Photobiological modulation of cell attachment via cytochrome c oxidase

The number of cells attached to glass substrates increases if HeLa cell suspensions are irradiated with monochromatic visible-to-near infrared radiation (600-860 nm, 52 J m(-2)) prior to plating. The well-structured relationship between this biological response and the radiation wavelength (action spectrum with maxima at 620, 680, 760, and 820 nm) suggests the existence of a photoacceptor responsible for the enhancement of attachment (presumably cytochrome c oxidase, the terminal enzyme of the respiratory chain) and, secondly, the existence of signaling pathways between the mitochondria, the plasma membrane, and the nucleus of the cell. Treating the cell suspension with ouabain (a Na(+), K(+)-ATPase inhibitor), amiloride (an inhibitor of N(+)/H(+) exchangers), or sodium azide (a cytochrome c oxidase inhibitor) prior to irradiation significantly modifies the action spectrum of cell attachment enhancement. The action of the chemicals under study also depends on their concentration and radiation fluence. Our results point to the existence of at least three signaling pathways (reaction channels) relating together the cell attachment, the respiratory chain, and the Na(+), K(+)-ATPase and N(+)/H(+) exchanger activities.

[1]  Zijian Xie,et al.  Na(+)/K(+)-ATPase as a signal transducer. , 2002, European journal of biochemistry.

[2]  T. Karu,et al.  Cell attachment modulation by radiation from a pulsed light diode (λ = 820 nm) and various chemicals , 2001 .

[3]  C. Levinson,et al.  The role of ion transport in the regulation of respiration in the Ehrlich mouse ascites-tumor cell. , 1967, Biochimica et biophysica acta.

[4]  G. Palmer,et al.  Current issues in the chemistry of cytochromec oxidase , 1993, Journal of bioenergetics and biomembranes.

[5]  T. Karu,et al.  Primary and secondary mechanisms of action of visible to near-IR radiation on cells. , 1999, Journal of photochemistry and photobiology. B, Biology.

[6]  S. Grinstein,et al.  Na+/H+ Exchangers of Mammalian Cells* , 1997, The Journal of Biological Chemistry.

[7]  L. Weiss Studies on cellular adhesion in tissue culture. XI. Some effects of ouabain. , 1972, Experimental cell research.

[8]  L. Dalgarno,et al.  Biochemical aspects of the attachment of a pig-kidney monolayer cell line to glass surfaces. , 1971, Experimental Cell Research.

[9]  A. Aperia,et al.  Changes in Na(+)-K(+)-ATPase activity influence cell attachment to fibronectin. , 2002, American journal of physiology. Cell physiology.

[10]  T. Karu,et al.  Photobiology of low-power laser effects. , 1989, Health physics.

[11]  R. Klebe Cell attachment to collagen: the requirement for energy. , 1975, Journal of cellular physiology.

[12]  Jang-Soo Chun,et al.  Requirement for diacylglycerol and protein kinase C in HeLa cell-substratum adhesion and their feedback amplification of arachidonic acid production for optimum cell spreading. , 1993, Molecular biology of the cell.

[13]  M. Humphries,et al.  The structure of cell-adhesion molecules. , 1998, Trends in cell biology.

[14]  C. Lechene,et al.  Adhesion is required for protein kinase C-dependent activation of the Na+/H+ antiporter by platelet-derived growth factor. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[15]  C. Szabó,et al.  NHE blockade inhibits chemokine production and NF-kappaB activation in immunostimulated endothelial cells. , 2002, American journal of physiology. Cell physiology.

[16]  L. Shoshani,et al.  Relationship between Na(+),K(+)-ATPase and cell attachment. , 1999, Journal of cell science.

[17]  R. Klebe Cell Attachment to cllagen: The requirement for energy , 1975 .

[18]  Tiina I. Karu,et al.  Irradiation with a diode at 820 nm induces changes in circular dichroism spectra (250-780 nm) of living cells , 2001, European Conference on Biomedical Optics.

[19]  L. Margolis,et al.  Intracellular pH and cell adhesion to solid substrate , 1988, FEBS letters.

[20]  Semion Rochkind,et al.  Effects of laser irradiation on the spinal cord for the regeneration of crushed peripheral nerve in rats , 2001, Lasers in surgery and medicine.

[21]  A. Askari,et al.  Involvement of Src and epidermal growth factor receptor in the signal-transducing function of Na+/K+-ATPase. , 2000, The Journal of biological chemistry.

[22]  K. Byrnes,et al.  Quantitation of calcitonin gene‐related peptide mrna and neuronal cell death in facial motor nuclei following axotomy and 633 nm low power laser treatment , 2002, Lasers in surgery and medicine.

[23]  L. A. Welser,et al.  Changes in absorbance of monolayer of living cells induced by laser radiation at 633, 670, and 820 nm , 2001, European Conference on Biomedical Optics.

[24]  L. Weiss,et al.  Studies on cellular adhesion in tissue culture. I. The effect of serum. , 1959, Experimental cell research.

[25]  T. Karu,et al.  Melatonin modulates the action of near infrared radiation on cell adhesion , 2003, Journal of pineal research.

[26]  O Einarsdóttir,et al.  Mechanism of cytochrome c oxidase-catalyzed reduction of dioxygen to water: evidence for peroxy and ferryl intermediates at room temperature. , 1997, Biochemistry.

[27]  P. Greengard,et al.  Mutation of the Protein Kinase C Phosphorylation Site on Rat α1 Na+,K+-ATPase Alters Regulation of Intracellular Na+ and pH and Influences Cell Shape and Adhesiveness* , 1997, The Journal of Biological Chemistry.

[28]  B. Jacobson,et al.  Extracellular calcium regulates HeLa cell morphology during adhesion to gelatin: role of translocation and phosphorylation of cytosolic phospholipase A2. , 1998, Molecular biology of the cell.

[29]  A. Schousboe,et al.  Increases in [Ca2+]i and changes in intracellular pH during chemical anoxia in mouse neocortical neurons in primary culture , 1999, Journal of neuroscience research.

[30]  Yuichiro Yamada,et al.  Ouabain suppresses glucose-induced mitochondrial ATP production and insulin release by generating reactive oxygen species in pancreatic islets. , 2002, Diabetes.

[31]  V. S. Letokhov,et al.  Biostimulation of HeLa cells by low-intensity visible light , 1982 .

[32]  Noel T. Whelan,et al.  Therapeutic photobiomodulation for methanol-induced retinal toxicity , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[33]  Uri Oron,et al.  Low-energy laser irradiation promotes the survival and cell cycle entry of skeletal muscle satellite cells. , 2002, Journal of cell science.

[34]  R. Chiquet‐Ehrismann,et al.  Adhesion modulation by antiadhesive molecules of the extracellular matrix. , 2000, Experimental cell research.

[35]  M. Wong-Riley,et al.  Light-emitting diode treatment reverses the effect of TTX on cytochrome oxidase in neurons , 2001, Neuroreport.

[36]  U. Oron,et al.  Impact of low level laser irradiation on infarct size in the rat following myocardial infarction. , 2001, International journal of cardiology.

[37]  R. Esenaliev,et al.  Effects of monochromatic low‐intensity light and laser irradiation on adhesion of HeLa cells in vitro , 1996, Lasers in surgery and medicine.

[38]  L. Margolis,et al.  Regulation of intracellular pH by phospholipase A2 and protein kinase C upon neutrophil adhesion to solid substrata , 1996, FEBS letters.