Towards detection of unknown GMOs

The state-of-the-art technology for detection of genetically modified organisms (GMOs) is polymerase chain reaction. The targets are novel sequences such as genes, but the insertion locus sequences are also important for GMO identification. GMOs go through a series of developmental stages, in which field trials precede commercial releases. GMO developers only exceptionally publish details on inserted novel sequences unless such publication is, for example, required by legislation. Information on novel sequences of GMOs may therefore be sparse, and in some cases completely missing, making the development and implementation of detection methods extremely difficult. In this chapter, we present an overview of the problems and proposed possible solutions to challenges associated with detection and correct identification of GMOs, with particular emphasis on unknown GMOs

[1]  T. Tengs,et al.  Non-prejudiced Detection and Characterization of Genetic Modifications , 2010 .

[2]  L. Partis,et al.  Detection and identification of multiple genetically modified events using DNA insert fingerprinting , 2010, Analytical and bioanalytical chemistry.

[3]  Nada Lavrač,et al.  Knowledge-technology-based discovery of unauthorized genetically modified organisms , 2010, Analytical and bioanalytical chemistry.

[4]  T. Ruttink,et al.  Molecular toolbox for the identification of unknown genetically modified organisms , 2010, Analytical and bioanalytical chemistry.

[5]  Hans-Ulrich Waiblinger,et al.  A practical approach to screen for authorised and unauthorised genetically modified plants , 2010, Analytical and bioanalytical chemistry.

[6]  Hermann Broll,et al.  New approaches in GMO detection , 2010, Analytical and bioanalytical chemistry.

[7]  N. Roosens,et al.  A theoretical introduction to “Combinatory SYBR®Green qPCR Screening”, a matrix-based approach for the detection of materials derived from genetically modified plants , 2009, Analytical and bioanalytical chemistry.

[8]  Nada Lavrac,et al.  GMOtrack: generator of cost-effective GMO testing strategies. , 2009, Journal of AOAC International.

[9]  T. Tengs,et al.  Characterization of unknown genetic modifications using high throughput sequencing and computational subtraction , 2009, BMC biotechnology.

[10]  A. Holck,et al.  Quantitative, multiplex ligation-dependent probe amplification for the determination of eight genetically modified maize events , 2009 .

[11]  Hermann Broll,et al.  Real-Time PCR-Based Ready-to-Use Multi-Target Analytical System for GMO Detection , 2009 .

[12]  M. Pla,et al.  Multiplex polymerase chain reaction-capillary gel electrophoresis: a promising tool for GMO screening--assay for simultaneous detection of five genetically modified cotton events and species. , 2009, Journal of AOAC International.

[13]  Myriam Sneyers,et al.  A PCR-microarray method for the screening of genetically modified organisms , 2009 .

[14]  J. Mano,et al.  Real-time PCR array as a universal platform for the detection of genetically modified crops and its application in identifying unapproved genetically modified crops in Japan. , 2009, Journal of agricultural and food chemistry.

[15]  Marleen M. Voorhuijzen,et al.  Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction , 2008, BMC Genomics.

[16]  V. Laval,et al.  A high-throughput multiplex method adapted for GMO detection. , 2008, Journal of agricultural and food chemistry.

[17]  Yves Bertheau,et al.  Gene stacking in transgenic plants: towards compliance between definitions, terminology, and detection within the EU regulatory framework. , 2008, Environmental biosafety research.

[18]  K. Gruden,et al.  NAIMA: target amplification strategy allowing quantitative on-chip detection of GMOs , 2008, Nucleic acids research.

[19]  A. Holst-Jensen.  GMO testing—trade, labeling or safety first? , 2008, Nature Biotechnology.

[20]  Yves Bertheau,et al.  Validation of the performance of a GMO multiplex screening assay based on microarray detection , 2008 .

[21]  K. Gruden,et al.  Detection of nonauthorized genetically modified organisms using differential quantitative polymerase chain reaction: application to 35S in maize. , 2008, Analytical biochemistry.

[22]  A. Holck,et al.  Determination of eight genetically modified maize events by quantitative, multiplex PCR and fluorescence capillary gel electrophoresis , 2008 .

[23]  M. Querci,et al.  Innovative Application of Fluorescent Microsphere Based Assay for Multiple GMO Detection , 2008 .

[24]  Arne Holst-Jensen,et al.  Microarray-based method for detection of unknown genetic modifications , 2007, BMC biotechnology.

[25]  V. Laval,et al.  A strategy for designing multi-taxa specific reference gene systems. example of application--ppi phosphofructokinase (ppi-PPF) used for the detection and quantification of three taxa: maize (Zea mays), cotton (Gossypium hirsutum) and rice (Oryza sativa). , 2007, Journal of agricultural and food chemistry.

[26]  Y. Devos,et al.  Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples , 2007 .

[27]  José Luis La Paz,et al.  A new PCR‐CGE (size and color) method for simultaneous detection of genetically modified maize events , 2006, Electrophoresis.

[28]  Patrick Philipp,et al.  A Microarray-based Detection System for Genetically Modified (GM) Food Ingredients , 2006, Plant Molecular Biology.

[29]  K. Rudi,et al.  Differentiation of important and closely related cereal plant species (Poaceae) in food by hybridization to an oligonucleotide array. , 2005, Journal of agricultural and food chemistry.

[30]  Heng Zhao,et al.  Rapid and reliable detection and identification of GM events using multiplex PCR coupled with oligonucleotide microarray. , 2005, Journal of agricultural and food chemistry.

[31]  Arne Holst-Jensen,et al.  Design of a DNA chip for detection of unknown genetically modified organisms (GMOs) , 2005, Bioinform..

[32]  Arne Holst-Jensen,et al.  PCR technology for screening and quantification of genetically modified organisms (GMOs) , 2003, Analytical and bioanalytical chemistry.

[33]  J. Stave.  Protein immunoassay methods for detection of biotech crops: applications, limitations, and practical considerations. , 2002, Journal of AOAC International.

[34]  K. G. Berdal,et al.  Roundup Ready® soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses , 2001 .

[35]  A. Holck,et al.  Detection of eight GMO maize events by qualitative, multiplex PCR and fluorescence capillary gel electrophoresis , 2008 .

[36]  J. Fox.  EPA re-evaluates StarLink license , 2001, Nature Biotechnology.