Resource Whole-Brain Imaging with Single-Cell Resolution Using Chemical Cocktails and Computational Analysis

Etsuo A. Susaki,1,2,3,4,14 Kazuki Tainaka,1,3,4,14 Dimitri Perrin,2,14 Fumiaki Kishino,5 Takehiro Tawara,6 Tomonobu M. Watanabe,7 Chihiro Yokoyama,8 Hirotaka Onoe,8 Megumi Eguchi,9 Shun Yamaguchi,9,10 Takaya Abe,11 Hiroshi Kiyonari,11 Yoshihiro Shimizu,12 Atsushi Miyawaki,13 Hideo Yokota,6 and Hiroki R. Ueda1,2,3,4,* 1Laboratory for Synthetic Biology, RIKEN Quantitative Biology Center 2Laboratory for Systems Biology, RIKEN Center for Developmental Biology 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan 3DepartmentofSystemsPharmacology,GraduateSchoolofMedicine, TheUniversityofTokyo, 7-3-1Hongo,Bunkyo-ku,Tokyo113-0033,Japan 4CREST, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan 5Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan 6Image Processing Research Team, RIKEN Center for Advanced Photonics, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan 7Laboratory for Comprehensive Bioimaging, RIKEN Quantitative Biology Center, OLABB, Osaka University, 6-2-3, Furuedai, Suita, Osaka 565-0874, Japan 8Bio-Function Imaging Team, RIKEN Center for Life Science Technologies, 6-7-3 Minatojima-minamimachi, Chuo-ku, Kobe City, Hyogo 650-0047, Japan 9Division of Morphological Neuroscience, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu City, Gifu 501-1194, Japan 10PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012, Japan 11Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan 12Laboratory for Cell-Free Protein Synthesis, RIKEN Quantitative Biology Center, 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo 650-0047, Japan 13Laboratory for Cell Function Dynamics, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan 14Co-first author *Correspondence: uedah-tky@umin.ac.jp http://dx.doi.org/10.1016/j.cell.2014.03.042