Radioimmunological determination and characterization of cathodal trypsin-like immunoreactivity in normal human plasma.

A radioimmunological method for determination of human cathodal trypsin-like immunoreactivity is described. DFP-treated human cathodal trypsin is used as standard and tracer. Freshly drawn normal human plasma contains about 25 microgram/l of cathodal trypsin-like immunoreactivity measured as DFP-treated cathodal trypsin. The normally circulating cathodal trypsin-like immunoreactivity is shown to consist mainly of cathodal trypsinogen.

[1]  K. Ohlsson,et al.  Demonstration and semiquantitative determination of complexes between various proteases and human alpha2-macroglobulin. , 1976, Clinica chimica acta; international journal of clinical chemistry.

[2]  J. Howard,et al.  Radioimmunoassay of Plasma Elastase , 1974, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[3]  J. Howard,et al.  The twenty-four excretion of trypsin and trypsinogen in the urine. , 1972, Annals of surgery.

[4]  J. Thorell,et al.  Enzymatic iodination of polypeptides with 125I to high specific activity. , 1971, Biochimica et biophysica acta.

[5]  M. Steinbuch,et al.  The isolation of IgG from mammalian sera with the aid of caprylic acid. , 1969, Archives of biochemistry and biophysics.

[6]  William W. Cohen,et al.  The preparation and properties of two new chromogenic substrates of trypsin. , 1961, Archives of biochemistry and biophysics.

[7]  M. Richardson.  Fracture of the Pelvis, Laceration of the Vaginal Wall and Protrusion of the Intestines , 1888 .

[8]  H. Bennich,et al.  Human Antibodies to Bovine γ-Globulin , 1975 .

[9]  B G Johansson,et al.  Agarose gel electrophoresis. , 1972, Scandinavian journal of clinical and laboratory investigation. Supplementum.

[10]  Andrzej Szczelik.  Trypsin-like activity of serum: its origin and electrophoretic separation. , 1969 .

[11]  J. Scheidegger.  Une micro-méthode de l’immuno-électrophorèse , 1955 .