Distinct behavior of beta-endorphin and corticotropin toward leucine aminopeptidase action.

Reactions of human beta-endorphin, corticotropin and their synthetic analogs with leucine aminopeptidase have been investigated. The results confirmed previous findings that beta-endorphin is resistant to the aminopeptidase action whereas corticotropin is not. Beta-endorphin-(1-5) is completely digested by the enzyme while beta-endorphin-(1-17) is resistant. In contrast, the NH2-terminal 7 residues in corticotropin are removed readily by leucine aminopeptidase. This is confirmed by the observation that human corticotropin-(7-38) is not hydrolyzed by the enzyme. This contrasting behavior of the two hormones toward leucine aminopeptidase may be related to differences in their conformational structures.

[1]  L. Nádasdi,et al.  Enkephalin analogs: synthesis and properties of analogs with lipophilic or extended carboxyl-terminus. Quantitative structure-activity relationship of analogs modified in residue position 5. , 2009, International journal of peptide and protein research.

[2]  C. Li,et al.  Isolation, characterization and amino acid sequence of beta-lipotropin from human pituitary glands. , 2009, International journal of peptide and protein research.

[3]  Choh Hao Li,et al.  β-Endorphin: Analgesic and hormonal effects in humans , 1979 .

[4]  C. Li,et al.  Isolation, characterization, and synthesis of a corticotropin-inhibiting peptide from human pituitary glands. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[5]  H. Loh,et al.  Synthesis and analgesic activity of human beta-endorphin. , 1977, Journal of medicinal chemistry.

[6]  H. Loh,et al.  beta-Endorphin: structure-activity relationships in the guinea pig ileum and opiate receptor binding assays. , 1977, Biochemical and biophysical research communications.

[7]  J. Ramachandran,et al.  ADRENOCORTICOTROPINS. 33. SYNTHESIS OF A BIOLOGICALLY ACTIVE HEXACOSAPEPTIDE CORRESPONDING TO THE FIRST 26 RESIDUES OF BOVINE ACTH. , 1965, Journal of the American Chemical Society.

[8]  Choh Hao Li,et al.  Synthesis of L-Methionyl-L-Glutamyl-L-Histidyl-L-Phenylalanyl-L-Arginyl-L-Tryptophyl-Glycine and its Melanocyte-stimulating and Corticotropin-releasing Activity , 1961, Nature.

[9]  D. Chung,et al.  Corticotropins (ACTH). XV. The action of chymotrypsin on α-corticotropin , 1959 .

[10]  W. White,et al.  STUDIES ON ADRENOCORTICOTROPIN. XII. ACTION OF AMINOPEPTIDASE ON CORTICOTROPIN-A; EFFECT ON BIOLOGICAL ACTIVITY , 1955 .