentation temperature on hydrogen production from cow waste slurry by using anaerobic microflora within the slurry , 2007, Applied Microbiology and Biotechnology.