Quantitative trait loci underlying the development of seed composition in soybean (Glycine max L. Merr.).

One hundred and forty-three F2:7 recombinant inbred lines (RILs) developed from the cross of soybean cultivars 'Charleston' and 'Dongnong 594' were analyzed for the quantitative trait loci (QTLs) underlying protein or oil content at 6 different developmental stages by composite interval mapping with a mixed genetic model. The genotype x environment (GxE) interactions of the QTLs were also evaluated. Nineteen (2004) and 33 (2005) unconditional QTLs underlying seed protein or oil content at the different developmental stages were mapped onto 8 and 9 linkage groups, respectively. The proportion of phenotypic variation explained by these QTLs ranged from 6.26% to 30.52% and from 5.38% to 28.47%, respectively. Fourteen (2004) and 21 (2005) conditional QTLs underlying seed protein or oil content were mapped onto 5 and 8 linkage groups, respectively. The proportion of phenotypic variation explained by these QTLs ranged from 2.97% to 29.68% and from 5.42% to 31.96%, respectively. The numbers and types of QTLs and the genetic effect for the two traits were different at each developmental stage. However, several genomic regions that simultaneously control the development of both traits were detected. The genetic effect on protein content and oil content was opposite for loci in the marker interval Satt335-SSatt334, reflecting a negative correlation of protein content and oil content. A G x E interaction effect of some QTLs underlying protein or oil content at different growth periods was observed.

[1]  J. Vollmann,et al.  Seed quality QTLs identified in a molecular map of early maturing soybean , 2001, Theoretical and Applied Genetics.

[2]  Jun Zhu,et al.  Genetic analysis for developmental behavior of some seed quality traits in Upland cotton (Gossypum hirsutum L.) , 2004, Euphytica.

[3]  Jun Zhu,et al.  Molecular dissection of developmental behavior of plant height in rice (Oryza sativa L.). , 1998, Genetics.

[4]  J Li,et al.  Dominance is the major genetic basis of heterosis in rice as revealed by QTL analysis using molecular markers. , 1995, Genetics.

[5]  Perry B. Cregan,et al.  Genetic Mapping of Agronomic Traits Using Recombinant Inbred Lines of Soybean , 1996 .

[6]  D. Hyten,et al.  Seed quality QTL in a prominent soybean population , 2004, Theoretical and Applied Genetics.

[7]  D. Sleper,et al.  RFLP markers associated with soybean cyst nematode resistance and seed composition in a ‘Peking’בEssex’ population , 1999, Theoretical and Applied Genetics.

[8]  K. Lark,et al.  Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.) , 1993, Theoretical and Applied Genetics.

[9]  D. Ashley,et al.  RFLP loci associated with soybean seed protein and oil content across populations and locations , 1996, Theoretical and Applied Genetics.

[10]  Wenbin Li,et al.  Impact of epistasis and QTL x environment interaction on the accumulation of seed mass of soybean (Glycine max L. Merr.). , 2008, Genetics research.

[11]  Yunbi Xu,et al.  Quantitative trait loci: separating, pyramiding, and cloning , 1997 .

[12]  Genlou Sun,et al.  Quantitative trait loci analysis for the developmental behavior of Soybean (Glycinemax L. Merr.) , 2006, Theoretical and Applied Genetics.

[13]  R. Shoemaker,et al.  Mapping QTL for Seed Protein and Oil Content in Eight Soybean Populations , 1997 .

[14]  W. Fehr,et al.  Incorporation of High-Protein, Exotic Germplasm into Soybean Populations by 2- and 3-way Crosses 1 , 1970 .

[15]  James E. Specht,et al.  The Seed Protein, Oil, and Yield QTL on Soybean Linkage Group I , 2003 .

[16]  Jun Zhu,et al.  Analysis of conditional genetic effects and variance components in developmental genetics. , 1995, Genetics.

[17]  P. Gresshoff,et al.  Quantitative trait loci for agronomic and seed quality traits in an F2 and F4:6 soybean population , 2003, Euphytica.

[18]  J. Jones,et al.  Kjeldahl method for nitrogen determination. , 1991 .

[19]  Z B Zeng,et al.  Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[20]  F. Thibaud-Nissen,et al.  Microarrays for global expression constructed with a low redundancy set of 27,500 sequenced cDNAs representing an array of developmental stages and physiological conditions of the soybean plant , 2004, BMC Genomics.

[21]  Richard D. Thompson,et al.  Reserve accumulation in legume seeds. , 2008, Comptes rendus biologies.

[22]  J. E. Specht,et al.  A new integrated genetic linkage map of the soybean , 2004, Theoretical and Applied Genetics.

[23]  B. S. Weir,et al.  Diallel analysis for sex-linked and maternal effects , 2004, Theoretical and Applied Genetics.

[24]  A. Worland,et al.  Time-related mapping of quantitative trait loci underlying tiller number in rice. , 1999, Genetics.

[25]  Z. Zeng.  Precision mapping of quantitative trait loci. , 1994, Genetics.

[26]  W. Atchley,et al.  Developmental quantitative genetics, conditional epigenetic variability and growth in mice. , 1997, Genetics.

[27]  L. R. Veldboom,et al.  Genetic Mapping of Quantitative Trait Loci in Maize in Stress and Nonstress Environments: I. Grain Yield and Yield Components , 1996 .

[28]  E. Lander,et al.  Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. , 1989, Genetics.

[29]  R. Shoemaker,et al.  Analysis of a Quantitative Trait Locus Allele from Wild Soybean That Increases Seed Protein Concentration in Soybean , 2000 .

[30]  R. Doerge,et al.  Empirical threshold values for quantitative trait mapping. , 1994, Genetics.

[31]  K. Chase,et al.  Genetics of soybean agronomic traits: I. Comparison of three related recombinant inbred populations , 1999 .

[32]  R. Shoemaker,et al.  RFLP analysis of soybean seed protein and oil content , 1992, Theoretical and Applied Genetics.

[33]  J. Zhu,et al.  Quantitative trait loci analysis for the developmental behavior of tiller number in rice (Oryza sativa L.) , 1998, Theoretical and Applied Genetics.

[34]  James E. Specht,et al.  Soybean response to water : A QTL analysis of drought tolerance , 2001 .

[35]  J. Vollmann,et al.  The presence or absence of the soybean Kunitz trypsin inhibitor as a quantitative trait locus for seed protein content , 2002 .